Logo of jvirolPermissionsJournals.ASM.orgJournalJV ArticleJournal InfoAuthorsReviewers
J Virol. Dec 1980; 36(3): 878–882.
PMCID: PMC353715

Cloned human polyomavirus JC DNA can transform human amnion cells.


The genome of the human polyomavirus JC (Mad-1 strain) was molecularly cloned in Escherichia coli by using the plasmid vector pBR322. Recombinant DNA molecules were constructed with the entire JC genome inserted either at its unique EcoRI site at 0.0 map units or at its unique BamHI site at 0.51 map units. Viral DNA from each of these recombinant plasmids was capable of transforming human amnion cells, and cell lines established from transformed foci were positive for JC tumor antigen as assayed by indirect immunofluorescence.

Full text

Full text is available as a scanned copy of the original print version. Get a printable copy (PDF file) of the complete article (947K), or click on a page image below to browse page by page. Links to PubMed are also available for Selected References.

Images in this article

Click on the image to see a larger version.

Selected References

These references are in PubMed. This may not be the complete list of references from this article.
  • Bolivar F, Rodriguez RL, Greene PJ, Betlach MC, Heyneker HL, Boyer HW, Crosa JH, Falkow S. Construction and characterization of new cloning vehicles. II. A multipurpose cloning system. Gene. 1977;2(2):95–113. [PubMed]
  • Brown P, Tsai T, Gajdusek DC. Seroepidemiology of human papovaviruses. Discovery of virgin populations and some unusual patterns of antibody prevalence among remote peoples of the world. Am J Epidemiol. 1975 Oct;102(4):331–340. [PubMed]
  • Clewell DB. Nature of Col E 1 plasmid replication in Escherichia coli in the presence of the chloramphenicol. J Bacteriol. 1972 May;110(2):667–676. [PMC free article] [PubMed]
  • Coleman DV, Daniel RA, Gardner SD, Field AM, Gibson PE. Polyoma virus in urine during pregnancy. Lancet. 1977 Oct 1;2(8040):709–710. [PubMed]
  • Frisque RJ, Martin JD, Padgett BL, Walker DL. Infectivity of the DNA from four isolates of JC virus. J Virol. 1979 Nov;32(2):476–482. [PMC free article] [PubMed]
  • Frisque RJ, Rifkin DB, Walker DL. Transformation of primary hamster brain cells with JC virus and its DNA. J Virol. 1980 Jul;35(1):265–269. [PMC free article] [PubMed]
  • Gardner SD, Field AM, Coleman DV, Hulme B. New human papovavirus (B.K.) isolated from urine after renal transplantation. Lancet. 1971 Jun 19;1(7712):1253–1257. [PubMed]
  • Graham FL, van der Eb AJ. A new technique for the assay of infectivity of human adenovirus 5 DNA. Virology. 1973 Apr;52(2):456–467. [PubMed]
  • Grunstein M, Hogness DS. Colony hybridization: a method for the isolation of cloned DNAs that contain a specific gene. Proc Natl Acad Sci U S A. 1975 Oct;72(10):3961–3965. [PMC free article] [PubMed]
  • Hogan TF, Borden EC, McBain JA, Padgett BL, Walker DL. Human polyomavirus infections with JC virus and BK virus in renal transplant patients. Ann Intern Med. 1980 Mar;92(3):373–378. [PubMed]
  • Howley PM, Israel MA, Law MF, Martin MA. A rapid method for detecting and mapping homology between heterologous DNAs. Evaluation of polyomavirus genomes. J Biol Chem. 1979 Jun 10;254(11):4876–4883. [PubMed]
  • Israel MA, Chan HW, Rowe WP, Martin MA. Molecular cloning of polyoma virus DNA in Escherichia coli: plasmid vector system. Science. 1979 Mar 2;203(4383):883–887. [PubMed]
  • Law MF, Martin JD, Takemoto KK, Howley PM. The colinear alignment of the genomes of papovaviruses JC, BK, and SV40. Virology. 1979 Jul 30;96(2):576–587. [PubMed]
  • London WT, Houff SA, Madden DL, Fuccillo DA, Gravell M, Wallen WC, Palmer AE, Sever JL, Padgett BL, Walker DL, et al. Brain tumors in owl monkeys inoculated with a human polyomavirus (JC virus). Science. 1978 Sep 29;201(4362):1246–1249. [PubMed]
  • Martin JD, Frisque RJ, Padgett BL, Walker DL. Restriction endonuclease cleavage map of the DNA of JC virus. J Virol. 1979 Mar;29(3):846–855. [PMC free article] [PubMed]
  • McCutchan JH, Pagano JS. Enchancement of the infectivity of simian virus 40 deoxyribonucleic acid with diethylaminoethyl-dextran. J Natl Cancer Inst. 1968 Aug;41(2):351–357. [PubMed]
  • Miyamura T, Yoshiike K, Takemoto KK. Characterization of JC papovavirus adapted to growth in human embryonic kidney cells. J Virol. 1980 Aug;35(2):498–504. [PMC free article] [PubMed]
  • Padgett BL, Walker DL. Prevalence of antibodies in human sera against JC virus, an isolate from a case of progressive multifocal leukoencephalopathy. J Infect Dis. 1973 Apr;127(4):467–470. [PubMed]
  • Padgett BL, Walker DL. New human papovaviruses. Prog Med Virol. 1976;22:1–35. [PubMed]
  • Padgett BL, Walker DL, ZuRhein GM, Eckroade RJ, Dessel BH. Cultivation of papova-like virus from human brain with progressive multifocal leucoencephalopathy. Lancet. 1971 Jun 19;1(7712):1257–1260. [PubMed]
  • Padgett BL, Walker DL, ZuRhein GM, Hodach AE, Chou SM. JC Papovavirus in progressive multifocal leukoencephalopathy. J Infect Dis. 1976 Jun;133(6):686–690. [PubMed]
  • Padgett BL, Walker DL, ZuRhein GM, Varakis JN. Differential neurooncogenicity of strains of JC virus, a human polyoma virus, in newborn Syrian hamsters. Cancer Res. 1977 Mar;37(3):718–720. [PubMed]
  • Purchio AF, Fareed GC. Transformation of human embryonic kidney cells by human papovarirus BK. J Virol. 1979 Feb;29(2):763–769. [PMC free article] [PubMed]
  • Rigby PW, Dieckmann M, Rhodes C, Berg P. Labeling deoxyribonucleic acid to high specific activity in vitro by nick translation with DNA polymerase I. J Mol Biol. 1977 Jun 15;113(1):237–251. [PubMed]
  • Southern EM. Detection of specific sequences among DNA fragments separated by gel electrophoresis. J Mol Biol. 1975 Nov 5;98(3):503–517. [PubMed]
  • Takemoto KK, Howley PM, Miyamura T. JC human papovavirus replication in human amnion cells. J Virol. 1979 Apr;30(1):384–389. [PMC free article] [PubMed]
  • Takemoto KK, Linke H, Miyamura T, Fareed GC. Persistent BK papovavirus infection of transformed human fetal brain cells. I. Episomal viral DNA in cloned lines deficient in T-antigen expression. J Virol. 1979 Mar;29(3):1177–1185. [PMC free article] [PubMed]
  • Varakis JN, ZuRhein GM. Experimental pineocytoma of the Syrian hamster induced by a human papovavirus (JC). A light and electron microscopic study. Acta Neuropathol. 1976;35(3):243–264. [PubMed]
  • Walker DL, Padgett BL, ZuRhein GM, Albert AE, Marsh RF. Human papovavirus (JC): induction of brain tumors in hamsters. Science. 1973 Aug 17;181(4100):674–676. [PubMed]
  • Wensink PC, Finnegan DJ, Donelson JE, Hogness DS. A system for mapping DNA sequences in the chromosomes of Drosophila melanogaster. Cell. 1974 Dec;3(4):315–325. [PubMed]

Articles from Journal of Virology are provided here courtesy of American Society for Microbiology (ASM)


Related citations in PubMed

See reviews...See all...

Cited by other articles in PMC

See all...


Recent Activity

Your browsing activity is empty.

Activity recording is turned off.

Turn recording back on

See more...