• We are sorry, but NCBI web applications do not support your browser and may not function properly. More information
Logo of jvirolPermissionsJournals.ASM.orgJournalJV ArticleJournal InfoAuthorsReviewers
J Virol. Jun 1979; 30(3): 863–874.
PMCID: PMC353397

Reovirus serotypes 1 and 3 differ in their in vitro association with microtubules.

Abstract

Utilizing negative-stain electron microscopy in which similar concentrations of reovirus types 1 and 3 are incubated with a carbon support film containing chick brain, rabbit brain, or HeLa cell microtubules, 81% of the type 1 and 56% of type 3 exhibited an association with the apparent "edge" of the microtubule. This implies that there is a high level of specific affinity for type 1 but not for type 3 to microtubules, since it has previously been determined that only 50% of randomly associated particles would be associated with the edge. The high edge binding of reovirus type 1 is virtually independent of the origin of microtubule, or of whether microtubules or virus has been initially adhered to the support film. On the other hand, reovirus type 1-specific antiserum reduced the edge binding or reovirus type 1 to 45%, whereas type 3 specific antiserum caused no less (within the variability of the assay) of the edge binding of reovirus type 1 to microtubules (76% edge bound). High edge binding of reovirus type 1 to microtubules is correlated with the presence of type 1 or sigma 1 polypeptide. This minor outer capsid polypeptide is encoded in the S1 double-stranded RNA segment and is the viral hemagglutinin and neutralization antigen. Recombinant reovirus clones containing the S1 double-stranded RNA segment of type 1 (80 and 802) show about 85% edge binding, as compared to a value of 42% for clones and the S1 gene of type 3 (204. Electron microscopy of purified reovirus types 1 and 3 by negative staining reveals that type 1 and 802 capsomers are distinctly visualized, whereas those of type 3 and 204 appear diffuse. Thus, the greater in vitro binding of type 1 to microtubules may reflect an increased accessibility of certain of its outer capsomers, and thereby, sigma 1 polypeptides to microtubules. Examination of its outer sections of reovirus type 1- and 3-infected cells at 24 to 48 h postinfection at 31 degrees C showed that about eight times as many viral factoris in type 1-infected cells exhibited an extensive association of virus particles with microtubules, as compared to viral factories of type 3-infected cells. Thus, both in vivo and in vitro there appears to be a greater specificity for the association of reovirus type 1 particles with microtubules, as compared to reovirus type 3 particles.

Full text

Full text is available as a scanned copy of the original print version. Get a printable copy (PDF file) of the complete article (3.1M), or click on a page image below to browse page by page. Links to PubMed are also available for Selected References.

Images in this article

Selected References

These references are in PubMed. This may not be the complete list of references from this article.
  • Amin M, Mirza A, Weber J. Genetic analysis of adenovirus type 2. VII. Cleavage-modified affinity for DNA of internal virion proteins. Virology. 1977 Jul 1;80(1):83–97. [PubMed]
  • Banerjee AK, Shatkin AJ. Transcription in vitro by reovirus-associated ribonucleic acid-dependent polymerase. J Virol. 1970 Jul;6(1):1–11. [PMC free article] [PubMed]
  • Both GW, Lavi S, Shatkin AJ. Synthesis of all the gene products of the reovirus genome in vivo and in vitro. Cell. 1975 Feb;4(2):173–180. [PubMed]
  • DALES S. ASSOCIATION BETWEEN THE SPINDLE APPARATUS AND REOVIRUS. Proc Natl Acad Sci U S A. 1963 Aug;50:268–275. [PMC free article] [PubMed]
  • Dales S, Chardonnet Y. Early events in the interaction of adenoviruses with HeLa cells. IV. Association with microtubules and the nuclear pore complex during vectorial movement of the inoculum. Virology. 1973 Dec;56(2):465–483. [PubMed]
  • DALES S, GOMATOS PJ, HSU KC. THE UPTAKE AND DEVELOPMENT OF REOVIRUS IN STRAIN L CELLS FOLLOWED WITH LABELED VIRAL RIBONUCLEIC ACID AND FERRITIN-ANTIBODY CONJUGATES. Virology. 1965 Feb;25:193–211. [PubMed]
  • Dentler WL, Granett S, Rosenbaum JL. Ultrastructural localization of the high molecular weight proteins associated with in vitro-assembled brain microtubules. J Cell Biol. 1975 Apr;65(1):237–241. [PMC free article] [PubMed]
  • Joklik WK. The molecular biology of Reovirus. J Cell Physiol. 1970 Dec;76(3):289–301. [PubMed]
  • Luftig RB, Weihing RR. Adenovirus binds to rat brain microtubules in vitro. J Virol. 1975 Sep;16(3):696–706. [PMC free article] [PubMed]
  • Luftig RB, McMillan PN, Weatherbee JA, Weihing RR. Increased visualization of microtubules by an improved fixation procedure. J Histochem Cytochem. 1977 Mar;25(3):175–187. [PubMed]
  • Luftig RB, Kilham SS, Hay AJ, Zweerink HJ, Joklik WK. An ultrastructural study of virions and cores of reovirus type 3. Virology. 1972 Apr;48(1):170–181. [PubMed]
  • McCrae MA, Joklik WK. The nature of the polypeptide encoded by each of the 10 double-stranded RNA segments of reovirus type 3. Virology. 1978 Sep;89(2):578–593. [PubMed]
  • Murphy DB, Borisy GG. Association of high-molecular-weight proteins with microtubules and their role in microtubule assembly in vitro. Proc Natl Acad Sci U S A. 1975 Jul;72(7):2696–2700. [PMC free article] [PubMed]
  • Mustoe TA, Ramig RF, Sharpe AH, Fields BN. Genetics of reovirus: identification of the ds RNA segments encoding the polypeptides of the mu and sigma size classes. Virology. 1978 Sep;89(2):594–604. [PubMed]
  • Ramig RF, Cross RK, Fields BN. Genome RNAs and polypeptides of reovirus serotypes 1, 2, and 3. J Virol. 1977 Jun;22(3):726–733. [PMC free article] [PubMed]
  • Sharpe AH, Ramig RF, Mustoe TA, Fields BN. A genetic map of reovirus. 1. Correlation of genome RNAs between serotypes 1, 2, and 3. Virology. 1978 Jan;84(1):63–74. [PubMed]
  • Shatkin AJ, Sipe JD. RNA polymerase activity in purified reoviruses. Proc Natl Acad Sci U S A. 1968 Dec;61(4):1462–1469. [PMC free article] [PubMed]
  • Shatkin AJ, Sipe JD, Loh P. Separation of ten reovirus genome segments by polyacrylamide gel electrophoresis. J Virol. 1968 Oct;2(10):986–991. [PMC free article] [PubMed]
  • Silverstein SC, Dales S. The penetration of reovirus RNA and initiation of its genetic function in L-strain fibroblasts. J Cell Biol. 1968 Jan;36(1):197–230. [PMC free article] [PubMed]
  • Smith RE, Zweerink HJ, Joklik WK. Polypeptide components of virions, top component and cores of reovirus type 3. Virology. 1969 Dec;39(4):791–810. [PubMed]
  • SPENDLOVE RS, LENNETTE EH, CHIN JN, KNIGHT CO. EFFECT OF ANTIMITOTIC AGENTS ON INTRACELLULAR REOVIRUS ANTIGEN. Cancer Res. 1964 Nov;24:1826–1833. [PubMed]
  • SPENDLOVE RS, LENNETTE EH, JOHN AC. THE ROLE OF THE MITOTIC APPARATUS IN THE INTRACELLULAR LOCATION OF REOVIRUS ANTIGEN. J Immunol. 1963 Apr;90:554–560. [PubMed]
  • Starger JM, Goldman RD. Isolation and preliminary characterization of 10-nm filaments from baby hamster kidney (BHK-21) cells. Proc Natl Acad Sci U S A. 1977 Jun;74(6):2422–2426. [PMC free article] [PubMed]
  • Weatherbee JA, Luftig RB, Weihing RR. Binding of adenovirus to microtubules. II. Depletion of high-molecular-weight microtubule-associated protein content reduces specificity of in vitro binding. J Virol. 1977 Feb;21(2):732–742. [PMC free article] [PubMed]
  • Weatherbee JA, Luftig RB, Weihing RR. In vitro polymerization of microtubules from HeLa cells. J Cell Biol. 1978 Jul;78(1):47–57. [PMC free article] [PubMed]
  • Weiner HL, Drayna D, Averill DR, Jr, Fields BN. Molecular basis of reovirus virulence: role of the S1 gene. Proc Natl Acad Sci U S A. 1977 Dec;74(12):5744–5748. [PMC free article] [PubMed]
  • Weiner HL, Fields BN. Neutralization of reovirus: the gene responsible for the neutralization antigen. J Exp Med. 1977 Nov 1;146(5):1305–1310. [PMC free article] [PubMed]
  • Weiner HL, Ramig RF, Mustoe TA, Fields BN. Identification of the gene coding for the hemagglutinin of reovirus. Virology. 1978 May 15;86(2):581–584. [PubMed]
  • Yanagida M, Ahmad-Zadeh C. Determination of gene product positions in bacteriophage T4 by specific antibody association. J Mol Biol. 1970 Jul 28;51(2):411–421. [PubMed]

Articles from Journal of Virology are provided here courtesy of American Society for Microbiology (ASM)

Formats:

Related citations in PubMed

Cited by other articles in PMC

See all...

Links

Recent Activity

Your browsing activity is empty.

Activity recording is turned off.

Turn recording back on

See more...