Logo of aacPermissionsJournals.ASM.orgJournalAAC ArticleJournal InfoAuthorsReviewers
Antimicrob Agents Chemother. 1977 Jul; 12(1): 11–15.
PMCID: PMC352146

Human Interferon: Mass Production in a Newly Established Cell Line, MG-63


MG-63 cells, a line derived from an osteosarcoma, produced high yields of interferon after superinduction with polyinosinic acid·polycytidylic acid, cycloheximide, and actinomycin D. Advantages of MG-63 cells over diploid fibroblasts as a substrate are: no requirement for aging between confluency and induction, no requirement for priming, and 3.7-fold higher yields per square centimeter of culture surface. Physicochemically and biologically, MG-63 cell interferon resembles fibroblast rather than leukocyte interferon.

Full text

Full text is available as a scanned copy of the original print version. Get a printable copy (PDF file) of the complete article (870K), or click on a page image below to browse page by page. Links to PubMed are also available for Selected References.

Selected References

These references are in PubMed. This may not be the complete list of references from this article.
  • Billiau A, Cassiman JJ, Willems D, Verhelst M, Heremans H. In vitro cultivation of human tumor tissues. Oncology. 1975;31(5-6):257–272. [PubMed]
  • Billiau A, Joniau M, De Somer P. Mass production of human interferon in diploid cells stimulated by poly-I:C. J Gen Virol. 1973 Apr;19(1):1–8. [PubMed]
  • Cantell K, Hirvonen S, Mogensen KE, Pyhälä L. Human leukocyte interferon: production, purification, stability, and animal experiments. In Vitro Monogr. 1974;(3):35–38. [PubMed]
  • Desmyter J, De Groote J, Desmet VJ, Billiau A, Ray MB, Bradburne AF, Edy VG, De Somer P. Administration of human fibroblast interferon in chronic hepatitis-B infection. Lancet. 1976 Sep 25;2(7987):645–647. [PubMed]
  • Desmyter J, Stewart WE., 2nd Molecular modification of interferon: attainment of human interferon in a conformation active on cat cells but inactive on human cells. Virology. 1976 Apr;70(2):451–458. [PubMed]
  • De Somer P, Joniau M, Edy VG, Billiau A. Mass production of human interferon in diploid cells. In Vitro Monogr. 1974;(3):39–46. [PubMed]
  • Edy VG, Braude IA, De Clercq E, Billiau A, De Somer P. Purification of interferon by adsorption chromatography on controlled pore glass. J Gen Virol. 1976 Dec;33(3):517–521. [PubMed]
  • Gresser I, Bandu MT, Brouty-boye D, Tovey M. Pronounced antiviral activity of human interferon on bovine and porcine cells. Nature. 1974 Oct 11;251(5475):543–545. [PubMed]
  • Havell EA, Berman B, Ogburn CA, Berg K, Paucker K, Vilcek J. Two antigenically distinct species of human interferon. Proc Natl Acad Sci U S A. 1975 Jun;72(6):2185–2187. [PMC free article] [PubMed]
  • Havell EA, Vilcek J. Production of high-titered interferon in cultures of human diploid cells. Antimicrob Agents Chemother. 1972 Dec;2(6):476–484. [PMC free article] [PubMed]
  • Mogensen KE, Pyhälä L, Cantell K. Raising antibodies to human leukocyte interferon. Acta Pathol Microbiol Scand B. 1975 Oct;83(5):443–450. [PubMed]
  • Strander H, Mogensen KE, Cantell K. Production of human lymphoblastoid interferon. J Clin Microbiol. 1975 Jan;1(1):116–117. [PMC free article] [PubMed]
  • Tan YH, Armstrong JA, Ke YH, Ho M. Regulation of cellular interferon production: enhancement by antimetabolites. Proc Natl Acad Sci U S A. 1970 Sep;67(1):464–471. [PMC free article] [PubMed]
  • Tan YH, Creagan RP, Ruddle FH. The somatic cell genetics of human interferon: assignment of human interferon loci to chromosomes 2 and 5. Proc Natl Acad Sci U S A. 1974 Jun;71(6):2251–2255. [PMC free article] [PubMed]

Articles from Antimicrobial Agents and Chemotherapy are provided here courtesy of American Society for Microbiology (ASM)


Save items

Related citations in PubMed

See reviews...See all...

Cited by other articles in PMC

See all...


  • MedGen
    Related information in MedGen
  • PubMed
    PubMed citations for these articles
  • Substance
    PubChem chemical substance records that cite the current articles. These references are taken from those provided on submitted PubChem chemical substance records.

Recent Activity

Your browsing activity is empty.

Activity recording is turned off.

Turn recording back on

See more...