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Antimicrob Agents Chemother. Jun 1977; 11(6): 946–951.
PMCID: PMC352108

Inhibition of Influenza Virus Ribonucleic Acid Polymerase by Ribavirin Triphosphate


Ribavirin 5′-triphosphate (RTP), derived from the broad-spectrum antiviral compound ribavirin (Virazole), can selectively inhibit influenza virus ribonucleic acid polymerase in a cell-free assay. Ribavirin and its 5′-monophosphate have no effect on the polymerase. The inhibition is competitive with respect to adenosine 5′-triphosphate and guanosine 5′-triphosphate. RTP also inhibits ApG- and GpC-stimulated influenza virus ribonucleic acid polymerase. Since ribavirin is phosphorylated in the cell, the inhibition of influenza multiplication in the cell may also be caused by RTP.

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Selected References

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  • Baltimore D, Huang AS, Stampfer M. Ribonucleic acid synthesis of vesicular stomatitis virus, II. An RNA polymerase in the virion. Proc Natl Acad Sci U S A. 1970 Jun;66(2):572–576. [PMC free article] [PubMed]
  • Bishop DH, Obijeski JF, Simpson RW. Transcription of the influenza ribonucleic acid genome by a virion polymerase. I. Optimal conditions for in vitro activity of the ribonucleic acid-dependent ribonucleic acid polymerase. J Virol. 1971 Jul;8(1):66–73. [PMC free article] [PubMed]
  • Burgess RR. A new method for the large scale purification of Escherichia coli deoxyribonucleic acid-dependent ribonucleic acid polymerase. J Biol Chem. 1969 Nov 25;244(22):6160–6167. [PubMed]
  • Fansler BS, Loeb LA. Sea urchin nuclear DNA polymerase. Methods Enzymol. 1974;29:53–70. [PubMed]
  • Harwood SJ, Schendel PF, Wells RD. Micrococcus luteus deoxyribonucleic acid polymerase. Studies of the enzymic reaction and properties of the deoxyribonucleic acid product. J Biol Chem. 1970 Nov 10;245(21):5614–5624. [PubMed]
  • Holmes AM, Hesslewood IP, Johnston IR. Evidence that DNA polymerase-alpha of calf thymus contains a subunit of molecular weight 155000. Eur J Biochem. 1976 Feb 16;62(2):229–235. [PubMed]
  • Kedinger C, Gissinger F, Gniazdowski M, Mandel JL, Chambon P. Animal DNA-dependent RNA polymerases. 1. Large-scale solubilization and separation of A and B calf-thymus RNA-polymerase activities. Eur J Biochem. 1972 Jul 13;28(2):269–276. [PubMed]
  • Krug RM, Morgan MA, Shatkin AJ. Influenza viral mRNA contains internal N6-methyladenosine and 5'-terminal 7-methylguanosine in cap structures. J Virol. 1976 Oct;20(1):45–53. [PMC free article] [PubMed]
  • McGeoch D, Kitron N. Influenza virion RNA-dependent RNA polymerase: stimulation by guanosine and related compounds. J Virol. 1975 Apr;15(4):686–695. [PMC free article] [PubMed]
  • Oxford JS. Inhibition of the replication of influenza A and B viruses by a nucleoside analogue (ribavirin). J Gen Virol. 1975 Sep;28(3):409–414. [PubMed]
  • Plotch SJ, Krug RM. Influenza virion transcriptase: synthesis in vitro of large, polyadenylic acid-containing complementary RNA. J Virol. 1977 Jan;21(1):24–34. [PMC free article] [PubMed]
  • Pons MW, Hirst GK. Polyacrylamide gel electrophoresis of influenza virus RNA. Virology. 1968 Feb;34(2):385–388. [PubMed]
  • Roy P, Bishop DH. Genome homology of vesicular stomatitis virus and defective T particles and evidence for the sequential transcription of the virion ribonucleic acid. J Virol. 1972 Jun;9(6):946–955. [PMC free article] [PubMed]
  • Sidwell RW, Huffman JH, Khare GP, Allen LB, Witkowski JT, Robins RK. Broad-spectrum antiviral activity of Virazole: 1-beta-D-ribofuranosyl-1,2,4-triazole-3-carboxamide. Science. 1972 Aug 25;177(4050):705–706. [PubMed]
  • Scholtissek C. Inhibition of influenza RNA synthesis by virazole (ribavirin). Arch Virol. 1976;50(4):349–352. [PubMed]
  • Streeter DG, Witkowski JT, Khare GP, Sidwell RW, Bauer RJ, Robins RK, Simon LN. Mechanism of action of 1- -D-ribofuranosyl-1,2,4-triazole-3-carboxamide (Virazole), a new broad-spectrum antiviral agent. Proc Natl Acad Sci U S A. 1973 Apr;70(4):1174–1178. [PMC free article] [PubMed]
  • Witkowski JT, Robins RK, Sidwell RW, Simon LN. Design, synthesis, and broad spectrum antiviral activity of 1- -D-ribofuranosyl-1,2,4-triazole-3-carboxamide and related nucleosides. J Med Chem. 1972 Nov;15(11):1150–1154. [PubMed]

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