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Fig. 2.

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G9a is recruited to GR target genes that require G9a for dex-regulated expression. ChIP was performed on A549 cells untreated or treated with 100 nM dex for 0 or 5 h. Immunoprecipitated DNA was analyzed by qPCR by using the indicated primers (sequences given in Dataset S3), and results are normalized to input chromatin and shown as the ratio of 5 h vs. 0 h of dex treatment. Results shown are mean ± SD (n = 3) of PCR reactions from a single experiment, and are representative of at least three independent experiments.

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