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Proc Natl Acad Sci U S A. Oct 1980; 77(10): 6067–6071.
PMCID: PMC350214

Inheritance of chloroplast DNA in Chlamydomonas reinhardtii

Abstract

Two symmetrically located deletions of approximately 100 base pairs each have been identified in chloroplast DNA of Chlamydomonas reinhardtii. Although present in a mutant strain that requires acetate for growth, both deletions have been shown to be distinct from the nonphotosynthetic phenotype of this strain. These physical markers in the chloroplast genome and maternally inherited genetic markers showed strict cotransmission in reciprocal crosses. Thus, our results are consistent with the location of the well-characterized maternally inherited genetic markers in chloroplast DNA of C. reinhardtii.

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Selected References

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  • Sager R. MENDELIAN AND NON-MENDELIAN INHERITANCE OF STREPTOMYCIN RESISTANCE IN CHLAMYDOMONAS REINHARDI. Proc Natl Acad Sci U S A. 1954 May;40(5):356–363. [PMC free article] [PubMed]
  • Shepherd HS, Boynton JE, Gillham NW. Mutations in nine chloroplast loci of Chlamydomonas affecting different photosynthetic functions. Proc Natl Acad Sci U S A. 1979 Mar;76(3):1353–1357. [PMC free article] [PubMed]
  • Chiang KS. Physical conservation of parental cytoplasmic DNA through meiosis in Chlamydomonas reinhardi. Proc Natl Acad Sci U S A. 1968 May;60(1):194–200. [PMC free article] [PubMed]
  • Sueoka N. MITOTIC REPLICATION OF DEOXYRIBONUCLEIC ACID IN CHLAMYDOMONAS REINHARDI. Proc Natl Acad Sci U S A. 1960 Jan;46(1):83–91. [PMC free article] [PubMed]
  • Stafford DW, Bieber D. Concentration of DNA solutions by extraction with 2-butanol. Biochim Biophys Acta. 1975 Jan 6;378(1):18–21. [PubMed]
  • Anet R, Strayer DR. Sodium iodide density gradients for the preparative buoyant density separations of DNA mixtures. Biochem Biophys Res Commun. 1969 Sep 24;37(1):52–58. [PubMed]
  • Vogelstein B, Gillespie D. Preparative and analytical purification of DNA from agarose. Proc Natl Acad Sci U S A. 1979 Feb;76(2):615–619. [PMC free article] [PubMed]
  • McCarty KS, Jr, Vollmer RT, McCarty KS. Improved computer program data for the resolution and fractionation of macromolecules by isokinetic sucrose density gradient sedimentation. Anal Biochem. 1974 Sep;61(1):165–183. [PubMed]
  • DeLeys RJ, Jackson DA. Electrophoretic analysis of covalently closed SV40 DNA: Boltzmann distributions of DNA species. Nucleic Acids Res. 1976 Mar;3(3):641–652. [PMC free article] [PubMed]
  • Grant D, Chiang KS. Physical mapping and characterization of Chlamydomonas mitochondrial DNA molecules: their unique ends, sequence homogeneity, and conservation. Plasmid. 1980 Jul;4(1):82–96. [PubMed]
  • McDonell MW, Simon MN, Studier FW. Analysis of restriction fragments of T7 DNA and determination of molecular weights by electrophoresis in neutral and alkaline gels. J Mol Biol. 1977 Feb 15;110(1):119–146. [PubMed]
  • Nathans D, Smith HO. Restriction endonucleases in the analysis and restructuring of dna molecules. Annu Rev Biochem. 1975;44:273–293. [PubMed]
  • Rochaix JD. Restriction endonuclease map of the chloroplast DNA of Chlamydomonas reinhardii. J Mol Biol. 1978 Dec 25;126(4):597–617. [PubMed]
  • Malnoë P, Rochaix JD, Chua NH, Spahr PF. Characterization of the gene and messenger RNA of the large subunit of ribulose 1,5-diphosphate carboxylase in Chlamydomonas reinhardii. J Mol Biol. 1979 Sep 25;133(3):417–434. [PubMed]
  • Wiseman A, Gillham NW, Boynton JE. The mitochondrial genome of Chlamydomonas. II. Genetic analysis of non-mendelian obligate photautotrophic mutants. Mol Gen Genet. 1977 Jan 18;150(2):109–118. [PubMed]
  • Pring DR, Levings CS. Heterogeneity of Maize Cytoplasmic Genomes among Male-Sterile Cytoplasms. Genetics. 1978 May;89(1):121–136. [PMC free article] [PubMed]
  • Buzzo K, Fouts DL, Wolstenholme DR. EcoRI cleavage site variants of mitochondrial DNA molecules from rats. Proc Natl Acad Sci U S A. 1978 Feb;75(2):909–913. [PMC free article] [PubMed]
  • Francisco JF, Brown GG, Simpson MV. Further studies on types A and B rat mtDNAs: cleavage maps and evidence for cytoplasmic inheritance in mammals. Plasmid. 1979 Jul;2(3):426–436. [PubMed]
  • Hayashi JI, Yonekawa H, Gotoh O, Watanabe J, Tagashira Y. Strictly maternal inheritance of rat mitochondrial DNA. Biochem Biophys Res Commun. 1978 Aug 14;83(3):1032–1038. [PubMed]
  • Kroon AM, de Vos WM, Bakker H. The heterogeneity of rat-liver mitochondrial DNA. Biochim Biophys Acta. 1978 Jun 22;519(1):269–273. [PubMed]
  • Mannella CA, Pittenger TH, Lambowitz AM. Transmission of mitochondrial deoxyribonucleic acid in Neurospora crassa sexual crosses. J Bacteriol. 1979 Mar;137(3):1449–1451. [PMC free article] [PubMed]
  • Levings CS, 3rd, Pring DR. Restriction endonuclease analysis of mitochondrial DNA from normal and Texas cytoplasmic male-sterile maize. Science. 1976 Jul 9;193(4248):158–160. [PubMed]
  • Hutchison CA, 3rd, Newbold JE, Potter SS, Edgell MH. Maternal inheritance of mammalian mitochondrial DNA. Nature. 1974 Oct 11;251(5475):536–538. [PubMed]
  • Reilly JG, Thomas CA., Jr Length polymorphisms, restriction site variation, and maternal inheritance of mitochondrial DNA of Drosophila melanogaster. Plasmid. 1980 Mar;3(2):109–115. [PubMed]
  • Avise JC, Lansman RA, Shade RO. The use of restriction endonucleases to measure mitochondrial DNA sequence relatedness in natural populations. I. Population structure and evolution in the genus Peromyscus. Genetics. 1979 May;92(1):279–295. [PMC free article] [PubMed]
  • Brown WM, Wright JW. Mitochondrial DNA analyses and the origin and relative age of parthenogenetic lizards (genus Cnemidophorus). Science. 1979 Mar 23;203(4386):1247–1249. [PubMed]

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