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Proc Natl Acad Sci U S A. Oct 1980; 77(10): 5716–5719.
PMCID: PMC350140

NH2-terminal amino acid sequence of human fibroblast interferon.


The purification of human fibroblast interferon by chromatography on Blue Sepharose and high-performance liquid chromatography is described. The amino acid composition and a partial sequence of the homogeneous protein are reported. The NH2 terminus was determined to be NH2-Met1-Ser-Tyr-Asn-Leu-Leu-Gly-Phe-Leu-Gln-Arg-Ser-Ser-Asn-Phe-Gln-X-Gln-Lys.

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Selected References

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  • Knight E, Jr, Hunkapiller MW, Korant BD, Hardy RW, Hood LE. Human fibroblast interferon: amino acid analysis and amino terminal amino acid sequence. Science. 1980 Feb 1;207(4430):525–526. [PubMed]
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  • Tan YH, Barakat F, Berthold W, Smith-Johannsen H, Tan C. The isolation and amino acid/sugar composition of human fibroblastoid interferon. J Biol Chem. 1979 Aug 25;254(16):8067–8073. [PubMed]
  • Levy WP, Shively J, Rubinstein M, Del Valle U, Pestka S. Amino-terminal amino acid sequence of human leukocyte interferon. Proc Natl Acad Sci U S A. 1980 Sep;77(9):5102–5104. [PMC free article] [PubMed]
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