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Proc Natl Acad Sci U S A. Oct 1980; 77(10): 5716–5719.
PMCID: PMC350140

NH2-terminal amino acid sequence of human fibroblast interferon.

Abstract

The purification of human fibroblast interferon by chromatography on Blue Sepharose and high-performance liquid chromatography is described. The amino acid composition and a partial sequence of the homogeneous protein are reported. The NH2 terminus was determined to be NH2-Met1-Ser-Tyr-Asn-Leu-Leu-Gly-Phe-Leu-Gln-Arg-Ser-Ser-Asn-Phe-Gln-X-Gln-Lys.

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Selected References

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  • Knight E, Jr, Hunkapiller MW, Korant BD, Hardy RW, Hood LE. Human fibroblast interferon: amino acid analysis and amino terminal amino acid sequence. Science. 1980 Feb 1;207(4430):525–526. [PubMed]
  • Berthold W, Tan C, Tan YH. Purification and in vitro labeling of interferon from a human fibroblastoid cell line. J Biol Chem. 1978 Jul 25;253(14):5206–5212. [PubMed]
  • Havell EA, Vilcek J. Production of high-titered interferon in cultures of human diploid cells. Antimicrob Agents Chemother. 1972 Dec;2(6):476–484. [PMC free article] [PubMed]
  • Jankowski WJ, von Muenchhausen W, Sulkowski E, Carter WA. Binding of human interferons to immobolized Cibacron Blue F3GA: The nature of molecular interaction. Biochemistry. 1976 Nov 16;15(23):5182–5187. [PubMed]
  • Rubinstein M, Rubinstein S, Familletti PC, Miller RS, Waldman AA, Pestka S. Human leukocyte interferon: production, purification to homogeneity, and initial characterization. Proc Natl Acad Sci U S A. 1979 Feb;76(2):640–644. [PMC free article] [PubMed]
  • Tucker P, Pestka S. De novo synthesis and glycosylation of the MOPC-46B mouse immunoglobulin light chain in cell-free extracts. J Biol Chem. 1977 Jul 10;252(13):4474–4486. [PubMed]
  • Stein S, Böhlen P, Stone J, Dairman W, Udenfriend S. Amino acid analysis with fluorescamine at the picomole level. Arch Biochem Biophys. 1973 Mar;155(1):202–212. [PubMed]
  • Böhlen P, Stein S, Stone J, Udenfriend S. Automatic Monitoring of primary amines in preparative column effluents with fluorescamine. Anal Biochem. 1975 Aug;67(2):438–445. [PubMed]
  • Wittmann-Liebold B. Amino acid sequence studies on ten ribosomal proteins of Escherichia coli with an improved sequenator equipped with an automatic conversion device. Hoppe Seylers Z Physiol Chem. 1973 Oct-Nov;354(10-11):1415–1431. [PubMed]
  • Hunkapiller MW, Hood LE. Direct microsequence analysis of polypeptides using an improved sequenator, a nonprotein carrier (polybrene), and high pressure liquid chromatography. Biochemistry. 1978 May 30;17(11):2124–2133. [PubMed]
  • Wittmann-Liebold B, Graffunder H, Kohls H. A device coupled to a modified sequenator for the automated conversion of anilinothiazolinones into PTH amino acids. Anal Biochem. 1976 Oct;75(2):621–633. [PubMed]
  • Taira H, Broeze RJ, Jayaram BM, Lengyel P, Hunkapiller MW, Hood LE. Mouse interferons: amino terminal amino acid sequences of various species. Science. 1980 Feb 1;207(4430):528–530. [PubMed]
  • Zoon KC, Smith ME, Bridgen PJ, Anfinsen CB, Hunkapiller MW, Hood LE. Amino terminal sequence of the major component of human lymphoblastoid interferon. Science. 1980 Feb 1;207(4430):527–528. [PubMed]
  • Tan YH, Barakat F, Berthold W, Smith-Johannsen H, Tan C. The isolation and amino acid/sugar composition of human fibroblastoid interferon. J Biol Chem. 1979 Aug 25;254(16):8067–8073. [PubMed]
  • Levy WP, Shively J, Rubinstein M, Del Valle U, Pestka S. Amino-terminal amino acid sequence of human leukocyte interferon. Proc Natl Acad Sci U S A. 1980 Sep;77(9):5102–5104. [PMC free article] [PubMed]
  • Cavalieri RL, Havell EA, Vilcek J, Pestka S. Synthesis of human interferon by Xenopus laevis oocytes: two structural genes for interferons in human cells. Proc Natl Acad Sci U S A. 1977 Aug;74(8):3287–3291. [PMC free article] [PubMed]

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