• We are sorry, but NCBI web applications do not support your browser and may not function properly. More information
Logo of pnasPNASInfo for AuthorsSubscriptionsAboutThis Article
Proc Natl Acad Sci U S A. Sep 1980; 77(9): 5390–5393.
PMCID: PMC350064

Four heat shock proteins of Drosophila melanogaster coded within a 12-kilobase region in chromosome subdivision 67B.

Abstract

Unique coding sequences for four heat shock proteins of Drosophila melanogaster, hsp 28, hsp 26, hsp 23, and hsp 22, are clustered in a 12-kilobase interval at chromosome subdivision 67B. The four genes are not transcribed in the same direction and each gives rise to a separate messenger RNA, with no indication of intervening sequences. Including the present results, the genes for all seven major heat shock proteins of D. melanogaster are now cloned are found to exhibit a variety of patterns of organization at the five loci they occupy.

Full text

Full text is available as a scanned copy of the original print version. Get a printable copy (PDF file) of the complete article (1.1M), or click on a page image below to browse page by page. Links to PubMed are also available for Selected References.

Images in this article

Click on the image to see a larger version.

Selected References

These references are in PubMed. This may not be the complete list of references from this article.
  • Ashburner M, Bonner JJ. The induction of gene activity in drosophilia by heat shock. Cell. 1979 Jun;17(2):241–254. [PubMed]
  • Holmgren R, Livak K, Morimoto R, Freund R, Meselson M. Studies of cloned sequences from four Drosophila heat shock loci. Cell. 1979 Dec;18(4):1359–1370. [PubMed]
  • Ish-Horowicz D, Pinchin SM, Schedl P, Artavanis-Tsakonas S, Mirault ME. Genetic and molecular analysis of the 87A7 and 87C1 heat-inducible loci of D. melanogaster. Cell. 1979 Dec;18(4):1351–1358. [PubMed]
  • Wadsworth SC, Craig EA, McCarthy BJ. Genes for three Drosophila heat-shock-induced proteins at a single locus. Proc Natl Acad Sci U S A. 1980 Apr;77(4):2134–2137. [PMC free article] [PubMed]
  • Peterson NS, Moller G, Mitchell HK. Genetic mapping of the coding regions for three heat-shock proteins in Drosophila melanogaster. Genetics. 1979 Jul;92(3):891–902. [PMC free article] [PubMed]
  • McKenzie SL, Meselson M. Translation in vitro of Drosophila heat-shock messages. J Mol Biol. 1977 Nov 25;117(1):279–283. [PubMed]
  • Livak KJ, Freund R, Schweber M, Wensink PC, Meselson M. Sequence organization and transcription at two heat shock loci in Drosophila. Proc Natl Acad Sci U S A. 1978 Nov;75(11):5613–5617. [PMC free article] [PubMed]
  • Maniatis T, Hardison RC, Lacy E, Lauer J, O'Connell C, Quon D, Sim GK, Efstratiadis A. The isolation of structural genes from libraries of eucaryotic DNA. Cell. 1978 Oct;15(2):687–701. [PubMed]
  • Zimmerman SB, Sandeen D. The ribonuclease activity of crystallized pancreatic deoxyribonuclease. Anal Biochem. 1966 Feb;14(2):269–277. [PubMed]
  • Donelson JE, Wu R. Nucleotide sequence analysis of deoxyribonucleic acid. VII. Characterization of Escherichia coli exonuclease 3 activity for possible use in terminal nucleotide sequence analysis of duplex deoxyribonucleic acid. J Biol Chem. 1972 Jul 25;247(14):4661–4668. [PubMed]
  • Maxam AM, Gilbert W. Sequencing end-labeled DNA with base-specific chemical cleavages. Methods Enzymol. 1980;65(1):499–560. [PubMed]
  • Efstratiadis A, Kafatos FC, Maxam AM, Maniatis T. Enzymatic in vitro synthesis of globin genes. Cell. 1976 Feb;7(2):279–288. [PubMed]
  • Mirault ME, Goldschmidt-Clermont M, Moran L, Arrigo AP, Tissières A. The effect of heat shock on gene expression in Drosophila melanogaster. Cold Spring Harb Symp Quant Biol. 1978;42(Pt 2):819–827. [PubMed]
  • Berk AJ, Sharp PA. Spliced early mRNAs of simian virus 40. Proc Natl Acad Sci U S A. 1978 Mar;75(3):1274–1278. [PMC free article] [PubMed]
  • Kaback DB, Angerer LM, Davidson N. Improved methods for the formation and stabilization of R-loops. Nucleic Acids Res. 1979 Jun 11;6(7):2499–2317. [PMC free article] [PubMed]
  • Ricciardi RP, Miller JS, Roberts BE. Purification and mapping of specific mRNAs by hybridization-selection and cell-free translation. Proc Natl Acad Sci U S A. 1979 Oct;76(10):4927–4931. [PMC free article] [PubMed]
  • O'Farrell PH. High resolution two-dimensional electrophoresis of proteins. J Biol Chem. 1975 May 25;250(10):4007–4021. [PMC free article] [PubMed]
  • Paterson BM, Roberts BE, Kuff EL. Structural gene identification and mapping by DNA-mRNA hybrid-arrested cell-free translation. Proc Natl Acad Sci U S A. 1977 Oct;74(10):4370–4374. [PMC free article] [PubMed]
  • Sutcliffe JG. pBR322 restriction map derived from the DNA sequence: accurate DNA size markers up to 4361 nucleotide pairs long. Nucleic Acids Res. 1978 Aug;5(8):2721–2728. [PMC free article] [PubMed]

Articles from Proceedings of the National Academy of Sciences of the United States of America are provided here courtesy of National Academy of Sciences

Formats:

Related citations in PubMed

See reviews...See all...

Cited by other articles in PMC

See all...

Links

Recent Activity

Your browsing activity is empty.

Activity recording is turned off.

Turn recording back on

See more...