• We are sorry, but NCBI web applications do not support your browser and may not function properly. More information
Logo of pnasPNASInfo for AuthorsSubscriptionsAboutThis Article
Proc Natl Acad Sci U S A. Jun 1980; 77(6): 3595–3599.
PMCID: PMC349664

Comparative gene mapping: order of loci on the X chromosome is different in mice and humans.

Abstract

For comparative studies we have used the somatic cell hybridization approach to regionally map genes on the mouse X chromosome. Fibroblasts from a mouse with the balanced reciprocal translocation T(XD;16B5)16H were fused with a Chinese hamster cell line (V79/380-6) deficient in activity of the enzyme hypoxanthine phosphoribosyltransferase (HPRT). Interpecific cell hybrids were initially selected for retention of the mouse translocation chromosome carrying the Hprt gene. Subsequently, hybrid clones were counterselected to force segregation of this chromosome. Selected and counterselected hybrid clones were analyzed for their chromosome content by trypsin/Giemsa banding and for expression of the mouse forms of the X-linked enzymes HPRT and alpha-galactosidase (GALA) by isoelectric focusing. The results indicate that the breakpoint on the mouse X chromosome (in band XD) has separated the genes for HPRT (Hprt) and for GALA (Ags). Hprt is proximal to the breakpoint in region Xcen-XD and Ags is distal in region XD-Xter. The gene order in the mouse (centromere-Hprt-Ags) is therefore inverted when compared to the order of the homologous loci on the long arm of the human X (centromere-GALA-HPRT).

Full text

Full text is available as a scanned copy of the original print version. Get a printable copy (PDF file) of the complete article (1.5M), or click on a page image below to browse page by page. Links to PubMed are also available for Selected References.

Images in this article

Selected References

These references are in PubMed. This may not be the complete list of references from this article.
  • Ono S. Ancient linkage groups and frozen accidents. Nature. 1973 Aug 3;244(5414):259–262. [PubMed]
  • Pearson PL, Roderick TH, Davisson MT, Garver JJ, Warburton D, Lalley PA, O'Brien SJ. Report of the committee on comparative mapping. Cytogenet Cell Genet. 1979;25(1-4):82–95. [PubMed]
  • Kozak C, Nichols E, Ruddle FH. Gene linkage analysis in the mouse by somatic cell hybridization: assignment of adenine phosphoribosyltransferase to chromosome 8 and alpha-galactosidase to the X chromosome. Somatic Cell Genet. 1975 Oct;1(4):371–382. [PubMed]
  • Francke U, Lalley PA, Moss W, Ivy J, Minna JD. Gene mapping in Mus musculus by interspecific cell hybridization: assignment of the genes for tripeptidase-1 to chromosome 10, dipeptidase-2 to chromosome 18, acid phosphatase-1 to chromosome 12, and adenylate kinase-1 to chromosome 2. Cytogenet Cell Genet. 1977;19(2-3):57–84. [PubMed]
  • Chapman VM, Shows TB. Somatic cell genetic evidence for X-chromosome linkage of three enzymes in the mouse. Nature. 1976 Feb 26;259(5545):665–667. [PubMed]
  • Hashimi S, Miller OJ. Further evidence of X-linkage of hypoxanthine phosphoribosyl-transferase in the mouse. Cytogenet Cell Genet. 1976;17(1):35–41. [PubMed]
  • Kozak LP, McLean GK, Eicher EM. X linkage of phosphoglycerate kinase in the mouse. Biochem Genet. 1974 Jan;11(1):41–47. [PubMed]
  • Nielsen JT, Chapman VM. Electrophoretic variation for x-chromosome-linked phosphoglycerate kinase (pgk-1) in the mouse. Genetics. 1977 Oct;87(2):319–325. [PMC free article] [PubMed]
  • Lusis AJ, West JD. X-linked inheritance of a structural gene for alpha-galactosidase in Mus musculus. Biochem Genet. 1976 Oct;14(9-10):849–855. [PubMed]
  • Epstein CJ. Mammalian oocytes: X chromosome activity. Science. 1969 Mar 7;163(3871):1078–1079. [PubMed]
  • Epstein CJ. Expression of the mammalian X chromosome before and after fertilization. Science. 1972 Mar 31;175(4029):1467–1468. [PubMed]
  • 4th International Workshop on Human Gene Mapping. Report of the committee on the genetic constitution of the X and Y chromosomes. Cytogenet Cell Genet. 1978;22(1-6):124–128. [PubMed]
  • LYON MF, SEARLE AG, FORD CE, OHNO S. A MOUSE TRANSLOCATION SUPPRESSING SEX-LINKED VARIEGATION. Cytogenetics. 1964;3:306–323. [PubMed]
  • Lyon MF. Order of loci on the X-chromosome of the mouse. Genet Res. 1966 Feb;7(1):130–133. [PubMed]
  • Eicher EM, Nesbitt MN, Francke U. Cytological identification of the chromosomes involved in Searle's translocation and the location of the centromere in the X chromosome of the mouse. Genetics. 1972 Aug;71(4):643–648. [PMC free article] [PubMed]
  • Francke U, Taggart RT. Assignment of the gene for cytoplasmic superoxide dismutase (Sod-1) to a region of chromosome 16 and of Hprt to a region of the X chromosome in the mouse. Proc Natl Acad Sci U S A. 1979 Oct;76(10):5230–5233. [PMC free article] [PubMed]
  • LITTLEFIELD JW. SELECTION OF HYBRIDS FROM MATINGS OF FIBROBLASTS IN VITRO AND THEIR PRESUMED RECOMBINANTS. Science. 1964 Aug 14;145(3633):709–710. [PubMed]
  • Francke U, Oliver N. Quantitative analysis of high-resolution trypsin-giemsa bands on human prometaphase chromosomes. Hum Genet. 1978 Dec 18;45(2):137–165. [PubMed]
  • Nesbitt MN, Francke U. A system of nomenclature for band patterns of mouse chromosomes. Chromosoma. 1973;41(2):145–158. [PubMed]
  • Searle AG, Beechey CV, Eicher EM, Nesbitt MN, Washburn LL. Colinearity in the mouse genome: a study of chromosome 2. Cytogenet Cell Genet. 1979;23(4):255–263. [PubMed]
  • Lyon MF. Distribution of crossing-over in mouse chromosomes. Genet Res. 1976 Dec;28(3):291–299. [PubMed]

Articles from Proceedings of the National Academy of Sciences of the United States of America are provided here courtesy of National Academy of Sciences

Formats:

Related citations in PubMed

See reviews...See all...

Cited by other articles in PMC

See all...

Links

  • Gene
    Gene
    Gene links
  • GEO Profiles
    GEO Profiles
    Related GEO records
  • HomoloGene
    HomoloGene
    HomoloGene links
  • MedGen
    MedGen
    Related information in MedGen
  • Pathways + GO
    Pathways + GO
    Pathways, annotations and biological systems (BioSystems) that cite the current article.
  • PubMed
    PubMed
    PubMed citations for these articles
  • Substance
    Substance
    PubChem Substance links

Recent Activity

Your browsing activity is empty.

Activity recording is turned off.

Turn recording back on

See more...