• We are sorry, but NCBI web applications do not support your browser and may not function properly. More information
Logo of pnasPNASInfo for AuthorsSubscriptionsAboutThis Article
Proc Natl Acad Sci U S A. Mar 1980; 77(3): 1365–1368.
PMCID: PMC348495

ATP-dependent conjugation of reticulocyte proteins with the polypeptide required for protein degradation.

Abstract

The heat-stable polypeptide (APF-1) required for ATP-dependent proteolysis in reticulocytes enters into high molecular weight conjugates upon incubation with the fraction of reticulocytes that is retained by DEAE-cellulose. Conjugate formation requires ATP and Mg2+ and its inhibited by N-ethylmaleimide. UTP and GTP are inactive. These properties are identical to those of ATP-dependent protein breakdown in the same system, suggesting that the conjugates are intermediates in this process. The APF-1 conjugates are stable in sodium dodecyl sulfate/polyacrylamide gel electrophoresis and Sephadex G-75 isolation and are resistant to mild acid, alkali, heat denaturation, and reduction; the conjugates are therefore covalent.

Full text

Full text is available as a scanned copy of the original print version. Get a printable copy (PDF file) of the complete article (894K), or click on a page image below to browse page by page. Links to PubMed are also available for Selected References.

Images in this article

Click on the image to see a larger version.

Selected References

These references are in PubMed. This may not be the complete list of references from this article.
  • SIMPSON MV. The release of labeled amino acids from the proteins of rat liver slices. J Biol Chem. 1953 Mar;201(1):143–154. [PubMed]
  • STEINBERG D, VAUGHAN M. Observations on intracellular protein catabolism studied in vitro. Arch Biochem Biophys. 1956 Nov;65(1):93–105. [PubMed]
  • Hershko A, Tomkins GM. Studies on the degradation of tyrosine aminotransferase in hepatoma cells in culture. Influence of the composition of the medium and adenosine triphosphate dependence. J Biol Chem. 1971 Feb 10;246(3):710–714. [PubMed]
  • MANDELSTAM J. Turnover of protein in growing and non-growing populations of Escherichia coli. Biochem J. 1958 May;69(1):110–119. [PMC free article] [PubMed]
  • Shechter Y, Rafaeli-Eshkol D, Hershko A. Influence of protease inhibitors and energy metabolism on intracellular protein breakdown in starving Escherichia coli. Biochem Biophys Res Commun. 1973 Oct 15;54(4):1518–1524. [PubMed]
  • Poole B, Wibo M. Protein degradation in cultured cells. The effect of fresh medium, fluoride, and iodoacetate on the digestion of cellular protein of rat fibroblasts. J Biol Chem. 1973 Sep 10;248(17):6221–6226. [PubMed]
  • Epstein D, Elias-Bishko S, Hershko A. Requirement for protein synthesis in the regulation of protein breakdown in cultured hepatoma cells. Biochemistry. 1975 Nov 18;14(23):5199–5204. [PubMed]
  • Pine MJ. Turnover of intracellular proteins. Annu Rev Microbiol. 1972;26:103–126. [PubMed]
  • Kowit JD, Goldberg AL. Intermediate steps in the degradation of a specific abnormal protein in Escherichia coli. J Biol Chem. 1977 Dec 10;252(23):8350–8357. [PubMed]
  • Etlinger JD, Goldberg AL. A soluble ATP-dependent proteolytic system responsible for the degradation of abnormal proteins in reticulocytes. Proc Natl Acad Sci U S A. 1977 Jan;74(1):54–58. [PMC free article] [PubMed]
  • Murakami K, Voellmy R, Goldberg AL. Protein degradation is stimulated by ATP in extracts of Escherichia coli. J Biol Chem. 1979 Sep 10;254(17):8194–8200. [PubMed]
  • Roberts JW, Roberts CW, Mount DW. Inactivation and proteolytic cleavage of phage lambda repressor in vitro in an ATP-dependent reaction. Proc Natl Acad Sci U S A. 1977 Jun;74(6):2283–2287. [PMC free article] [PubMed]
  • Roberts JW, Roberts CW, Craig NL. Escherichia coli recA gene product inactivates phage lambda repressor. Proc Natl Acad Sci U S A. 1978 Oct;75(10):4714–4718. [PMC free article] [PubMed]
  • Ciehanover A, Hod Y, Hershko A. A heat-stable polypeptide component of an ATP-dependent proteolytic system from reticulocytes. Biochem Biophys Res Commun. 1978 Apr 28;81(4):1100–1105. [PubMed]
  • Hershko A, Ciechanover A, Rose IA. Resolution of the ATP-dependent proteolytic system from reticulocytes: a component that interacts with ATP. Proc Natl Acad Sci U S A. 1979 Jul;76(7):3107–3110. [PMC free article] [PubMed]
  • Laemmli UK. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970 Aug 15;227(5259):680–685. [PubMed]

Articles from Proceedings of the National Academy of Sciences of the United States of America are provided here courtesy of National Academy of Sciences

Formats:

Related citations in PubMed

See reviews...See all...

Cited by other articles in PMC

See all...

Links

  • Compound
    Compound
    PubChem Compound links
  • MedGen
    MedGen
    Related information in MedGen
  • PubMed
    PubMed
    PubMed citations for these articles
  • Substance
    Substance
    PubChem Substance links

Recent Activity

Your browsing activity is empty.

Activity recording is turned off.

Turn recording back on

See more...