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Infect Immun. Jul 1982; 37(1): 151–154.
PMCID: PMC347503

Release of toxic microvesicles by Actinobacillus actinomycetemcomitans.

Abstract

Oral isolates of Actinobacillus actinomycetemcomitans (strain Y4) release spherical microvesicles in large numbers during normal growth. The biological activities of these products were studied, and it was estimated that approximately 1/10 of their dry weight was made up of heat- and proteolysis-resistant endotoxin. The chicken embryo lethality and bone-resorbing activity of the microvesicles were heat stable but proteolysis sensitive. Other laboratories have reported the presence of a heat- and proteolysis-sensitive leukotoxin in similar preparations. Accordingly, the microvesicles released by strain Y4 may contain, in addition to endotoxin, several potent substances which are highly toxic and active in bone resorption, and these may be significant factors in the pathogenesis of periodontal diseases.

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Selected References

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  • Baehni P, Tsai CC, McArthur WP, Hammond BF, Taichman NS. Interaction of inflammatory cells and oral microorganisms. VIII. Detection of leukotoxic activity of a plaque-derived gram-negative microorganism. Infect Immun. 1979 Apr;24(1):233–243. [PMC free article] [PubMed]
  • Devoe IW, Gilchrist JE. Release of endotoxin in the form of cell wall blebs during in vitro growth of Neisseria meningitidis. J Exp Med. 1973 Nov 1;138(5):1156–1167. [PMC free article] [PubMed]
  • Hausmann E, Raisz LG, Miller WA. Endotoxin: stimulation of bone resorption in tissue culture. Science. 1970 May 15;168(3933):862–864. [PubMed]
  • Horton JE, Raisz LG, Simmons HA, Oppenheim JJ, Mergenhagen SE. Bone resorbing activity in supernatant fluid from cultured human peripheral blood leukocytes. Science. 1972 Sep 1;177(4051):793–795. [PubMed]
  • Irving JT, Newman MG, Socransky SS, Heely JD. Histological changes in experimental periodontal disease in rats mono-infected with a gram-negative organism. Arch Oral Biol. 1975 Mar;20(3):219–220. [PubMed]
  • Lai CH, Listgarten MA, Hammond BF. Comparative ultrastructure of leukotoxic and non-leukotoxic strains of Actinobacillus actinomycetemcomitans. J Periodontal Res. 1981 Jul;16(4):379–389. [PubMed]
  • Lehrer S, Nowotny A. Isolation and purification of endotoxin by hydrolytic enzymes. Infect Immun. 1972 Dec;6(6):928–933. [PMC free article] [PubMed]
  • Listgarten MA, Lai CH. Comparative ultrastructure of Bacteroides melaninogenicus subspecies. J Periodontal Res. 1979 Jul;14(4):332–340. [PubMed]
  • Markham JL, Knox KW, Wicken AJ, Hewett MJ. Formation of extracellular lipoteichoic acid by oral streptococci and lactobacilli. Infect Immun. 1975 Aug;12(2):378–386. [PMC free article] [PubMed]
  • Marsh DG, Crutchley MJ. Purification and physico-chemical analysis of fractions from the culture supernatant of Escherichia coli O78K80: free endotoxin and a non-toxic fraction. J Gen Microbiol. 1967 Jun;47(3):405–420. [PubMed]
  • Newman MG, Socransky SS. Predominant cultivable microbiota in periodontosis. J Periodontal Res. 1977 Mar;12(2):120–128. [PubMed]
  • Poirier TP, Tonelli SJ, Holt SC. Ultrastructure of gliding bacteria: scanning electron microscopy of Capnocytophaga sputigena, Capnocytophaga gingivalis, and Capnocytophaga ochracea. Infect Immun. 1979 Dec;26(3):1146–1158. [PMC free article] [PubMed]
  • Raisz LG, Niemann I. Effect of phosphate, calcium and magnesium on bone resorption and hormonal responses in tissue culture. Endocrinology. 1969 Sep;85(3):446–452. [PubMed]
  • Robinson PJ, Shapiro IM. The effect of endotoxin and human dental plaque on the respiratory rate of bone cells. J Periodontal Res. 1975 Dec;10(6):305–308. [PubMed]
  • Slots J, Reynolds HS, Genco RJ. Actinobacillus actinomycetemcomitans in human periodontal disease: a cross-sectional microbiological investigation. Infect Immun. 1980 Sep;29(3):1013–1020. [PMC free article] [PubMed]
  • SMITH RT, THOMAS L. The lethal effect of endotoxins on the chick embryo. J Exp Med. 1956 Aug 1;104(2):217–231. [PMC free article] [PubMed]
  • Tripodi D, Nowotny A. Relation of structure to function in bacterial O-antigens. V. Nature of active sites in endotoxic lipopolysaccharides of Serratia marcescens. Ann N Y Acad Sci. 1966 Jun 30;133(2):604–621. [PubMed]
  • Work E, Knox KW, Vesk M. The chemistry and electron microscopy of an extracellular lipopolysaccharide from Escherichia coli. Ann N Y Acad Sci. 1966 Jun 30;133(2):438–449. [PubMed]

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