• We are sorry, but NCBI web applications do not support your browser and may not function properly. More information
Logo of pnasPNASInfo for AuthorsSubscriptionsAboutThis Article
Proc Natl Acad Sci U S A. Dec 1982; 79(24): 7809–7813.
PMCID: PMC347438

Chromosomal localization of human leukocyte, fibroblast, and immune interferon genes by means of in situ hybridization

Abstract

Recombinant plasmids containing cDNA inserts from human leukocyte interferon (IFN-α), fibroblast interferon (IFN-β), or immune interferon (INF-γ) genes were radiolabeled and hybridized in situ to human metaphase chromosome preparations. The results localized the human IFN-α and IFN-β genes to the short arm of chromosome 9(p21→pter) and localized the IFN-γ gene to the long arm of chromosome 12(q24.1).

Full text

Full text is available as a scanned copy of the original print version. Get a printable copy (PDF file) of the complete article (1.2M), or click on a page image below to browse page by page. Links to PubMed are also available for Selected References.

Images in this article

Click on the image to see a larger version.

Selected References

These references are in PubMed. This may not be the complete list of references from this article.
  • Goeddel DV, Yelverton E, Ullrich A, Heyneker HL, Miozzari G, Holmes W, Seeburg PH, Dull T, May L, Stebbing N, et al. Human leukocyte interferon produced by E. coli is biologically active. Nature. 1980 Oct 2;287(5781):411–416. [PubMed]
  • Goeddel DV, Leung DW, Dull TJ, Gross M, Lawn RM, McCandliss R, Seeburg PH, Ullrich A, Yelverton E, Gray PW. The structure of eight distinct cloned human leukocyte interferon cDNAs. Nature. 1981 Mar 5;290(5801):20–26. [PubMed]
  • Nagata S, Taira H, Hall A, Johnsrud L, Streuli M, Ecsödi J, Boll W, Cantell K, Weissmann C. Synthesis in E. coli of a polypeptide with human leukocyte interferon activity. Nature. 1980 Mar 27;284(5754):316–320. [PubMed]
  • Derynck R, Content J, DeClercq E, Volckaert G, Tavernier J, Devos R, Fiers W. Isolation and structure of a human fibroblast interferon gene. Nature. 1980 Jun 19;285(5766):542–547. [PubMed]
  • Goeddel DV, Shepard HM, Yelverton E, Leung D, Crea R, Sloma A, Pestka S. Synthesis of human fibroblast interferon by E. coli. Nucleic Acids Res. 1980 Sep 25;8(18):4057–4074. [PMC free article] [PubMed]
  • Gray PW, Leung DW, Pennica D, Yelverton E, Najarian R, Simonsen CC, Derynck R, Sherwood PJ, Wallace DM, Berger SL, et al. Expression of human immune interferon cDNA in E. coli and monkey cells. Nature. 1982 Feb 11;295(5849):503–508. [PubMed]
  • Sehgal PB, Sagar AD. Heterogeneity of poly(I) x poly(C)-induced human fibroblast interferon mRNA species. Nature. 1980 Nov 6;288(5786):95–97. [PubMed]
  • Weissenbach J, Chernajovsky Y, Zeevi M, Shulman L, Soreq H, Nir U, Wallach D, Perricaudet M, Tiollais P, Revel M. Two interferon mRNAs in human fibroblasts: in vitro translation and Escherichia coli cloning studies. Proc Natl Acad Sci U S A. 1980 Dec;77(12):7152–7156. [PMC free article] [PubMed]
  • Tan YH, Berthold W. A mechanism for the induction and regulation of human fibroblastoid interferon genetic expression. J Gen Virol. 1977 Mar;34(3):401–411. [PubMed]
  • Slate DL, Ruddle FH. Fibroblast interferon in man is coded by two loci on separate chromosomes. Cell. 1979 Jan;16(1):171–180. [PubMed]
  • Tan YH, Creagan RP, Ruddle FH. The somatic cell genetics of human interferon: assignment of human interferon loci to chromosomes 2 and 5. Proc Natl Acad Sci U S A. 1974 Jun;71(6):2251–2255. [PMC free article] [PubMed]
  • Meager A, Graves HE, Walker JR, Burke DC, Swallow DM, Westerveld A. Somatic cell genetics of human interferon production in human-rodent cell hybrids. J Gen Virol. 1979 Nov;45(2):309–321. [PubMed]
  • Tan YH, Schneider EL, Tischfield J, Epstein CJ, Ruddle FH. Human chromosome 21 dosage: effect on the expression of the interferon induced antiviral state. Science. 1974 Oct 4;186(4158):61–63. [PubMed]
  • Owerbach D, Rutter WJ, Martial JA, Baxter JD, Shows TB. Genes for growth hormone, chorionic somatommammotropin, and growth hormones-like gene on chromosome 17 in humans. Science. 1980 Jul 11;209(4453):289–292. [PubMed]
  • Owerbach D, Bell GI, Rutter WJ, Shows TB. The insulin gene is located on chromosome 11 in humans. Nature. 1980 Jul 3;286(5768):82–84. [PubMed]
  • Owerbach D, Rutter WJ, Roberts JL, Whitfeld P, Shine J, Seeburg PH, Shows TB. The proopiocortin (adrenocorticotropin/beta-lipoprotein) gene is located on chromosome 2 in humans. Somatic Cell Genet. 1981 May;7(3):359–369. [PubMed]
  • Owerbach D, Rutter WJ, Shows TB, Gray P, Goeddel DV, Lawn RM. Leukocyte and fibroblast interferon genes are located on human chromosome 9. Proc Natl Acad Sci U S A. 1981 May;78(5):3123–3127. [PMC free article] [PubMed]
  • Gusella JF, Keys C, VarsanyiBreiner A, Kao FT, Jones C, Puck TT, Housman D. Isolation and localization of DNA segments from specific human chromosomes. Proc Natl Acad Sci U S A. 1980 May;77(5):2829–2833. [PMC free article] [PubMed]
  • Harper ME, Saunders GF. Localization of single copy DNA sequences of G-banded human chromosomes by in situ hybridization. Chromosoma. 1981;83(3):431–439. [PubMed]
  • Harper ME, Barrera-Saldaña HA, Saunders GF. Chromosomal localization of the human placental lactogen-growth hormone gene cluster to 17q22-24. Am J Hum Genet. 1982 Mar;34(2):227–234. [PMC free article] [PubMed]
  • Gerhard DS, Kawasaki ES, Bancroft FC, Szabo P. Localization of a unique gene by direct hybridization in situ. Proc Natl Acad Sci U S A. 1981 Jun;78(6):3755–3759. [PMC free article] [PubMed]
  • Taylor JM, Illmensee R, Summers J. Efficeint transcription of RNA into DNA by avian sarcoma virus polymerase. Biochim Biophys Acta. 1976 Sep 6;442(3):324–330. [PubMed]
  • Wahl GM, Stern M, Stark GR. Efficient transfer of large DNA fragments from agarose gels to diazobenzyloxymethyl-paper and rapid hybridization by using dextran sulfate. Proc Natl Acad Sci U S A. 1979 Aug;76(8):3683–3687. [PMC free article] [PubMed]
  • Durie BG, Salmon SE. High speed scintillation autoradiography. Science. 1975 Dec 12;190(4219):1093–1095. [PubMed]
  • Chandler ME, Yunis JJ. A high resolution in situ hybridization technique for the direct visualization of labeled G-banded early metaphase and prophase chromosomes. Cytogenet Cell Genet. 1978;22(1-6):352–356. [PubMed]
  • Yunis JJ, Sawyer JR, Ball DW. Characterization of banding patterns of metaphase-prophase G-banded chromosomes and their use in gene mapping. Cytogenet Cell Genet. 1978;22(1-6):679–683. [PubMed]
  • Yunis JJ, Kuo MT, Saunders GF. Localization of sequences specifying messenger RNA to light-staining G-bands of human chromosomes. Chromosoma. 1977 Jun 23;61(4):335–344. [PubMed]
  • Yu LC, Szabo P, Borun TW, Prensky W. The localization of the genes coding for histone H4 in human chromosomes. Cold Spring Harb Symp Quant Biol. 1978;42(Pt 2):1101–1105. [PubMed]
  • Chandler ME, Kedes LH, Cohn RH, Yunis JJ. Genes coding for histone proteins in man are located on the distal end of the long arm of chromosome 7. Science. 1979 Aug 31;205(4409):908–910. [PubMed]
  • Nagata S, Mantei N, Weissmann C. The structure of one of the eight or more distinct chromosomal genes for human interferon-alpha. Nature. 1980 Oct 2;287(5781):401–408. [PubMed]
  • Lawn RM, Adelman J, Dull TJ, Gross M, Goeddel D, Ullrich A. DNA sequence of two closely linked human leukocyte interferon genes. Science. 1981 Jun 5;212(4499):1159–1162. [PubMed]
  • Nagata S, Brack C, Henco K, Schamböck A, Weissmann C. Partial mapping of ten genes of the human interferon- alpha family. J Interferon Res. 1981 Feb;1(2):333–336. [PubMed]
  • Brack C, Nagata S, Mantei N, Weissmann C. Molecular analysis of the human interferon-alpha gene family. Gene. 1981 Dec;15(4):379–394. [PubMed]
  • Ullrich A, Gray A, Goeddel DV, Dull TJ. Nucleotide sequence of a portion of human chromosome 9 containing a leukocyte interferon gene cluster. J Mol Biol. 1982 Apr 15;156(3):467–486. [PubMed]
  • Shows TB, Sakaguchi AY, Naylor SL, Goedell DV, Lawn RM. Clustering of leukocyte and fibroblast interferon genes of human chromosome 9. Science. 1982 Oct 22;218(4570):373–374. [PubMed]

Articles from Proceedings of the National Academy of Sciences of the United States of America are provided here courtesy of National Academy of Sciences

Formats:

Related citations in PubMed

See reviews...See all...

Cited by other articles in PMC

See all...

Links

  • Cited in Books
    Cited in Books
    PubMed Central articles cited in books
  • MedGen
    MedGen
    Related information in MedGen
  • OMIM
    OMIM
    OMIM record citing PubMed
  • PubMed
    PubMed
    PubMed citations for these articles
  • Substance
    Substance
    PubChem Substance links