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Journal List > Proc Natl Acad Sci U S A > v.79(20); Oct 1982

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Proc Natl Acad Sci U S A. 1982 October; 79(20): 6242–6245.
PMCID: PMC347096
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Expression of the alpha subunit of human chorionic gonadotropin is specifically correlated with tumorigenic expression in human cell hybrids.
E J Stanbridge, S W Rosen, and H H Sussman
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Abstract
The expression of HeLa parent phenotype protein markers, the alpha subunit of human chorionic gonadotropin and placental alkaline phosphatase isoenzymes, has been evaluated in paired tumorigenic and nontumorigenic HeLa-fibroblast human cell hybrids. Both of these proteins have been used clinically as markers of malignancy. The results showed that both are expressed in the hybrids. Expression of the gonadotropin subunit in the hybrids is specifically correlated with tumorigenicity; the placental alkaline phosphatase isoenzyme showed no such correlation and was expressed in both tumorigenic and nontumorigenic hybrids.
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Selected References
These references are in PubMed. This may not be the complete list of references from this article.
  • Gold P, Freedman SO. Specific carcinoembryonic antigens of the human digestive system. J Exp Med. 1965 Sep 1;122(3):467–481. [PubMed]
  • Vaitukaitis JL, Ross GT, Braunstein GD, Rayford PL. Gonadotropins and their subunits: basic and clinical studies. Recent Prog Horm Res. 1976;32:289–331. [PubMed]
  • Tashjian AH, Jr, Weintraub BD, Barowsky NJ, Rabson AS, Rosen SW. Subunits of human chorionic gonadotropin: unbalanced synthesis and secretion by clonal cell strains derived from a bronchogenic carcinoma. Proc Natl Acad Sci U S A. 1973 May;70(5):1419–1422. [PubMed]
  • Fishman WH. Perspectives on alkaline phosphatase isoenzymes. Am J Med. 1974 May;56(5):617–650. [PubMed]
  • Sussman HH, Small PA, Jr, Cotlove E. Human alkaline phosphatase. Immunochemical identification of organ-specific isoenzymes. J Biol Chem. 1968 Jan 10;243(1):160–166. [PubMed]
  • ARMITAGE P, DOLL R. The age distribution of cancer and a multi-stage theory of carcinogenesis. Br J Cancer. 1954 Mar;8(1):1–12. [PubMed]
  • Barrett JC, Ts'o PO. Evidence for the progressive nature of neoplastic transformation in vitro. Proc Natl Acad Sci U S A. 1978 Aug;75(8):3761–3765. [PubMed]
  • Comings DE. A general theory of carcinogenesis. Proc Natl Acad Sci U S A. 1973 Dec;70(12):3324–3328. [PubMed]
  • Rubin H. Is somatic mutation the major mechanism of malignant transformation? J Natl Cancer Inst. 1980 May;64(5):995–1000. [PubMed]
  • Stanbridge EJ. Suppression of malignancy in human cells. Nature. 1976 Mar 4;260(5546):17–20. [PubMed]
  • Stanbridge EJ, Wilkinson J. Analysis of malignancy in human cells: malignant and transformed phenotypes are under separate genetic control. Proc Natl Acad Sci U S A. 1978 Mar;75(3):1466–1469. [PubMed]
  • Stanbridge EJ, Flandermeyer RR, Daniels DW, Nelson-Rees WA. Specific chromosome loss associated with the expression of tumorigenicity in human cell hybrids. Somatic Cell Genet. 1981 Nov;7(6):699–712. [PubMed]
  • Stanbridge EJ, Wilkinson J. Dissociation of anchorage independence form tumorigenicity in human cell hybrids. Int J Cancer. 1980 Jul 15;26(1):1–8. [PubMed]
  • Der CJ, Stanbridge EJ. Alterations in the extracellular matrix organization associated with the reexpression of tumorigenicity in human cell hybrids. Int J Cancer. 1980 Oct 15;26(4):451–459. [PubMed]
  • Russell WC, Newman C, Williamson DH. A simple cytochemical technique for demonstration of DNA in cells infected with mycoplasmas and viruses. Nature. 1975 Feb 6;253(5491):461–462. [PubMed]
  • Stanbridge EJ, Ceredig R. Growth-regulatory control of human cell hybrids in nude mice. Cancer Res. 1981 Feb;41(2):573–580. [PubMed]
  • Bahl OP. Human chorionic gonadotropin. I. Purification and physicochemical properties. J Biol Chem. 1969 Feb 25;244(4):567–574. [PubMed]
  • Swaminathan N, Bahl OP. Dissociation and recombination of the subunits of human chorionic gonadotropin. Biochem Biophys Res Commun. 1970 Jul 27;40(2):422–427. [PubMed]
  • Mehdi SQ, Nussey SS. A radio-ligand receptor assay for the long-acting thyroid stimulator. Inhibition by the long-acting thyroid stimulator of the binding of radioiodinated thyroid-stimulating hormone to human thyroid membranes. Biochem J. 1975 Jan;145(1):105–111. [PubMed]
  • COX RP, MACLEOD CM. Alkaline phosphatase content and the effects of prednisolone on mammalian cells in culture. J Gen Physiol. 1962 Jan;45:439–485. [PubMed]
  • Griffin MJ, Price GH, Bazzell KL. A study of adenosine 3':5'-cyclic monophosphate, sodium butyrate and cortisol as inducers of HeLa alkaline phosphatase. Arch Biochem Biophys. 1974 Oct;164(2):619–623. [PubMed]
  • Koyama H, Ono T. Induction of alkaline phosphatase by 5-bromodeoxyuridine in a hybrid line between mouse and Chinese hamster in culture. Exp Cell Res. 1971 Dec;69(2):468–470. [PubMed]
  • Chou JY, Robinson JC, Wang SS. Effects of sodium butyrate on synthesis of human chorionic gonadotrophin in trophoblastic and non-trophoblastic tumours. Nature. 1977 Aug 11;268(5620):543–544. [PubMed]
  • Tralka TS, Rosen SW, Weintraub BD, Lieblich JM, Engel LW, Wetzel BK, Kingsbury EW, Rabson AS. Ultrastructural concomitants of sodium butyrate-enhanced ectopic production of chorionic gonadotropin and its alpha subunit human bronchogenic carcinoma (ChaGo) cells. J Natl Cancer Inst. 1979 Jan;62(1):45–61. [PubMed]
  • Chou JY, Wang SS, Robinson JC. Regulation of the synthesis of human chorionic gonadotrophin by 5-bromo-2'-deoxyuridine and dibutyryl cyclic AMP in trophoblastic and nontrophoblastic tumor cells. J Clin Endocrinol Metab. 1978 Jul;47(1):46–51. [PubMed]
  • Ludueña MA, Iverson GM, Sussman HH. Expression of liver and placental alkaline phosphatases in Chang liver cells. J Cell Physiol. 1977 Apr;91(1):119–129. [PubMed]
  • Hamilton TA, Wada HG, Sussman HH. Human placental cell surface antigens:expression by cultured cells of diverse phenotypic origin. J Supramol Struct. 1979;11(4):503–515. [PubMed]
  • Lieblich JM, Weintraub BD, Rosen SW, Chou JY, Robinson JC. HeLa cells secrete alpha subunit of glycoprotein tropic hormones. Nature. 1976 Apr 8;260(5551):530–532. [PubMed]
  • Hamilton TA, Tin AW, Sussman HH, Weintraub BD, Rosen SW. Independent expression of alpha-HCG and placental alkaline phosphatase in clonal human cell lines. Oncodev Biol Med. 1980;1(3):161–167. [PubMed]
  • Harris H, Miller OJ, Klein G, Worst P, Tachibana T. Suppression of malignancy by cell fusion. Nature. 1969 Jul 26;223(5204):363–368. [PubMed]
  • Sager R, Kovac PE. Genetic analysis of tumorigenesis: IV. Chromosome reduction and marker segregation in progeny clones from Chinese hamster cell hybrids. Somatic Cell Genet. 1979 Jul;5(4):491–502. [PubMed]
  • Kucherlapati R, Shin SI. Genetic control of tumorigenicity in interspecific mammalian cell hybrids. Cell. 1979 Mar;16(3):639–648. [PubMed]
  • Wilson PD, Benham F, Franks LM. Alkaline phosphatase phenotypes in tumour and non-tumour cell lines: not an invariable marker for neoplastic transformation. Cell Biol Int Rep. 1977 May;1(3):229–238. [PubMed]
  • Stanbridge EJ, Der CJ, Doersen CJ, Nishimi RY, Peehl DM, Weissman BE, Wilkinson JE. Human cell hybrids: analysis of transformation and tumorigenicity. Science. 1982 Jan 15;215(4530):252–259. [PubMed]
  • Lieblich JM, Rosen SW, Weintraub BD, Sindelar WF, Tralka TS, Rabson AS. Maintenance of ectopic chorionic gonadotropin and alpha subunit secretion by a human lung cancer cell line (ChaGo) transplanted into nude mice. J Natl Cancer Inst. 1977 Oct;59(4):1285–1289. [PubMed]
  • Ruddon RW, Bryan AH, Meade-Cobun KS, Pollack VA. Production of human chorionic gonadotropin and its subunits by human tumors growing in nude mice. Cancer Res. 1980 Nov;40(11):4007–4012. [PubMed]
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