• We are sorry, but NCBI web applications do not support your browser and may not function properly. More information
Logo of pnasPNASInfo for AuthorsSubscriptionsAboutThis Article
Proc Natl Acad Sci U S A. Jul 1982; 79(13): 3973–3977.
PMCID: PMC346558

Proteolytic processing of poliovirus polypeptides: antibodies to polypeptide P3-7c inhibit cleavage at glutamine-glycine pairs.

Abstract

Proteolytic processing of poliovirus polypeptides was examined by the addition of antibodies directed against the viral proteins P3-7c and P2-X to a cell-free translation extract prepared from infected HeLa cells. Antisera to P3-7c specifically inhibited in vitro processing at Gln-Gly pairs. Partial amino acid sequence analysis revealed a second Tyr-Gly pair that is utilized in protein processing. Neither Tyr-Gly cleavage is affected by antibody to P3-7c. Anti-P3-7c antibodies react not only with P3-7c but also with P3-6a and P3-2, two viral polypeptides NH2-coterminal with P3-7c. Preimmune and anti-P2-X antibodies had no effect on the processing of poliovirus proteins in vitro. We conclude that the activity responsible for processing poliovirus polypeptides at Gln-Gly pairs resides in the primary structure of P3-7c (or P3-2 and P3-6a) and not in P2-X.

Full text

Full text is available as a scanned copy of the original print version. Get a printable copy (PDF file) of the complete article (1.6M), or click on a page image below to browse page by page. Links to PubMed are also available for Selected References.

Images in this article

Click on the image to see a larger version.

Selected References

These references are in PubMed. This may not be the complete list of references from this article.
  • Kitamura N, Adler C, Wimmer E. Structure and expression of the picornavirus genome. Ann N Y Acad Sci. 1980;354:183–201. [PubMed]
  • Nomoto A, Kitamura N, Golini F, Wimmer E. The 5'-terminal structures of poliovirion RNA and poliovirus mRNA differ only in the genome-linked protein VPg. Proc Natl Acad Sci U S A. 1977 Dec;74(12):5345–5349. [PMC free article] [PubMed]
  • Pettersson RF, Flanegan JB, Rose JK, Baltimore D. 5'-Terminal nucleotide sequences of polio virus polyribosomal RNA and virion RNA are identical. Nature. 1977 Jul 21;268(5617):270–272. [PubMed]
  • Summers DF, Maizel JV., Jr Evidence for large precursor proteins in poliovirus synthesis. Proc Natl Acad Sci U S A. 1968 Mar;59(3):966–971. [PMC free article] [PubMed]
  • Holland JJ, Kiehn ED. Specific cleavage of viral proteins as steps in the synthesis and maturation of enteroviruses. Proc Natl Acad Sci U S A. 1968 Jul;60(3):1015–1022. [PMC free article] [PubMed]
  • Jacobson MF, Baltimore D. Polypeptide cleavages in the formation of poliovirus proteins. Proc Natl Acad Sci U S A. 1968 Sep;61(1):77–84. [PMC free article] [PubMed]
  • Kitamura N, Semler BL, Rothberg PG, Larsen GR, Adler CJ, Dorner AJ, Emini EA, Hanecak R, Lee JJ, van der Werf S, et al. Primary structure, gene organization and polypeptide expression of poliovirus RNA. Nature. 1981 Jun 18;291(5816):547–553. [PubMed]
  • Racaniello VR, Baltimore D. Molecular cloning of poliovirus cDNA and determination of the complete nucleotide sequence of the viral genome. Proc Natl Acad Sci U S A. 1981 Aug;78(8):4887–4891. [PMC free article] [PubMed]
  • Semler BL, Anderson CW, Kitamura N, Rothberg PG, Wishart WL, Wimmer E. Poliovirus replication proteins: RNA sequence encoding P3-1b and the sites of proteolytic processing. Proc Natl Acad Sci U S A. 1981 Jun;78(6):3464–3468. [PMC free article] [PubMed]
  • Semler BL, Hanecak R, Anderson CW, Wimmer E. Cleavage sites in the polypeptide precursors of poliovirus protein P2-X. Virology. 1981 Oct 30;114(2):589–594. [PubMed]
  • Larsen GR, Anderson CW, Dorner AJ, Semler BL, Wimmer E. Cleavage sites within the poliovirus capsid protein precursors. J Virol. 1982 Jan;41(1):340–344. [PMC free article] [PubMed]
  • Semler BL, Anderson CW, Hanecak R, Dorner LF, Wimmer E. A membrane-associated precursor to poliovirus VPg identified by immunoprecipitation with antibodies directed against a synthetic heptapeptide. Cell. 1982 Feb;28(2):405–412. [PubMed]
  • Emini EA, Elzinga M, Wimmer E. Carboxy-terminal analysis of poliovirus proteins: termination of poliovirus RNA translation and location of unique poliovirus polyprotein cleavage sites. J Virol. 1982 Apr;42(1):194–199. [PMC free article] [PubMed]
  • Korant B, Chow N, Lively M, Powers J. Virus-specified protease in poliovirus-infected HeLa cells. Proc Natl Acad Sci U S A. 1979 Jun;76(6):2992–2995. [PMC free article] [PubMed]
  • Palmenberg AC, Pallansch MA, Rueckert RR. Protease required for processing picornaviral coat protein resides in the viral replicase gene. J Virol. 1979 Dec;32(3):770–778. [PMC free article] [PubMed]
  • Gorbalenya AE, Svitkin YV, Kazachkov YA, Agol VI. Encephalomyocarditis virus-specific polypeptide p22 is involved in the processing of the viral precursor polypeptides. FEBS Lett. 1979 Dec 1;108(1):1–5. [PubMed]
  • Ziola BR, Scraba DG. Structure of the Mengo virion. IV. Amino- and carboxyl-terminal analyses of the major capsid polypeptides. Virology. 1976 May;71(1):111–121. [PubMed]
  • Laemmli UK. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970 Aug 15;227(5259):680–685. [PubMed]
  • Alwine JC, Kemp DJ, Stark GR. Method for detection of specific RNAs in agarose gels by transfer to diazobenzyloxymethyl-paper and hybridization with DNA probes. Proc Natl Acad Sci U S A. 1977 Dec;74(12):5350–5354. [PMC free article] [PubMed]
  • Symington J, Green M, Brackmann K. Immunoautoradiographic detection of proteins after electrophoretic transfer from gels to diazo-paper: analysis of adenovirus encoded proteins. Proc Natl Acad Sci U S A. 1981 Jan;78(1):177–181. [PMC free article] [PubMed]
  • Renart J, Reiser J, Stark GR. Transfer of proteins from gels to diazobenzyloxymethyl-paper and detection with antisera: a method for studying antibody specificity and antigen structure. Proc Natl Acad Sci U S A. 1979 Jul;76(7):3116–3120. [PMC free article] [PubMed]
  • LOWRY OH, ROSEBROUGH NJ, FARR AL, RANDALL RJ. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed]
  • Anderson CW, Lewis JB. Amino-terminal sequence of adenovirus type 2 proteins: hexon, fiber, component IX, and early protein 1B-15K. Virology. 1980 Jul 15;104(1):27–41. [PubMed]
  • Celma ML, Ehrenfeld E. Translation of poliovirus RNA in vitro: detection of two different initiation sites. J Mol Biol. 1975 Nov 15;98(4):761–780. [PubMed]
  • Pelham HR. Translation of encephalomyocarditis virus RNA in vitro yields an active proteolytic processing enzyme. Eur J Biochem. 1978 Apr 17;85(2):457–462. [PubMed]
  • Shih DS, Shih CT, Kew O, Pallansch M, Rueckert R, Kaesberg P. Cell-free synthesis and processing of the proteins of poliovirus. Proc Natl Acad Sci U S A. 1978 Dec;75(12):5807–5811. [PMC free article] [PubMed]
  • Shih DS, Shih CT, Zimmern D, Rueckert RR, Kaesberg P. Translation of encephalomyocarditis virus RNA in reticulocyte lysates: kinetic analysis of the formation of virion proteins and a protein required for processing. J Virol. 1979 May;30(2):472–480. [PMC free article] [PubMed]
  • Dorner AJ, Rothberg PG, Wimmer E. The fate of VPg during in vitro translation of poliovirus RNA. FEBS Lett. 1981 Sep 28;132(2):219–223. [PubMed]
  • Palmenberg AC, Rueckert RR. Evidence for intramolecular self-cleavage of picornaviral replicase precursors. J Virol. 1982 Jan;41(1):244–249. [PMC free article] [PubMed]

Articles from Proceedings of the National Academy of Sciences of the United States of America are provided here courtesy of National Academy of Sciences

Formats:

Related citations in PubMed

See reviews...See all...

Cited by other articles in PMC

See all...

Links

  • Compound
    Compound
    PubChem Compound links
  • MedGen
    MedGen
    Related information in MedGen
  • PubMed
    PubMed
    PubMed citations for these articles
  • Substance
    Substance
    PubChem Substance links

Recent Activity

Your browsing activity is empty.

Activity recording is turned off.

Turn recording back on

See more...