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Proc Natl Acad Sci U S A. Jul 1984; 81(14): 4255–4259.
PMCID: PMC345566

Nucleotide sequence of a growth-related mRNA encoding a member of the prolactin-growth hormone family.

Abstract

As part of the proliferative response to serum, mouse 3T3 cells produce a set of growth-related mRNAs identified by hybridization to cloned cDNAs. One of these mRNAs, which is about 1 kilobase long, appears within a few hours after stimulation of resting cells with serum or platelet-derived growth factor and reaches a high level during the transition from the G1 to the S phase of growth. This mRNA is translated in vitro into a protein of approximately 25 kilodaltons. The corresponding cloned cDNA of 791 base pairs has been sequenced; it contains a single open reading frame that encodes a protein of 224 amino acids with extensive sequence homology to mammalian prolactins. The initial 29-amino acid segment of the encoded protein resembles the signal sequences of prehormones. That the growth-related protein is not mouse prolactin is indicated by comparison of its predicted amino acid composition with that of mouse prolactin and by the distinct fragment patterns seen when restricted mouse DNA is probed with the cloned cDNA or rat prolactin cDNA. Therefore, the growth-related protein appears to be a new member of the prolactin-growth hormone family. Because of its relationship to prolactin and growth hormone and its association with cell proliferation, the protein has been called "proliferin."

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Selected References

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  • Pardee AB, Dubrow R, Hamlin JL, Kletzien RF. Animal cell cycle. Annu Rev Biochem. 1978;47:715–750. [PubMed]
  • Linzer DI, Nathans D. Growth-related changes in specific mRNAs of cultured mouse cells. Proc Natl Acad Sci U S A. 1983 Jul;80(14):4271–4275. [PMC free article] [PubMed]
  • Foster DN, Schmidt LJ, Hodgson CP, Moses HL, Getz MJ. Polyadenylylated RNA complementary to a mouse retrovirus-like multigene family is rapidly and specifically induced by epidermal growth factor stimulation of quiescent cells. Proc Natl Acad Sci U S A. 1982 Dec;79(23):7317–7321. [PMC free article] [PubMed]
  • Cochran BH, Reffel AC, Stiles CD. Molecular cloning of gene sequences regulated by platelet-derived growth factor. Cell. 1983 Jul;33(3):939–947. [PubMed]
  • Tu CP, Cohen SN. 3'-end labeling of DNA with [alpha-32P]cordycepin-5'-triphosphate. Gene. 1980 Jul;10(2):177–183. [PubMed]
  • Maxam AM, Gilbert W. Sequencing end-labeled DNA with base-specific chemical cleavages. Methods Enzymol. 1980;65(1):499–560. [PubMed]
  • Sanger F, Coulson AR. The use of thin acrylamide gels for DNA sequencing. FEBS Lett. 1978 Mar 1;87(1):107–110. [PubMed]
  • Hanahan D, Meselson M. Plasmid screening at high colony density. Gene. 1980 Jun;10(1):63–67. [PubMed]
  • Peden K, Mounts P, Hayward GS. Homology between mammalian cell DNA sequences and human herpesvirus genomes detected by a hybridization procedure with high-complexity probe. Cell. 1982 Nov;31(1):71–80. [PubMed]
  • Denhardt DT. A membrane-filter technique for the detection of complementary DNA. Biochem Biophys Res Commun. 1966 Jun 13;23(5):641–646. [PubMed]
  • Wallace RB, Johnson MJ, Hirose T, Miyake T, Kawashima EH, Itakura K. The use of synthetic oligonucleotides as hybridization probes. II. Hybridization of oligonucleotides of mixed sequence to rabbit beta-globin DNA. Nucleic Acids Res. 1981 Feb 25;9(4):879–894. [PMC free article] [PubMed]
  • Chirgwin JM, Przybyla AE, MacDonald RJ, Rutter WJ. Isolation of biologically active ribonucleic acid from sources enriched in ribonuclease. Biochemistry. 1979 Nov 27;18(24):5294–5299. [PubMed]
  • Glisin V, Crkvenjakov R, Byus C. Ribonucleic acid isolated by cesium chloride centrifugation. Biochemistry. 1974 Jun 4;13(12):2633–2637. [PubMed]
  • Aviv H, Leder P. Purification of biologically active globin messenger RNA by chromatography on oligothymidylic acid-cellulose. Proc Natl Acad Sci U S A. 1972 Jun;69(6):1408–1412. [PMC free article] [PubMed]
  • Gubbins EJ, Maurer RA, Hartley JL, Donelson JE. Construction and analysis of recombinant DNAs containing a structural gene for rat prolactin. Nucleic Acids Res. 1979 Mar;6(3):915–930. [PMC free article] [PubMed]
  • Gubbins EJ, Maurer RA, Lagrimini M, Erwin CR, Donelson JE. Structure of the rat prolactin gene. J Biol Chem. 1980 Sep 25;255(18):8655–8662. [PubMed]
  • Southern EM. Detection of specific sequences among DNA fragments separated by gel electrophoresis. J Mol Biol. 1975 Nov 5;98(3):503–517. [PubMed]
  • Rigby PW, Dieckmann M, Rhodes C, Berg P. Labeling deoxyribonucleic acid to high specific activity in vitro by nick translation with DNA polymerase I. J Mol Biol. 1977 Jun 15;113(1):237–251. [PubMed]
  • Laemmli UK. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970 Aug 15;227(5259):680–685. [PubMed]
  • Wilbur WJ, Lipman DJ. Rapid similarity searches of nucleic acid and protein data banks. Proc Natl Acad Sci U S A. 1983 Feb;80(3):726–730. [PMC free article] [PubMed]
  • Proudfoot NJ, Brownlee GG. Sequence at the 3' end of globin mRNA shows homology with immunoglobulin light chain mRNA. Nature. 1974 Nov 29;252(5482):359–362. [PubMed]
  • Efstratiadis A, Kafatos FC, Maniatis T. The primary structure of rabbit beta-globin mRNA as determined from cloned DNA. Cell. 1977 Apr;10(4):571–585. [PubMed]
  • Maurer RA. Transcriptional regulation of the prolactin gene by ergocryptine and cyclic AMP. Nature. 1981 Nov 5;294(5836):94–97. [PubMed]
  • Miller WL, Eberhardt NL. Structure and evolution of the growth hormone gene family. Endocr Rev. 1983 Spring;4(2):97–130. [PubMed]
  • Sasavage NL, Nilson JH, Horowitz S, Rottman FM. Nucleotide sequence of bovine prolactin messenger RNA. Evidence for sequence polymorphism. J Biol Chem. 1982 Jan 25;257(2):678–681. [PubMed]
  • von Heijne G. Patterns of amino acids near signal-sequence cleavage sites. Eur J Biochem. 1983 Jun 1;133(1):17–21. [PubMed]
  • Steiner DF, Quinn PS, Chan SJ, Marsh J, Tager HS. Processing mechanisms in the biosynthesis of proteins. Ann N Y Acad Sci. 1980;343:1–16. [PubMed]
  • Docherty K, Steiner DF. Post-translational proteolysis in polypeptide hormone biosynthesis. Annu Rev Physiol. 1982;44:625–638. [PubMed]
  • Colosi P, Talamantes F. The amino acid composition of secreted mouse prolactin, growth hormone, and hamster prolactin: the presence of one tryptophan in mouse prolactin. Arch Biochem Biophys. 1981 Dec;212(2):759–761. [PubMed]
  • Hendrickson SL, Scher CD. Platelet-derived growth factor-modulated translatable mRNAs. Mol Cell Biol. 1983 Aug;3(8):1478–1487. [PMC free article] [PubMed]
  • Kelly K, Cochran BH, Stiles CD, Leder P. Cell-specific regulation of the c-myc gene by lymphocyte mitogens and platelet-derived growth factor. Cell. 1983 Dec;35(3 Pt 2):603–610. [PubMed]
  • Lewis UJ, Singh RN, Lewis LJ, Seavey BK, Sinha YN. Glycosylated ovine prolactin. Proc Natl Acad Sci U S A. 1984 Jan;81(2):385–389. [PMC free article] [PubMed]
  • Colosi P, Marr G, Lopez J, Haro L, Ogren L, Talamantes F. Isolation, purification, and characterization of mouse placental lactogen. Proc Natl Acad Sci U S A. 1982 Feb;79(3):771–775. [PMC free article] [PubMed]
  • Golander A, Hurley T, Barrett J, Hizi A, Handwerger S. Prolactin synthesis by human chorion-decidual tissue: a possible source of prolactin in the amniotic fluid. Science. 1978 Oct 20;202(4365):311–313. [PubMed]
  • Riddick DH, Luciano AA, Kusmik WF, Maslar IA. De novo synthesis of prolactin by human decidua. Life Sci. 1978 Nov 9;23(19):1913–1921. [PubMed]
  • de Larco JE, Todaro GJ. Growth factors from murine sarcoma virus-transformed cells. Proc Natl Acad Sci U S A. 1978 Aug;75(8):4001–4005. [PMC free article] [PubMed]
  • D'Ercole AJ, Stiles AD, Underwood LE. Tissue concentrations of somatomedin C: further evidence for multiple sites of synthesis and paracrine or autocrine mechanisms of action. Proc Natl Acad Sci U S A. 1984 Feb;81(3):935–939. [PMC free article] [PubMed]
  • Kohmoto K, Tsunasawa S, Sakiyama F. Complete amino acid sequence of mouse prolactin. Eur J Biochem. 1984 Jan 16;138(2):227–237. [PubMed]

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