• We are sorry, but NCBI web applications do not support your browser and may not function properly. More information
Logo of jargspringer.comThis journalToc AlertsSubmit OnlineOpen Choice
J Assist Reprod Genet. Feb 2001; 18(2): 110–113.
PMCID: PMC3455561

PHYSIOLOGY: The Expression of Pseudogene Cyclin D2 mRNA in the Human Ovary May Be a Novel Marker for Decreased Ovarian Function Associated with the Aging Process

Abstract

Purpose: Our purpose was to investigate the expression pattern of cyclin D2 and pseudogene cyclin D2 mRNA in the human ovary with age.

Methods: After extraction of the total RNA from ovarian tissues of 23 premenopausal patients, cyclin D2 and pseudogene cyclin D2 mRNAs were measured by the reverse transcription–polymerase chain reaction technique using cyclin D2 and pseudogene cyclin D2 specific primers. Analysis of the cyclin D2 and pseudogene cyclin D2 mRNA expression pattern with age and correlation analysis were carried out.

Results: A 489-bp cyclin D2 band and a 441-bp pseudogene cycin D2 mRNA band were detected in the human ovarian tissue. While cyclin D2 mRNA expression showed a decreasing tendency with age (P = 0.17), pseudogene cyclin D2 mRNA expression increased with age (P < 0.05). Pseudogene cyclin D2 mRNA expression showed a negative correlation with cyclin D2 mRNA (R = −0.35, P < 0.03).

Conclusion: The expression of pseudogene cyclin D2 mRNA in the human ovary increases with age, which may be a novel marker for decreased ovarian function associated with the aging process.

Full Text

The Full Text of this article is available as a PDF (256K).

Selected References

These references are in PubMed. This may not be the complete list of references from this article.
1. Inaba T, Matsushime H, Valentine M, Roussel MF, Sherr CJ, Look AT. Genomic organization, chromosomal localization and independent expression of human cyclin D2 genes. Genomics. 1992;13:565–574. [PubMed]
2. Robker RL, Richards JS. Hormone-induced proliferation and differentiation of granulosa cells:Acoordinated balance of the cell cycle regulators cyclin D2 and p27kip1. Mol Endocrinol. 1998;12:924–940. [PubMed]
3. Sicinski P, Donaher JL, Geng Y, Parker SB, Gardner H, Park MY, et al. Cyclin D2 is a FSH-responsive gene involved in gonadal cell proliferation and oncogenesis. Nature. 1996;384:470–474. [PubMed]
4. Xiong Y, Menninger J, Beach D, Ward DC. Molecular cloning and chromosomal mapping of CCND genes encoding human D-type cyclins. Genomics. 1992;13:575–584. [PubMed]

Articles from Journal of Assisted Reproduction and Genetics are provided here courtesy of Springer Science+Business Media, LLC

Formats:

Related citations in PubMed

See reviews...See all...

Cited by other articles in PMC

See all...

Links

Recent Activity

Your browsing activity is empty.

Activity recording is turned off.

Turn recording back on

See more...