• We are sorry, but NCBI web applications do not support your browser and may not function properly. More information
Logo of ijmicrospringer.comThis journalToc AlertsSubmit OnlineOpen ChoiceThis journal
Indian J Microbiol. Mar 2010; 50(1): 76–82.
Published online Aug 27, 2010. doi:  10.1007/s12088-010-0048-3
PMCID: PMC3450283

Diversity and exploration of bioactive marine actinomycetes in the Bay of Bengal of the Puducherry coast of India

Abstract

The present study was designed to investigate the Puducherry coast of the Bay of Bengal, India for the diversity of bioactive actinomycetes. A total of 50 actinomycete strains were isolated from the marine sediments and most of the strains were belongs to Streptomyces. These strains were identified by means of morphological physiological, biochemical and cultural characteristics. The isolates were subjected to shake flask fermentation and the secondary metabolites were extracted with ethyl acetate and screened for cytotoxicity, hemolytic activity and antimicrobial activity against selected bacterial and fungal pathogens. The cytotoxic activity was evaluated using HeLa cell lines by 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide, a tetrazole (MTT) assay, hemolytic activity on mouse erythrocytes and the antifungal activity was evaluated by MTT cytotoxic assay against Aspergillus niger, Aspergillus fumigatus and Candida albicans. The antibacterial activity was studied against Staphylococcus aureus, Bacillus subtilis, Escherichia coli and Klebsiella pneumoniae. The cytotoxicity and antimicrobial activity of secondary metabolite was found to be concentration dependent and nearly 24% of isolates showed significant antimicrobial, hemolytic and cytotoxic activity. The results of our study indicate the diversity and bioactive potential of marine actinomycetes isolated in the Puducherry coast.

Full Text

The Full Text of this article is available as a PDF (313K).

Selected References

These references are in PubMed. This may not be the complete list of references from this article.
1. Bernan V.S., Greenstein M., Maiese W.M. Marine microorganisms as a source of new natural products. Adv Appl Microbiol. 1997;43:57–90. doi: 10.1016/S0065-2164(08)70223-5. [PubMed] [Cross Ref]
2. Singh S.B., Fernando P. Biodiversity, chemical diversity and drug discovery. Prog Drug Res. 2008;65:143–174. [PubMed]
3. Ismet A., Vikineswary S., Parameswari S., Wong W.H., Ward A., Seki T., Fiedler H.P., Goodfellow M. Production and chemical characterization of antifungal metabolites from Micromonospora species M39 isolated from mangrove rhizosphere soil. World J Microbiol Biotechnol. 2004;20:523–528. doi: 10.1023/B:WIBI.0000040399.60343.4c. [Cross Ref]
4. Cho K.W., Lee H.S., Rho J.R., Shin J. New lactone-containing metabolies from a marine derived bacterium of the genus Streptomyces. J Nat Prod. 2001;64:664–667. doi: 10.1021/np000599g. [PubMed] [Cross Ref]
5. Maskey R.P., Helmke E., Laatsch H. Himalomycin A and B: isolation and structure elucidation of new fridamycin type antibiotics from a marine Streptomyces isolate. J Antibiot. 2003;56:942–949. [PubMed]
6. Bredholdt H., Galatenko O.A., Engelhardt K. Rare actinomycete bacteria from the shallow water sediments of the Trondheim fjord, Norway: Isolation, diversity and biological activity. Environ Microbiol. 2007;43:2756–2764. doi: 10.1111/j.1462-2920.2007.01387.x. [PubMed] [Cross Ref]
7. Hopwood D.A. Therapeutic treasures from the deep. Nature Chem Biol. 2007;3:457–458. doi: 10.1038/nchembio0807-457. [PubMed] [Cross Ref]
8. Zengler K., Paradkar A., Keller M. New methods to access microbial diversity for small molecule discovery. In: Zhang L., Demain A.L., editors. Drug Discovery and Therapeutic Medicine from Natural Products. New Jersey, USA: Humana Press; 2005. pp. 275–293.
9. Sivakumar K., Sahu M.K., Thangaradjou T., Kannan L. Research on marine Actinobacteria in India. Indian J Microbiol. 2007;47:186–196. doi: 10.1007/s12088-007-0039-1. [PMC free article] [PubMed] [Cross Ref]
10. Sahu M.K., Sivakumar K., Kannan L. Isolation of marine actinomycetes inhibitory to human bacterial pathogens. Geobios. 2006;33:73–177.
11. Sivakumar K., Sahu M.K., Kathiresan K. Isolation and characterization of Streptomycetes, producing antibiotic, from a mangrove environment. Asian J Microbiol Biotech Env Sci. 2005;7:87–94.
12. Vijayakumar R., Muthukumar C., Thajuddin N., Panneerselvam A., Saravanamuthu R. Studies on the diversity of actinomycetes in the Palk Strait region of Bay of Bengal, India. Actinomycetologica. 2007;21:59–65. doi: 10.3209/saj.SAJ210203. [Cross Ref]
13. Ramesh S, Jayaprakashvel M and Mathivanan M (2006) Antimicrobial and extracellular enzymes producing actinomycetes from Bay of Bengal, India. 7th Asia Pacific Marine Biotechnology Conference, Kochi, India. Abstract S7-10.
14. Dhanasekaran D, Rajkumar G, Sivamani P, Selvamani S, Pannerselvam A and Thanjuddin N (2005) Screening of salt pans actinomycetes for antibacterial agents. Internet J Microbiol 1(2)
15. Shirling E.B., Gottlieb D. Methods for characterization of Streptomyces species. Int J Syst Bacteriol. 1966;16:312–340. doi: 10.1099/00207713-16-3-313. [Cross Ref]
16. Williams S.T., Goodfellow M., Alderson G. Genus Streptomyces. In: Williams S.T., editor. Bergey’s Manual of Systematic Bacteriology. Baltimore, USA: Williams & Wilkins Co.; 1989. pp. 2452–2492.
17. Pridham T.G. Colour and Streptomycetes. Report on International workshop on determination of color of Streptomycetes. Appl Microbiol. 1965;13:43–61. [PMC free article] [PubMed]
18. Hodgson V.J., Walker G.M., Button D. A rapid colorimetric assay of killer toxin activity in yeast FEMS. Microbiol Lett. 1994;120:201–205. doi: 10.1111/j.1574-6968.1994.tb07031.x. [PubMed] [Cross Ref]
19. Costa-Lotufo L.V., Cunha G.M.A., Farias P.A.M., Viana G.S.B., Cunha K.M.A., Pessoa C., Moraes M.O., Silveira E.R., Gramosa N.V., Rao V.S.N. The cytotoxic and embryotoxic effects of kaurenoic acid, a diterpene isolated from Copaifera langsdorffii oleo-resin. Toxicon. 2002;40:1231–1234. doi: 10.1016/S0041-0101(02)00128-9. [PubMed] [Cross Ref]
20. Jensen P.R., Dwight R., Fenical W. Distribution of actinomycetes in near-shore tropical marine sediments. App Env Microbiol. 1991;29:1102–1108. [PMC free article] [PubMed]
21. Peela S., Bapiraju K., Terli R. Studies on antagonistic marine actinomycetes from the Bay of Bengal. World J Microbiol Biotechnol. 2005;21:583–585. doi: 10.1007/s11274-004-3493-5. [Cross Ref]
22. Kokare C.R., Mahadik K.R., Kadam S.S., Chopade B.A. Isolation, characterisation and antimicrobial activity of marine halophilic Actinopolyspora species AH1 from the west coast of India. Curr Sci. 2003;86:594–597.

Articles from Indian Journal of Microbiology are provided here courtesy of Springer

Formats:

Related citations in PubMed

See reviews...See all...

Cited by other articles in PMC

Links

Recent Activity

Your browsing activity is empty.

Activity recording is turned off.

Turn recording back on

See more...