• We are sorry, but NCBI web applications do not support your browser and may not function properly. More information
Logo of jcmPermissionsJournals.ASM.orgJournalJCM ArticleJournal InfoAuthorsReviewers
J Clin Microbiol. Feb 2004; 42(2): 819–821.
PMCID: PMC344454

Simultaneous Presence of Multiple Campylobacter Species in Dogs


The prevalence of coinfection of Campylobacter species in dogs was determined using four isolation methods. In 26% of the positive-testing stools, multiple Campylobacter species were identified. The use of multiple isolation methods as well as the time lapse between sampling and processing are important for detection of coinfection.

In humans, Campylobacter is considered the most frequent bacterial cause of enteritis. The species Campylobacter jejuni is the prime etiologic agent, with minor contributions of Campylobacter coli and Campylobacter lari (1, 19). The role of Campylobacter as an enteric pathogen in dogs is much less evident. It is frequently isolated both from animals with symptoms of enteritis and from healthy animals (3, 9, 12, 16). The poor identification of Campylobacter species as animal pathogens may result from the simultaneous presence of multiple strains or species with various pathogenicity characteristics. In routine diagnostic laboratories, typing of Campylobacter is usually restricted to one colony per stool sample. In humans, simultaneous infection with more than one Campylobacter strain is found to be rare and is not considered to impair epidemiological analyses (15). With respect to dogs, however, no studies have been described that investigated the simultaneous presence of multiple species or strains. As the possibility to discriminate by colony morphology between Campylobacter species (13) or even between Campylobacter and Helicobacter species (6, 17) is limited, the prevalence of coinfection in companion animals could well be underestimated. Because the simultaneous presence of multiple strains or species is crucial to establish the role of Campylobacter in clinical disease as well as for epidemiological studies, we examined multiple colonies from a total of 30 fecal samples from diarrheic and nonsymptomatic dogs. We used a variety of culture media, as antimicrobials present in selective medium may selectively inhibit distinct Campylobacter species or strains. As Campylobacter is sensitive to oxygen and dryness, we also assessed the effect of the time interval between sampling and processing (transport time) on the isolation of Campylobacter.

Samples were obtained from September 2000 until February 2001 from household dogs of different ages (see Table Table2)2) and were transported to the laboratory without cooling. The average sample size was about 25 g (minimum, 5 g). The isolation methods employed included a filtration method and three different selective media. Before processing the samples were homogenized and directly plated to the selective medium. In the filtration method, 10 to 12 drops of a fecal suspension in brain heart infusion were placed on a 0.65-μm-pore-size cellulose acetate filter (Sartorius, Goettingen, Germany) on blood agar base no. 2 (Oxoid) supplemented with 5% sheep blood. After 1 h of incubation at 37°C under aerobic conditions, the filter was removed and the plates were incubated microaerobically. We used as selective agar plates the following media: (i) modified-charcoal cefoperazone deoxycholate agar (mCCDA), a blood-free selective agar base supplemented with 32 μg of cefoperazone/ml and 10 μg of amphotericin/ml (Oxoid CM 739 with Oxoid supplement SR 155) which is widely used in medical, veterinary, and food microbiology laboratories for the isolation of Campylobacter; (ii) cefoperazone amphotericin teicoplanin selective medium (CAT), a blood-free charcoal based agar containing 8 μg of cefoperazone/ml, 4 μg of teicoplanin/ml, and 10 μg of amphotericin/ml (Oxoid supplement SR 174); and (iii) Karmali, a blood-free charcoal-based agar containing 32 μg of cefazolin/ml, 20 μg of vancomycin/ml, and 100 μg of cycloheximide/ml (Oxoid supplement SR 167). The latter two types of plates were used because they are recommended for the detection of Campylobacter upsaliensis and Campylobacter helveticus (2, 10). All plates were incubated at 37°C under microaerobic conditions in jars (Anoxomat, Mart, Lichtenvoorde, The Netherlands) (5% O2, 10% CO2, 85% H2) and examined daily for growth for 4 to 6 days. From plates with growth of Campylobacter suspected (on the basis of colony morphology, catalase, oxidase, and Gram staining results), multiple subcultures of separate colonies (an average of 12 per sample) were grown on blood agar base no. 2 (Oxoid) supplemented with 5% sheep blood. The cultures were identified by PCR-restriction fragment length polymorphism with a method that distinguishes Campylobacter, Arcobacter, and Helicobacter by analysis of the 16S rRNA gene (11). Campylobacter species were identified by PCR-restriction fragment length polymorphism analysis of a highly polymorphic part of the 23S rRNA gene (5) and of the 16S rRNA gene (11). The patterns were compared with those of reference strains obtained from the CCUG/LMG (Culture Collection Universiteit of Ghent, Ghent, Belgium).

Campylobacter species detected in fecal samples from dogs by filter, mCCDA, CAT, and Karmali

Efficiency of isolation.

The use of different kinds of growth media increased the sensitivity of Campylobacter isolation (Table (Table1),1), as described before (4, 14). Overall, 23 (77%) of the samples were found positive for Campylobacter by one or more of the methods. Of the 23 positive-testing dog samples, 16 (70%) harbored C. upsaliensis, 12 (52%) harbored C. jejuni, and 3 (13%) harbored C. lari. This distribution is consistent with other reports on dogs (3, 9, 12, 16).

Incidence of Campylobacter species grown from dog feces as determined by four isolation protocols


By analysis of an average of 12 single colonies from each positive-testing sample, multiple Campylobacter species were observed in 6 (26%) of the 23 positive-testing stool samples (Table (Table2).2). In two samples, as many as three species could be isolated. No systematic study on coinfection with Campylobacter species in dogs has been reported before to our knowledge, although the presence of two Campylobacter species (7, 20) or serotypes (7) in a single sample has been mentioned. However, our results are comparable with those found in investigations of cats, for which it has been demonstrated that 34% of the Campylobacter-positive samples from healthy animals contained more than one Campylobacter species (17).

Healthy versus diarrheic animals.

We examined fecal samples from diarrheic animals (n = 8) as well as from healthy animals (n = 22). Multiple Campylobacter species were detected only in samples from healthy animals (Table (Table2).2). No conclusions can be drawn, however, because of the limited number of samples and since most samples of diarrheic animals had a sampling-to-processing time that exceeded 4 h.


The selective media showed comparable isolation rates, whereas the filtration method was less sensitive (Table (Table1),1), possibly because samples with low numbers of bacteria have been shown to give negative results (8, 18). The distribution of species isolated with the various methods is shown in Table Table2.2. Our results do not confirm that CAT and Karmali media are better suited for detection of C. upsaliensis, notably, as we found a higher number of mCCDA plates positive for C. upsaliensis than resulted using the Karmali method, CAT method, and filtration method, which were specifically introduced for the detection of this Campylobacter species (2, 10). Although the filtration method is recommended for the detection of Campylobacter strains that might be inhibited by antibiotics present in selective media, we did not detect additional Campylobacter species using this method.

Karmali medium performed best in the detection of multiple species from single samples, as two species were recovered from 5 (28%) of the 18 positive-testing samples. The filter method failed to detect any coinfection. As expected, the chance of detecting coinfection was increased by the use of multiple isolation methods.

Transport time.

An influence of the time interval between sampling and processing of the sample in the laboratory was observed, as coinfection was detected only in samples processed within 4 h after collection (Table (Table2).2). Some samples yielded a single species even after 3 days, on the other hand, indicating a considerable variation in the survival times of campylobacters between samples and/or differences in the viability of Campylobacter species.

In conclusion, despite a relatively low number of samples our data clearly show that coinfections of different Campylobacter species are quite common in dogs. The possibility cannot be excluded that the diversity might be even more extensive, as different strains might exist within a single species. This notion is important for epidemiological studies, e.g., the tracing of sources of human infections and studies on the pathogenicity of Campylobacter in dogs.

For studying Campylobacter infections in dogs and cats multiple colonies should be examined, preferably from a combination of media and from specimens that are processed within 4 h after sampling.


The help of the technicians from the Veterinary Microbiological Diagnostic Center of the Veterinary Faculty is gratefully acknowledged. We also thank Alan Rigter for the helpful assistance in the speciation of the strains and Jos van Putten for his critical reading of the manuscript.


1. Allos, B. M. 2001. Campylobacter jejuni infections: update on emerging issues and trends. Clin. Infect. Dis. 32:1201-1206. [PubMed]
2. Aspinall, S. T., D. R. Wareing, P. G. Hayward, and D. N. Hutchinson. 1993. Selective medium for thermophilic campylobacters including Campylobacter upsaliensis. J. Clin. Pathol. 46:829-831. [PMC free article] [PubMed]
3. Baker, J., M. D. Barton, and J. Lanser. 1999. Campylobacter species in cats and dogs in South Australia. Aust. Vet. J. 77:662-666. [PubMed]
4. Bolton, F. J., D. Coates, P. M. Hinchliffe, and L. Robertson. 1983. Comparison of selective media for isolation of Campylobacter jejuni/coli. J. Clin. Pathol. 36:78-83. [PMC free article] [PubMed]
5. Fermér, C., and E. O. Engvall. 1999. Specific PCR identification and differentiation of the thermophilic campylobacters, Campylobacter jejuni, C. coli, C. lari, and C. upsaliensis. J. Clin. Microbiol. 37:3370-3373. [PMC free article] [PubMed]
6. Foley, J. E., S. L. Marks, L. Munson, A. Melli, F. E. Dewhirst, S. Yu, Z. Shen, and J. G. Fox. 1999. Isolation of Helicobacter canis from a colony of bengal cats with endemic diarrhea. J. Clin. Microbiol. 37:3271-3275. [PMC free article] [PubMed]
7. Gondrosen, B., T. Knaevelsrud, and K. Dommarsnes. 1985. Isolation of thermophilic Campylobacters from Norwegian dogs and cats. Acta Vet. Scand. 26:81-90. [PubMed]
8. Goossens, H., L. Vlaes, J. P. Butzler, A. Adnet, P. Hanicq, S. N′Jufom, D. Massart, G. de Schrijver, and W. Blomme. 1991. Campylobacter upsaliensis enteritis associated with canine infections. Lancet 337:1486-1487. [PubMed]
9. Hald, B., and M. Madsen. 1997. Healthy puppies and kittens as carriers of Campylobacter spp., with special reference to Campylobacter upsaliensis. J. Clin. Microbiol. 35:3351-3352. [PMC free article] [PubMed]
10. Karmali, M. A., A. E. Simor, M. Roscoe, P. C. Fleming, S. S. Smith, and J. Lane. 1986. Evaluation of a blood-free, charcoal-based, selective medium for the isolation of Campylobacter organisms from feces. J. Clin. Microbiol. 23:456-459. [PMC free article] [PubMed]
11. Marshall, S. M., P. L. Melito, D. L. Woodward, W. M. Johnson, F. G. Rodgers, and M. R. Mulvey. 1999. Rapid identification of Campylobacter, Arcobacter, and Helicobacter isolates by PCR-restriction fragment length polymorphism analysis of the 16S rRNA gene. J. Clin. Microbiol. 37:4158-4160. [PMC free article] [PubMed]
12. Moser, I., B. Rieksneuwöhner, P. Lentzsch, P. Schwerk, and L. H. Wieler. 2001. Genomic heterogeneity and O-antigenic diversity of Campylobacter upsaliensis and Campylobacter helveticus strains isolated from dogs and cats in Germany. J. Clin. Microbiol. 39:2548-2557. [PMC free article] [PubMed]
13. On, S. L. 1996. Identification methods for campylobacters, helicobacters, and related organisms. Clin. Microbiol. Rev. 9:405-422. [PMC free article] [PubMed]
14. Patton, C. M., S. W. Mitchell, M. E. Potter, and A. F. Kaufmann. 1981. Comparison of selective media for primary isolation of Campylobacter fetus subsp. jejuni. J. Clin. Microbiol. 13:326-330. [PMC free article] [PubMed]
15. Richardson, J. F., J. A. Frost, J. M. Kramer, R. T. Thwaites, F. J. Bolton, D. R. Wareing, and J. A. Gordon. 2001. Coinfection with Campylobacter species: an epidemiological problem? J. Appl. Microbiol. 91:206-211. [PubMed]
16. Sandstedt, K., J. Ursing, and M. Walder. 1983. Thermotolerant Campylobacter with no or weak catalase activity isolated from dogs. Curr. Microbiol. 8:209-213.
17. Shen, Z., Y. Feng, F. E. Dewhirst, and J. G. Fox. 2001. Coinfection of enteric Helicobacter spp. and Campylobacter spp. in cats. J. Clin. Microbiol. 39:2166-2172. [PMC free article] [PubMed]
18. Steele, T. W., and S. N. McDermott. 1984. The use of membrane filters applied directly to the surface of agar plates for the isolation of Campylobacter jejuni from feces. Pathology 16:263-265. [PubMed]
19. Tauxe, R. V., D. A. Pegues, and N. Hargrett-Bean. 1987. Campylobacter infections: the emerging national pattern. Am. J. Public Health 77:1219-1221. [PMC free article] [PubMed]
20. Treschnak, E., and E. Hellmann. 1987. Comparison of different Campylobacter-selective media in the study of feces samples of domestic animals. Berl. Muench. Tieraerztl. Wochenschr. 100:381-385. [PubMed]

Articles from Journal of Clinical Microbiology are provided here courtesy of American Society for Microbiology (ASM)
PubReader format: click here to try


Related citations in PubMed

See reviews...See all...

Cited by other articles in PMC

See all...