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Figure 4

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Brain sections reveal extravascular trypanosomes after low-dose infection.

Screening of large areas of brain tissue was required to detect extravascular (A, arrow) and intravascular (B, arrow) Tbb-O (red) 24 h after infection with 105 BSF. Infected mice were injected with BSA-Alexa 647 (green) and the nuclear stain Hoechst (blue) prior to removal of the brain. Immediately after vibratome sectioning, 100 µm slices of live brain tissue were subjected to ex vivo imaging. Traces of the vascular marker remain visible after sectioning and outline the microvascular lumen. Bars = 20 µm.

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