• We are sorry, but NCBI web applications do not support your browser and may not function properly. More information
Logo of narLink to Publisher's site
Nucleic Acids Res. May 11, 1987; 15(9): 3773–3786.
PMCID: PMC340781

Oestrogen regulates cathepsin D mRNA levels in oestrogen responsive human breast cancer cells.


A cDNA library was constructed from the mRNA of the ZR-75 oestrogen responsive human breast cancer cell line and screened for oestrogen regulated mRNA sequences. Of the recombinants isolated, 30 contained cDNA that corresponded to a single mRNA species of 2.1 kb that was induced between 10 and 15 fold by oestradiol treatment. The sequence of the entire open reading frame and 3' non-coding region of the mRNA was determined and shown to encode the aspartyl protease cathepsin D. The induction of cathepsin D mRNA is specific for oestrogen and is maximal at 3 X 10(-10) M. Cathepsin D mRNA levels were increased by oestrogen in 3 oestrogen responsive breast cancer cell lines. Cathepsin D mRNA was expressed but not regulated in an oestrogen receptor negative breast tumour cell line, BT 20, and in 2 other malignant cell lines, Hela and A431.

Full text

Full text is available as a scanned copy of the original print version. Get a printable copy (PDF file) of the complete article (1.5M), or click on a page image below to browse page by page. Links to PubMed are also available for Selected References.

Images in this article

Selected References

These references are in PubMed. This may not be the complete list of references from this article.
  • Osborne CK, Yochmowitz MG, Knight WA, 3rd, McGuire WL. The value of estrogen and progesterone receptors in the treatment of breast cancer. Cancer. 1980 Dec 15;46(12 Suppl):2884–2888. [PubMed]
  • Lippman ME, Bolan G. Oestrogen-responsive human breast cancer in long term tissue culture. Nature. 1975 Aug 14;256(5518):592–593. [PubMed]
  • Masiakowski P, Breathnach R, Bloch J, Gannon F, Krust A, Chambon P. Cloning of cDNA sequences of hormone-regulated genes from the MCF-7 human breast cancer cell line. Nucleic Acids Res. 1982 Dec 20;10(24):7895–7903. [PMC free article] [PubMed]
  • May FE, Westley BR. Cloning of estrogen-regulated messenger RNA sequences from human breast cancer cells. Cancer Res. 1986 Dec;46(12 Pt 1):6034–6040. [PubMed]
  • Westley B, Rochefort H. A secreted glycoprotein induced by estrogen in human breast cancer cell lines. Cell. 1980 Jun;20(2):353–362. [PubMed]
  • Edwards DP, Adams DJ, Savage N, McGuire WL. Estrogen induced synthesis of specific proteins in human breast cancer cells. Biochem Biophys Res Commun. 1980 Apr 14;93(3):804–812. [PubMed]
  • Horwitz KB, Koseki Y, McGuire WL. Estrogen control of progesterone receptor in human breast cancer: role of estradiol and antiestrogen. Endocrinology. 1978 Nov;103(5):1742–1751. [PubMed]
  • Dickson RB, Bates SE, McManaway ME, Lippman ME. Characterization of estrogen responsive transforming activity in human breast cancer cell lines. Cancer Res. 1986 Apr;46(4 Pt 1):1707–1713. [PubMed]
  • Soule HD, Vazguez J, Long A, Albert S, Brennan M. A human cell line from a pleural effusion derived from a breast carcinoma. J Natl Cancer Inst. 1973 Nov;51(5):1409–1416. [PubMed]
  • Engel LW, Young NA, Tralka TS, Lippman ME, O'Brien SJ, Joyce MJ. Establishment and characterization of three new continuous cell lines derived from human breast carcinomas. Cancer Res. 1978 Oct;38(10):3352–3364. [PubMed]
  • Keydar I, Chen L, Karby S, Weiss FR, Delarea J, Radu M, Chaitcik S, Brenner HJ. Establishment and characterization of a cell line of human breast carcinoma origin. Eur J Cancer. 1979 May;15(5):659–670. [PubMed]
  • LASFARGUES EY, OZZELLO L. Cultivation of human breast carcinomas. J Natl Cancer Inst. 1958 Dec;21(6):1131–1147. [PubMed]
  • Fabricant RN, De Larco JE, Todaro GJ. Nerve growth factor receptors on human melanoma cells in culture. Proc Natl Acad Sci U S A. 1977 Feb;74(2):565–569. [PMC free article] [PubMed]
  • Goldberg DA. Isolation and partial characterization of the Drosophila alcohol dehydrogenase gene. Proc Natl Acad Sci U S A. 1980 Oct;77(10):5794–5798. [PMC free article] [PubMed]
  • Southern EM. Detection of specific sequences among DNA fragments separated by gel electrophoresis. J Mol Biol. 1975 Nov 5;98(3):503–517. [PubMed]
  • Rigby PW, Dieckmann M, Rhodes C, Berg P. Labeling deoxyribonucleic acid to high specific activity in vitro by nick translation with DNA polymerase I. J Mol Biol. 1977 Jun 15;113(1):237–251. [PubMed]
  • Melton DA, Krieg PA, Rebagliati MR, Maniatis T, Zinn K, Green MR. Efficient in vitro synthesis of biologically active RNA and RNA hybridization probes from plasmids containing a bacteriophage SP6 promoter. Nucleic Acids Res. 1984 Sep 25;12(18):7035–7056. [PMC free article] [PubMed]
  • Westley B, May FE. The human genome contains multiple sequences of varying homology to mouse mammary tumour virus DNA. Gene. 1984 May;28(2):221–227. [PubMed]
  • Queen C, Korn LJ. A comprehensive sequence analysis program for the IBM personal computer. Nucleic Acids Res. 1984 Jan 11;12(1 Pt 2):581–599. [PMC free article] [PubMed]
  • Shewale JG, Tang J. Amino acid sequence of porcine spleen cathepsin D. Proc Natl Acad Sci U S A. 1984 Jun;81(12):3703–3707. [PMC free article] [PubMed]
  • Faust PL, Kornfeld S, Chirgwin JM. Cloning and sequence analysis of cDNA for human cathepsin D. Proc Natl Acad Sci U S A. 1985 Aug;82(15):4910–4914. [PMC free article] [PubMed]
  • Chalbos D, Vignon F, Keydar I, Rochefort H. Estrogens stimulate cell proliferation and induce secretory proteins in a human breast cancer cell line (T47D). J Clin Endocrinol Metab. 1982 Aug;55(2):276–283. [PubMed]
  • Tang J. Evolution in the structure and function of carboxyl proteases. Mol Cell Biochem. 1979 Jul 31;26(2):93–109. [PubMed]
  • Elangovan S, Moulton BC. Progesterone and estrogen control of rates of synthesis of uterine cathepsin D. J Biol Chem. 1980 Aug 10;255(15):7474–7479. [PubMed]
  • Butler WB, Kirkland WL, Jorgensen TL. Induction of plasminogen activator by estrogen in a human breast cancer cell line (MCF-7). Biochem Biophys Res Commun. 1979 Oct 29;90(4):1328–1334. [PubMed]
  • Jakowlew SB, Breathnach R, Jeltsch JM, Masiakowski P, Chambon P. Sequence of the pS2 mRNA induced by estrogen in the human breast cancer cell line MCF-7. Nucleic Acids Res. 1984 Mar 26;12(6):2861–2878. [PMC free article] [PubMed]
  • Morisset M, Capony F, Rochefort H. The 52-kDa estrogen-induced protein secreted by MCF7 cells is a lysosomal acidic protease. Biochem Biophys Res Commun. 1986 Jul 16;138(1):102–109. [PubMed]
  • Westley B, May FE, Brown AM, Krust A, Chambon P, Lippman ME, Rochefort H. Effects of antiestrogens on the estrogen-regulated pS2 RNA and the 52- and 160-kilodalton proteins in MCF7 cells and two tamoxifen-resistant sublines. J Biol Chem. 1984 Aug 25;259(16):10030–10035. [PubMed]
  • Recklies AD, Tiltman KJ, Stoker TA, Poole AR. Secretion of proteinases from malignant and nonmalignant human breast tissue. Cancer Res. 1980 Mar;40(3):550–556. [PubMed]
  • Capony F, Garcia M, Capdevielle J, Rougeot C, Ferrara P, Rochefort H. Purification and first characterization of the secreted and cellular 52-kDa proteins regulated by estrogens in human-breast cancer cells. Eur J Biochem. 1986 Dec 1;161(2):505–512. [PubMed]
  • Garcia M, Salazar-Retana G, Pages A, Richer G, Domergue J, Pages AM, Cavalie G, Martin JM, Lamarque JL, Pau B, et al. Distribution of the Mr 52,000 estrogen-regulated protein in benign breast diseases and other tissues by immunohistochemistry. Cancer Res. 1986 Jul;46(7):3734–3738. [PubMed]
  • Sloane BF, Honn KV, Sadler JG, Turner WA, Kimpson JJ, Taylor JD. Cathepsin B activity in B16 melanoma cells: a possible marker for metastatic potential. Cancer Res. 1982 Mar;42(3):980–986. [PubMed]
  • Gal S, Gottesman MM. The major excreted protein of transformed fibroblasts is an activable acid-protease. J Biol Chem. 1986 Feb 5;261(4):1760–1765. [PubMed]
  • Morrison RI, Barrett AJ, Dingle JT, Prior D. Cathepsins BI and D. Action on human cartilage proteoglycans. Biochim Biophys Acta. 1973 Apr 12;302(2):411–419. [PubMed]
  • Terayama H, Morioka M, Koji T. Mitogenic effects of certain cathepsins and calciferin on the intact liver in vivo. Int J Biochem. 1985;17(9):949–955. [PubMed]

Articles from Nucleic Acids Research are provided here courtesy of Oxford University Press


Related citations in PubMed

See reviews...See all...

Cited by other articles in PMC

See all...


Recent Activity

Your browsing activity is empty.

Activity recording is turned off.

Turn recording back on

See more...