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Nucleic Acids Res. Mar 11, 1987; 15(5): 2327–2341.
PMCID: PMC340637

A new moderately repetitive DNA sequence family of novel organization.

Abstract

In cloning adenovirus homologous sequences, from a human cosmid library, we identified a moderately repetitive DNA sequence family consisting of tandem arrays of 2.5 kb members. A member was sequenced and several non-adjacent, 15-20 bp G-C rich segments with homology to the left side of adenovirus were discovered. The copy number of 400 members is highly conserved among humans. Southern blots of partial digests of human DNA have verified the tandem array of the sequence family. The chromosomal location was defined by somatic cell genetics and in situ hybridization. Tandem arrays are found only on chromosomes 4 (4q31) and 19 (q13.1-q13.5). Homologous repetitive sequences are found in DNA of other primates but not in cat or mouse. Thus we have identified a new family of moderately repetitive DNA sequences, unique because of its organization in clustered tandem arrays, its length, its chromosomal location, and its lack of homology to other moderately repetitive sequence families.

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Selected References

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  • Grodzicker T, Klessig DF. Expression of unselected adenovirus genes in human cells co-transformed with the HSV-1 tk gene and adenovirus 2 DNA. Cell. 1980 Sep;21(2):453–463. [PubMed]
  • Braithwaite AW, Lejeune S, Naora H. Adenoviruses have homology with a reiterated sequence in genomic DNA. DNA. 1984 Jun;3(3):223–230. [PubMed]
  • Karlsson S, Humphries RK, Gluzman Y, Nienhuis AW. Transfer of genes into hematopoietic cells using recombinant DNA viruses. Proc Natl Acad Sci U S A. 1985 Jan;82(1):158–162. [PMC free article] [PubMed]
  • Singer MF. SINEs and LINEs: highly repeated short and long interspersed sequences in mammalian genomes. Cell. 1982 Mar;28(3):433–434. [PubMed]
  • Sun L, Paulson KE, Schmid CW, Kadyk L, Leinwand L. Non-Alu family interspersed repeats in human DNA and their transcriptional activity. Nucleic Acids Res. 1984 Mar 26;12(6):2669–2690. [PMC free article] [PubMed]
  • Adams JW, Kaufman RE, Kretschmer PJ, Harrison M, Nienhuis AW. A family of long reiterated DNA sequences, one copy of which is next to the human beta globin gene. Nucleic Acids Res. 1980 Dec 20;8(24):6113–6128. [PMC free article] [PubMed]
  • Hattori M, Hidaka S, Sakaki Y. Sequence analysis of a KpnI family member near the 3' end of human beta-globin gene. Nucleic Acids Res. 1985 Nov 11;13(21):7813–7827. [PMC free article] [PubMed]
  • Shafit-Zagardo B, Brown FL, Zavodny PJ, Maio JJ. Transcription of the KpnI families of long interspersed DNAs in human cells. Nature. 1983 Jul 21;304(5923):277–280. [PubMed]
  • Tashima M, Calabretta B, Torelli G, Scofield M, Maizel A, Saunders GF. Presence of a highly repetitive and widely dispersed DNA sequence in the human genome. Proc Natl Acad Sci U S A. 1981 Mar;78(3):1508–1512. [PMC free article] [PubMed]
  • Jagadeeswaran P, Forget BG, Weissman SM. Short interspersed repetitive DNA elements in eucaryotes: transposable DNA elements generated by reverse transcription of RNA pol III transcripts? Cell. 1981 Oct;26(2 Pt 2):141–142. [PubMed]
  • Elder JT, Pan J, Duncan CH, Weissman SM. Transcriptional analysis of interspersed repetitive polymerase III transcription units in human DNA. Nucleic Acids Res. 1981 Mar 11;9(5):1171–1189. [PMC free article] [PubMed]
  • Jelinek WR, Toomey TP, Leinwand L, Duncan CH, Biro PA, Choudary PV, Weissman SM, Rubin CM, Houck CM, Deininger PL, et al. Ubiquitous, interspersed repeated sequences in mammalian genomes. Proc Natl Acad Sci U S A. 1980 Mar;77(3):1398–1402. [PMC free article] [PubMed]
  • Ullu E, Murphy S, Melli M. Human 7SL RNA consists of a 140 nucleotide middle-repetitive sequence inserted in an alu sequence. Cell. 1982 May;29(1):195–202. [PubMed]
  • Henderson AS, Warburton D, Atwood KC. Location of ribosomal DNA in the human chromosome complement. Proc Natl Acad Sci U S A. 1972 Nov;69(11):3394–3398. [PMC free article] [PubMed]
  • Wellauer PK, Dawid IB. Isolation and sequence organization of human ribosomal DNA. J Mol Biol. 1979 Mar 5;128(3):289–303. [PubMed]
  • Erickson JM, Schmickel RD. A molecular basis for discrete size variation in human ribosomal DNA. Am J Hum Genet. 1985 Mar;37(2):311–325. [PMC free article] [PubMed]
  • Lindgren V, Ares M, Jr, Weiner AM, Francke U. Human genes for U2 small nuclear RNA map to a major adenovirus 12 modification site on chromosome 17. Nature. 1985 Mar 7;314(6006):115–116. [PubMed]
  • Van Arsdell SW, Weiner AM. Human genes for U2 small nuclear RNA are tandemly repeated. Mol Cell Biol. 1984 Mar;4(3):492–499. [PMC free article] [PubMed]
  • Green L, Van Antwerpen R, Stein J, Stein G, Tripputi P, Emanuel B, Selden J, Croce C. A major human histone gene cluster on the long arm of chromosome 1. Science. 1984 Nov 16;226(4676):838–840. [PubMed]
  • Marashi F, Prokopp K, Stein J, Stein G. Evidence for a human histone gene cluster containing H2B and H2A pseudogenes. Proc Natl Acad Sci U S A. 1984 Apr;81(7):1936–1940. [PMC free article] [PubMed]
  • Sierra F, Lichtler A, Marashi F, Rickles R, Van Dyke T, Clark S, Wells J, Stein G, Stein J. Organization of human histone genes. Proc Natl Acad Sci U S A. 1982 Mar;79(6):1795–1799. [PMC free article] [PubMed]
  • Southern EM. Detection of specific sequences among DNA fragments separated by gel electrophoresis. J Mol Biol. 1975 Nov 5;98(3):503–517. [PubMed]
  • Chen MJ, Shimada T, Moulton AD, Cline A, Humphries RK, Maizel J, Nienhuis AW. The functional human dihydrofolate reductase gene. J Biol Chem. 1984 Mar 25;259(6):3933–3943. [PubMed]
  • Grosveld FG, Dahl HH, de Boer E, Flavell RA. Isolation of beta-globin-related genes from a human cosmid library. Gene. 1981 Apr;13(3):227–237. [PubMed]
  • Poncz M, Solowiejczyk D, Ballantine M, Schwartz E, Surrey S. "Nonrandom" DNA sequence analysis in bacteriophage M13 by the dideoxy chain-termination method. Proc Natl Acad Sci U S A. 1982 Jul;79(14):4298–4302. [PMC free article] [PubMed]
  • Sanger F, Nicklen S, Coulson AR. DNA sequencing with chain-terminating inhibitors. Proc Natl Acad Sci U S A. 1977 Dec;74(12):5463–5467. [PMC free article] [PubMed]
  • Harper ME, Saunders GF. Localization of single copy DNA sequences of G-banded human chromosomes by in situ hybridization. Chromosoma. 1981;83(3):431–439. [PubMed]
  • Queen C, Korn LJ. A comprehensive sequence analysis program for the IBM personal computer. Nucleic Acids Res. 1984 Jan 11;12(1 Pt 2):581–599. [PMC free article] [PubMed]
  • Bell GI, Selby MJ, Rutter WJ. The highly polymorphic region near the human insulin gene is composed of simple tandemly repeating sequences. Nature. 1982 Jan 7;295(5844):31–35. [PubMed]
  • Spritz RA. Duplication/deletion polymorphism 5' - to the human beta globin gene. Nucleic Acids Res. 1981 Oct 10;9(19):5037–5047. [PMC free article] [PubMed]
  • Schmid CW, Jelinek WR. The Alu family of dispersed repetitive sequences. Science. 1982 Jun 4;216(4550):1065–1070. [PubMed]
  • Daniels GR, Deininger PL. Repeat sequence families derived from mammalian tRNA genes. Nature. 317(6040):819–822. [PubMed]
  • Paulson KE, Deka N, Schmid CW, Misra R, Schindler CW, Rush MG, Kadyk L, Leinwand L. A transposon-like element in human DNA. Nature. 1985 Jul 25;316(6026):359–361. [PubMed]
  • Busch H, Reddy R, Rothblum L, Choi YC. SnRNAs, SnRNPs, and RNA processing. Annu Rev Biochem. 1982;51:617–654. [PubMed]
  • Naylor SL, Zabel BU, Manser T, Gesteland R, Sakaguchi AY. Localization of human U1 small nuclear RNA genes to band p36.3 of chromosome 1 by in situ hybridization. Somat Cell Mol Genet. 1984 May;10(3):307–313. [PubMed]
  • Lund E, Bostock C, Robertson M, Christie S, Mitchen JL, Dahlberg JE. U1 small nuclear RNA genes are located on human chromosome 1 and are expressed in mouse-human hybrid cells. Mol Cell Biol. 1983 Dec;3(12):2211–2220. [PMC free article] [PubMed]
  • Denison RA, Van Arsdell SW, Bernstein LB, Weiner AM. Abundant pseudogenes for small nuclear RNAs are dispersed in the human genome. Proc Natl Acad Sci U S A. 1981 Feb;78(2):810–814. [PMC free article] [PubMed]
  • Ohshima Y, Itoh M, Okada N, Miyata T. Novel models for RNA splicing that involve a small nuclear RNA. Proc Natl Acad Sci U S A. 1981 Jul;78(7):4471–4474. [PMC free article] [PubMed]
  • Branlant C, Krol A, Ebel JP, Lazar E, Haendler B, Jacob M. U2 RNA shares a structural domain with U1, U4, and U5 RNAs. EMBO J. 1982;1(10):1259–1265. [PMC free article] [PubMed]
  • Tripputi P, Emanuel BS, Croce CM, Green LG, Stein GS, Stein JL. Human histone genes map to multiple chromosomes. Proc Natl Acad Sci U S A. 1986 May;83(10):3185–3188. [PMC free article] [PubMed]
  • Orgel LE, Crick FH, Sapienza C. Selfish DNA. Nature. 1980 Dec 25;288(5792):645–646. [PubMed]
  • Orgel LE, Crick FH. Selfish DNA: the ultimate parasite. Nature. 1980 Apr 17;284(5757):604–607. [PubMed]
  • Doolittle WF, Sapienza C. Selfish genes, the phenotype paradigm and genome evolution. Nature. 1980 Apr 17;284(5757):601–603. [PubMed]
  • Roberts JM, Axel R. Gene amplification and gene correction in somatic cells. Cell. 1982 May;29(1):109–119. [PubMed]
  • Slightom JL, Blechl AE, Smithies O. Human fetal G gamma- and A gamma-globin genes: complete nucleotide sequences suggest that DNA can be exchanged between these duplicated genes. Cell. 1980 Oct;21(3):627–638. [PubMed]
  • Liskay RM, Stachelek JL. Evidence for intrachromosomal gene conversion in cultured mouse cells. Cell. 1983 Nov;35(1):157–165. [PubMed]
  • Weiss EH, Mellor A, Golden L, Fahrner K, Simpson E, Hurst J, Flavell RA. The structure of a mutant H-2 gene suggests that the generation of polymorphism in H-2 genes may occur by gene conversion-like events. Nature. 1983 Feb 24;301(5902):671–674. [PubMed]
  • Smith GP. Evolution of repeated DNA sequences by unequal crossover. Science. 1976 Feb 13;191(4227):528–535. [PubMed]
  • Yunis JJ, Soreng AL. Constitutive fragile sites and cancer. Science. 1984 Dec 7;226(4679):1199–1204. [PubMed]
  • Schnitzer TJ, Weiss RA, Juricek DK, Ruddle FH. Use of vesicular stomatitis virus pseudotypes to map viral receptor genes: Assignment of RD114 virus receptor gene to human chromosome 19. J Virol. 1980 Aug;35(2):575–580. [PMC free article] [PubMed]

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