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Nucleic Acids Res. 1987 Feb 11; 15(3): 959–970.
PMCID: PMC340501

Genetic organization of the c-sis transcription unit.


The structure and genetic organization of the transcription unit of the c-sis proto-oncogene was determined. Comparative nucleotide sequence analysis of the exon sequences of feline and human c-sis revealed a very high degree of homology. The cap site as well as the poly(A)-addition site of the sis transcript of each species was identified and found in similar positions. An insert of 4 amino acids was found in the deduced translational product of feline c-sis and it was located at the amino-terminus of the region that constituted the platelet-derived growth factor domain. An insert of 149 bp present at the 5' end of exon 7 of human c-sis but absent in the simian sarcoma virus v-sis oncogene was also present in the feline c-sis proto-oncogene.

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Selected References

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  • Doolittle RF, Hunkapiller MW, Hood LE, Devare SG, Robbins KC, Aaronson SA, Antoniades HN. Simian sarcoma virus onc gene, v-sis, is derived from the gene (or genes) encoding a platelet-derived growth factor. Science. 1983 Jul 15;221(4607):275–277. [PubMed]
  • Waterfield MD, Scrace GT, Whittle N, Stroobant P, Johnsson A, Wasteson A, Westermark B, Heldin CH, Huang JS, Deuel TF. Platelet-derived growth factor is structurally related to the putative transforming protein p28sis of simian sarcoma virus. Nature. 1983 Jul 7;304(5921):35–39. [PubMed]
  • Robbins KC, Antoniades HN, Devare SG, Hunkapiller MW, Aaronson SA. Structural and immunological similarities between simian sarcoma virus gene product(s) and human platelet-derived growth factor. Nature. 1983 Oct 13;305(5935):605–608. [PubMed]
  • Irgens K, Wyers M, Moraillon A, Parodi A, Fortuny V. Isolement d'un virus sarcomatogène félin à partir d'un fibrosarcome spontané du chat: étude du pouvoir sarcomatogène in vivo. C R Acad Sci Hebd Seances Acad Sci D. 1973 Mar 12;276(11):1783–1786. [PubMed]
  • Besmer P, Snyder HW, Jr, Murphy JE, Hardy WD, Jr, Parodi A. The Parodi-Irgens feline sarcoma virus and simian sarcoma virus have homologous oncogenes, but in different contexts of the viral genomes. J Virol. 1983 May;46(2):606–613. [PMC free article] [PubMed]
  • Hannink M, Donoghue DJ. Requirement for a signal sequence in biological expression of the v-sis oncogene. Science. 1984 Dec 7;226(4679):1197–1199. [PubMed]
  • King CR, Giese NA, Robbins KC, Aaronson SA. In vitro mutagenesis of the v-sis transforming gene defines functional domains of its growth factor-related product. Proc Natl Acad Sci U S A. 1985 Aug;82(16):5295–5299. [PMC free article] [PubMed]
  • Kelly JD, Raines EW, Ross R, Murray MJ. The B chain of PDGF alone is sufficient for mitogenesis. EMBO J. 1985 Dec 16;4(13A):3399–3405. [PMC free article] [PubMed]
  • Chiu IM, Reddy EP, Givol D, Robbins KC, Tronick SR, Aaronson SA. Nucleotide sequence analysis identifies the human c-sis proto-oncogene as a structural gene for platelet-derived growth factor. Cell. 1984 May;37(1):123–129. [PubMed]
  • Josephs SF, Guo C, Ratner L, Wong-Staal F. Human-proto-oncogene nucleotide sequences corresponding to the transforming region of simian sarcoma virus. Science. 1984 Feb 3;223(4635):487–491. [PubMed]
  • Johnsson A, Heldin CH, Wasteson A, Westermark B, Deuel TF, Huang JS, Seeburg PH, Gray A, Ullrich A, Scrace G, et al. The c-sis gene encodes a precursor of the B chain of platelet-derived growth factor. EMBO J. 1984 May;3(5):921–928. [PMC free article] [PubMed]
  • Van den Ouweland AM, Roebroek AJ, Schalken JA, Claesen CA, Bloemers HP, Van de Ven WJ. Structure and nucleotide sequence of the 5' region of the human and feline c-sis proto-oncogenes. Nucleic Acids Res. 1986 Jan 24;14(2):765–778. [PMC free article] [PubMed]
  • Collins T, Ginsburg D, Boss JM, Orkin SH, Pober JS. Cultured human endothelial cells express platelet-derived growth factor B chain: cDNA cloning and structural analysis. Nature. 1985 Aug 22;316(6030):748–750. [PubMed]
  • Ratner L, Josephs SF, Jarrett R, Reitz MS, Jr, Wong-Staal F. Nucleotide sequence of transforming human c-sis cDNA clones with homology to platelet-derived growth factor. Nucleic Acids Res. 1985 Jul 25;13(14):5007–5018. [PMC free article] [PubMed]
  • Rao CD, Igarashi H, Chiu IM, Robbins KC, Aaronson SA. Structure and sequence of the human c-sis/platelet-derived growth factor 2 (SIS/PDGF2) transcriptional unit. Proc Natl Acad Sci U S A. 1986 Apr;83(8):2392–2396. [PMC free article] [PubMed]
  • Takeda K, Minowada J, Bloch A. Kinetics of appearance of differentiation-associated characteristics in ML-1, a line of human myeloblastic leukemia cells, after treatment with 12-O-tetradecanoylphorbol-13-acetate, dimethyl sulfoxide or 1-beta-D-arabinofuranosylcytosine. Cancer Res. 1982 Dec;42(12):5152–5158. [PubMed]
  • Dodemont HJ, Soriano P, Quax WJ, Ramaekers F, Lenstra JA, Groenen MA, Bernardi G, Bloemendal H. The genes coding for the cytoskeletal proteins actin and vimentin in warm-blooded vertebrates. EMBO J. 1982;1(2):167–171. [PMC free article] [PubMed]
  • Messing J, Vieira J. A new pair of M13 vectors for selecting either DNA strand of double-digest restriction fragments. Gene. 1982 Oct;19(3):269–276. [PubMed]
  • Sanger F, Nicklen S, Coulson AR. DNA sequencing with chain-terminating inhibitors. Proc Natl Acad Sci U S A. 1977 Dec;74(12):5463–5467. [PMC free article] [PubMed]
  • Staden R. An interactive graphics program for comparing and aligning nucleic acid and amino acid sequences. Nucleic Acids Res. 1982 May 11;10(9):2951–2961. [PMC free article] [PubMed]
  • Auffray C, Rougeon F. Purification of mouse immunoglobulin heavy-chain messenger RNAs from total myeloma tumor RNA. Eur J Biochem. 1980 Jun;107(2):303–314. [PubMed]
  • McMaster GK, Carmichael GG. Analysis of single- and double-stranded nucleic acids on polyacrylamide and agarose gels by using glyoxal and acridine orange. Proc Natl Acad Sci U S A. 1977 Nov;74(11):4835–4838. [PMC free article] [PubMed]
  • Schalken JA, van den Ouweland AM, Bloemers HP, van de Ven WJ. Characterization of the feline c-abl proto-oncogene. Biochim Biophys Acta. 1985 Feb 20;824(2):104–112. [PubMed]
  • Roebroek AJ, Schalken JA, Bussemakers MJ, van Heerikhuizen H, Onnekink C, Debruyne FM, Bloemers HP, Van de Ven WJ. Characterization of human c-fes/fps reveals a new transcription unit (fur) in the immediately upstream region of the proto-oncogene. Mol Biol Rep. 1986;11(2):117–125. [PubMed]
  • Hanahan D, Meselson M. Plasmid screening at high colony density. Gene. 1980 Jun;10(1):63–67. [PubMed]
  • van Leen RW, Kastrop PM, van Roozendaal KE, Schoenmakers JG. Sequence divergence and selection of cap sites in the rat gamma-crystallin gene family. Eur J Biochem. 1986 May 15;157(1):203–208. [PubMed]
  • Goustin AS, Betsholtz C, Pfeifer-Ohlsson S, Persson H, Rydnert J, Bywater M, Holmgren G, Heldin CH, Westermark B, Ohlsson R. Coexpression of the sis and myc proto-oncogenes in developing human placenta suggests autocrine control of trophoblast growth. Cell. 1985 May;41(1):301–312. [PubMed]
  • Yarden Y, Escobedo JA, Kuang WJ, Yang-Feng TL, Daniel TO, Tremble PM, Chen EY, Ando ME, Harkins RN, Francke U, et al. Structure of the receptor for platelet-derived growth factor helps define a family of closely related growth factor receptors. Nature. 1986 Sep 18;323(6085):226–232. [PubMed]
  • Shaw G, Kamen R. A conserved AU sequence from the 3' untranslated region of GM-CSF mRNA mediates selective mRNA degradation. Cell. 1986 Aug 29;46(5):659–667. [PubMed]
  • Vogeli G, Ohkubo H, Sobel ME, Yamada Y, Pastan I, de Crombrugghe B. Structure of the promoter for chicken alpha 2 type I collagen gene. Proc Natl Acad Sci U S A. 1981 Sep;78(9):5334–5338. [PMC free article] [PubMed]
  • Ishii S, Xu YH, Stratton RH, Roe BA, Merlino GT, Pastan I. Characterization and sequence of the promoter region of the human epidermal growth factor receptor gene. Proc Natl Acad Sci U S A. 1985 Aug;82(15):4920–4924. [PMC free article] [PubMed]
  • Breathnach R, Chambon P. Organization and expression of eucaryotic split genes coding for proteins. Annu Rev Biochem. 1981;50:349–383. [PubMed]
  • Devare SG, Reddy EP, Law JD, Robbins KC, Aaronson SA. Nucleotide sequence of the simian sarcoma virus genome: demonstration that its acquired cellular sequences encode the transforming gene product p28sis. Proc Natl Acad Sci U S A. 1983 Feb;80(3):731–735. [PMC free article] [PubMed]
  • Betsholtz C, Johnsson A, Heldin CH, Westermark B, Lind P, Urdea MS, Eddy R, Shows TB, Philpott K, Mellor AL, et al. cDNA sequence and chromosomal localization of human platelet-derived growth factor A-chain and its expression in tumour cell lines. Nature. 1986 Apr 24;320(6064):695–699. [PubMed]

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