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Journal List > Reprod Biol Endocrinol > v.2; 2004

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Reprod Biol Endocrinol. 2004; 2: 6.
Published online 2004 January 28. doi: 10.1186/1477-7827-2-6.
PMCID: PMC340388
Copyright © 2004 Michael et al; licensee BioMed Central Ltd. This is an Open Access article: verbatim copying and redistribution of this article are permitted in all media for any purpose, provided this notice is preserved along with the article's original URL.
Induced ovulation and egg deposition in the direct developing anuran Eleutherodactylus coqui
Scott F Michael,1 Christine Buckley,corresponding author2 Esteban Toro,3 Alberto R Estrada,4 and Shawn Vincent2
1Department of Tropical Medicine, Tulane University, New Orleans, Louisiana, USA
2Department of Ecology and Evolutionary Biology, Tulane University, New Orleans, Louisiana, USA
3Departamento de Ciencias Biológicas, Universidad de los Andes, Bogotá, Colombia
4Department of Science, Technology and Health, Universidad Metropolitana, San Juan, Puerto Rico
corresponding authorCorresponding author.
Scott F Michael: smichael/at/tulane.edu; Christine Buckley: cbuckley/at/tulane.edu; Esteban Toro: estetoro/at/hotmail.com; Alberto R Estrada: albertore/at/prtc.net; Shawn Vincent: svincent/at/tulane.edu
Received December 17, 2003; Accepted January 28, 2004.
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Abstract
This study investigates ovulation and egg deposition behaviors in the anuran Eleutherodactylus coqui from Puerto Rico in response to stimulation with gonadotropin and gonadotropin releasing hormones. Five hormones were tested by injection over a range of doses, including mammalian LHRH, avian LHRH, fish LHRH, D-Ala6, des-Gly10 ethylamide LHRH and hCG. We report a low level of ovulation and egg deposition in response to all hormones, with the most complete and consistent results from the non-natural D-Ala6, des-Gly10 ethylamide LHRH derivative. To confirm the viability of eggs produced in this manner we performed in vitro fertilization experiments that resulted in the development of normal frogs. Reproductive behaviors in E. coqui are apparently not controlled by a mammalian form of LHRH as reported in other common laboratory anuran species. D-Ala6, des-Gly10 ethylamide LHRH induces ovulation and deposition of mature and fertilizable eggs in E. coqui.
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