• We are sorry, but NCBI web applications do not support your browser and may not function properly. More information
Logo of narLink to Publisher's site
Nucleic Acids Res. Nov 11, 1988; 16(21): 10323–10338.
PMCID: PMC338854

Interactions of water-soluble metalloporphyrins with nucleic acids studied by resonance Raman spectroscopy.

Abstract

The resonance Raman spectra of water-soluble porphyrins, M(TMpy-P4) (M = Cu(II), Ni(II) and Co(III] and their mixtures with poly(dG-dC)2, poly(dA-dT)2 and calf thymus and salmon DNAs were measured using a divided rotating cell to determine the magnitudes of frequency shift and intensity variation resulting from M(TMpy-P4)-nucleic acid interactions. Bands II(C beta-H bending, approximately 1100 cm-1) and VIII(C beta-C beta stretch, approximately 1570 cm-1) show a large and small upward shift, respectively, when Cu(TMpy-P4) and Ni(TMpy-P4) are intercalated at the G-C sites. In contrast, these bands show a small upward and downward shift, respectively, when Co(TMpy-P4) is groove-bound at the A-T sites of nucleic acids. Both Bands V (approximately 1260 cm-1) and IX (approximately 1646 cm-1) which originate in the N-methylpyridyl group always show small downward shifts due to coulombic interaction between the N-CH3+ group of TMpy-P4 and the PO2 group of the nucleic acid.

Full text

Full text is available as a scanned copy of the original print version. Get a printable copy (PDF file) of the complete article (1.0M), or click on a page image below to browse page by page. Links to PubMed are also available for Selected References.

Selected References

These references are in PubMed. This may not be the complete list of references from this article.
  • Waring MJ. DNA modification and cancer. Annu Rev Biochem. 1981;50:159–192. [PubMed]
  • Jeffrey AM, Weinstein IB, Jennette KW, Grzeskowiak K, Nakanishi K, Harvey RG, Autrup H, Harris C. Structures of benzo(a)pyrene--nucleic acid adducts formed in human and bovine bronchial explants. Nature. 1977 Sep 22;269(5626):348–350. [PubMed]
  • Fiel RJ, Howard JC, Mark EH, Datta Gupta N. Interaction of DNA with a porphyrin ligand: evidence for intercalation. Nucleic Acids Res. 1979 Jul 11;6(9):3093–3118. [PMC free article] [PubMed]
  • Fiel RJ, Munson BR. Binding of meso-tetra (4-N-methylpyridyl) porphine to DNA. Nucleic Acids Res. 1980 Jun 25;8(12):2835–2842. [PMC free article] [PubMed]
  • Carvlin MJ, Fiel RJ. Intercalative and nonintercalative binding of large cationic porphyrin ligands to calf thymus DNA. Nucleic Acids Res. 1983 Sep 10;11(17):6121–6139. [PMC free article] [PubMed]
  • Carvlin MJ, Mark E, Fiel R, Howard JC. Intercalative and nonintercalative binding of large cationic porphyrin ligands to polynucleotides. Nucleic Acids Res. 1983 Sep 10;11(17):6141–6154. [PMC free article] [PubMed]
  • Pasternack RF, Gibbs EJ, Villafranca JJ. Interactions of porphyrins with nucleic acids. Biochemistry. 1983 May 10;22(10):2406–2414. [PubMed]
  • Kelly JM, Murphy MJ, McConnell DJ, OhUigin C. A comparative study of the interaction of 5,10,15,20-tetrakis (N-methylpyridinium-4-yl)porphyrin and its zinc complex with DNA using fluorescence spectroscopy and topoisomerisation. Nucleic Acids Res. 1985 Jan 11;13(1):167–184. [PMC free article] [PubMed]
  • Geacintov NE, Ibanez V, Rougee M, Bensasson RV. Orientation and linear dichroism characteristics of porphyrin-DNA complexes. Biochemistry. 1987 Jun 2;26(11):3087–3092. [PubMed]
  • Fiel RJ, Datta-Gupta N, Mark EH, Howard JC. Induction of DNA damage by porphyrin photosensitizers. Cancer Res. 1981 Sep;41(9 Pt 1):3543–3545. [PubMed]
  • Praseuth D, Gaudemer A, Verlhac JB, Kraljic I, Sissoëff I, Guillé E. Photocleavage of DNA in the presence of synthetic water-soluble porphyrins. Photochem Photobiol. 1986 Dec;44(6):717–724. [PubMed]
  • Fiel RJ, Beerman TA, Mark EH, Datta-Gupta N. DNA strand scission activity of metalloporphyrins. Biochem Biophys Res Commun. 1982 Aug;107(3):1067–1074. [PubMed]
  • Ford K, Fox KR, Neidle S, Waring MJ. DNA sequence preferences for an intercalating porphyrin compound revealed by footprinting. Nucleic Acids Res. 1987 Mar 11;15(5):2221–2234. [PMC free article] [PubMed]
  • Chinsky L, Turpin PY, Duquesne M. Resonance Raman study of actinomycin D interaction with DNA and its models. Biochem Biophys Res Commun. 1975 Aug 18;65(4):1440–1446. [PubMed]
  • Manfait M, Alix AJ, Jeannesson P, Jardillier JC, Theophanides T. Interaction of adriamycin with DNA as studied by resonance Raman spectroscopy. Nucleic Acids Res. 1982 Jun 25;10(12):3803–3816. [PMC free article] [PubMed]

Articles from Nucleic Acids Research are provided here courtesy of Oxford University Press

Formats:

Related citations in PubMed

See reviews...See all...

Cited by other articles in PMC

See all...

Links

  • Compound
    Compound
    PubChem Compound links
  • MedGen
    MedGen
    Related information in MedGen
  • PubMed
    PubMed
    PubMed citations for these articles
  • Substance
    Substance
    PubChem Substance links

Recent Activity

Your browsing activity is empty.

Activity recording is turned off.

Turn recording back on

See more...