• We are sorry, but NCBI web applications do not support your browser and may not function properly. More information
Logo of narLink to Publisher's site
Nucleic Acids Res. May 25, 1988; 16(10): 4341–4352.
PMCID: PMC336634

Site-specific degradation of Streptomyces lividans DNA during electrophoresis in buffers contaminated with ferrous iron.


Streptomyces lividans DNA contains a modification which makes it susceptible to double-strand cleavage during electrophoresis in buffers contaminated with ferrous iron (which may be present in some batches of EDTA). The cleavage of the DNA is site-specific and the average fragment size resulting from limit digestion of total S. lividans DNA is about 6kb. DNA from Streptomyces coelicolor A3(2) and several other Streptomyces strains, and from E. coli, is not cleaved under the same conditions. A S. lividans mutant has been isolated which lacks the DNA modification. We suspect that many reports of "poor" preparations of S. lividans plasmids may be due to the above effect.

Full text

Full text is available as a scanned copy of the original print version. Get a printable copy (PDF file) of the complete article (1.6M), or click on a page image below to browse page by page. Links to PubMed are also available for Selected References.

Images in this article

Selected References

These references are in PubMed. This may not be the complete list of references from this article.
  • Kieser T, Hopwood DA, Wright HM, Thompson CJ. pIJ101, a multi-copy broad host-range Streptomyces plasmid: functional analysis and development of DNA cloning vectors. Mol Gen Genet. 1982;185(2):223–228. [PubMed]
  • Hopwood DA, Kieser T, Wright HM, Bibb MJ. Plasmids, recombination and chromosome mapping in Streptomyces lividans 66. J Gen Microbiol. 1983 Jul;129(7):2257–2269. [PubMed]
  • Kieser T. Factors affecting the isolation of CCC DNA from Streptomyces lividans and Escherichia coli. Plasmid. 1984 Jul;12(1):19–36. [PubMed]
  • Tsai JF, Chen CW. Isolation and characterization of Streptomyces lividans mutants deficient in intraplasmid recombination. Mol Gen Genet. 1987 Jun;208(1-2):211–218. [PubMed]
  • Murray NE, Brammar WJ, Murray K. Lambdoid phages that simplify the recovery of in vitro recombinants. Mol Gen Genet. 1977 Jan 7;150(1):53–61. [PubMed]
  • Kieser T, Melton RE. Plasmid pIJ699, a multi-copy positive-selection vector for Streptomyces. Gene. 1988 May 15;65(1):83–91. [PubMed]
  • Maxam AM, Gilbert W. Sequencing end-labeled DNA with base-specific chemical cleavages. Methods Enzymol. 1980;65(1):499–560. [PubMed]
  • Chater KF, Wilde LC. Streptomyces albus G mutants defective in the SalGI restriction-modification system. J Gen Microbiol. 1980 Feb;116(2):323–334. [PubMed]
  • Kan NC, Lautenberger JA, Edgell MH, Hutchison CA., 3rd The nucleotide sequence recognized by the Escherichia coli K12 restriction and modification enzymes. J Mol Biol. 1979 May 15;130(2):191–209. [PubMed]
  • Kessler C, Höltke HJ. Specificity of restriction endonucleases and methylases--a review. Gene. 1986;47(1):1–153. [PubMed]
  • de Vries E, Bloemers SM, van der Vliet PC. Incorporation of 5-bromodeoxycytidine in the adenovirus 2 replication origin interferes with nuclear factor 1 binding. Nucleic Acids Res. 1987 Sep 25;15(18):7223–7234. [PMC free article] [PubMed]
  • Luckow B, Renkawitz R, Schütz G. A new method for constructing linker scanning mutants. Nucleic Acids Res. 1987 Jan 26;15(2):417–429. [PMC free article] [PubMed]
  • Altenbuchner J, Cullum J. DNA amplification and an unstable arginine gene in Streptomyces lividans 66. Mol Gen Genet. 1984;195(1-2):134–138. [PubMed]
  • Hertzberg RP, Dervan PB. Cleavage of DNA with methidiumpropyl-EDTA-iron(II): reaction conditions and product analyses. Biochemistry. 1984 Aug 14;23(17):3934–3945. [PubMed]
  • Dervan PB. Design of sequence-specific DNA-binding molecules. Science. 1986 Apr 25;232(4749):464–471. [PubMed]
  • Moser HE, Dervan PB. Sequence-specific cleavage of double helical DNA by triple helix formation. Science. 1987 Oct 30;238(4827):645–650. [PubMed]

Articles from Nucleic Acids Research are provided here courtesy of Oxford University Press


Related citations in PubMed

See reviews...See all...

Cited by other articles in PMC

See all...


Recent Activity

Your browsing activity is empty.

Activity recording is turned off.

Turn recording back on

See more...