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Nucleic Acids Res. Apr 25, 1988; 16(8): 3391–3403.
PMCID: PMC336501

Sequence differences upstream of the promoters are involved in the differential expression of the Xenopus somatic and oocyte 5S RNA genes.

Abstract

The Xenopus somatic and oocyte 5S RNA genes are differentially expressed in extracts of whole oocytes. In such extracts, sequence differences preceding the internal promoters significantly alter the relative activities of these genes. Following exchange of the sequences preceding the promoter, the activity of the somatic 5S gene decreased and that of the oocyte 5S gene increased. As a result, a 100 fold somatic transcriptional advantage was reduced to 5 fold. Analysis of deletion mutants showed that the relevant sequence differences are located between -34 and +37 relative to the initiation site. The observed transcriptional modulation is due both to sequence differences 5' to the initiation site and at positions 30 and 37 within the coding region.

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Selected References

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  • Wormington WM, Brown DD. Onset of 5 S RNA gene regulation during Xenopus embryogenesis. Dev Biol. 1983 Sep;99(1):248–257. [PubMed]
  • Brown DD, Schlissel MS. A positive transcription factor controls the differential expression of two 5S RNA genes. Cell. 1985 Oct;42(3):759–767. [PubMed]
  • Wolffe AP, Brown DD. Differential 5S RNA gene expression in vitro. Cell. 1987 Dec 4;51(5):733–740. [PubMed]
  • Peck LJ, Millstein L, Eversole-Cire P, Gottesfeld JM, Varshavsky A. Transcriptionally inactive oocyte-type 5S RNA genes of Xenopus laevis are complexed with TFIIIA in vitro. Mol Cell Biol. 1987 Oct;7(10):3503–3510. [PMC free article] [PubMed]
  • Wormington WM, Bogenhagen DF, Jordan E, Brown DD. A quantitative assay for Xenopus 5S RNA gene transcription in vitro. Cell. 1981 Jun;24(3):809–817. [PubMed]
  • Segall J, Matsui T, Roeder RG. Multiple factors are required for the accurate transcription of purified genes by RNA polymerase III. J Biol Chem. 1980 Dec 25;255(24):11986–11991. [PubMed]
  • Shastry BS, Ng SY, Roeder RG. Multiple factors involved in the transcription of class III genes in Xenopus laevis. J Biol Chem. 1982 Nov 10;257(21):12979–12986. [PubMed]
  • Engelke DR, Ng SY, Shastry BS, Roeder RG. Specific interaction of a purified transcription factor with an internal control region of 5S RNA genes. Cell. 1980 Mar;19(3):717–728. [PubMed]
  • Lassar AB, Martin PL, Roeder RG. Transcription of class III genes: formation of preinitiation complexes. Science. 1983 Nov 18;222(4625):740–748. [PubMed]
  • Setzer DR, Brown DD. Formation and stability of the 5 S RNA transcription complex. J Biol Chem. 1985 Feb 25;260(4):2483–2492. [PubMed]
  • Bogenhagen DF, Wormington WM, Brown DD. Stable transcription complexes of Xenopus 5S RNA genes: a means to maintain the differentiated state. Cell. 1982 Feb;28(2):413–421. [PubMed]
  • Pieler T, Hamm J, Roeder RG. The 5S gene internal control region is composed of three distinct sequence elements, organized as two functional domains with variable spacing. Cell. 1987 Jan 16;48(1):91–100. [PubMed]
  • Bogenhagen DF, Sakonju S, Brown DD. A control region in the center of the 5S RNA gene directs specific initiation of transcription: II. The 3' border of the region. Cell. 1980 Jan;19(1):27–35. [PubMed]
  • Sakonju S, Bogenhagen DF, Brown DD. A control region in the center of the 5S RNA gene directs specific initiation of transcription: I. The 5' border of the region. Cell. 1980 Jan;19(1):13–25. [PubMed]
  • Peterson RC, Doering JL, Brown DD. Characterization of two xenopus somatic 5S DNAs and one minor oocyte-specific 5S DNA. Cell. 1980 May;20(1):131–141. [PubMed]
  • Fedoroff NV, Brown DD. The nucleotide sequence of oocyte 5S DNA in Xenopus laevis. I. The AT-rich spacer. Cell. 1978 Apr;13(4):701–716. [PubMed]
  • Ng SY, Parker CS, Roeder RG. Transcription of cloned Xenopus 5S RNA genes by X. laevis RNA polymerase III in reconstituted systems. Proc Natl Acad Sci U S A. 1979 Jan;76(1):136–140. [PMC free article] [PubMed]
  • Wakefield L, Gurdon JB. Cytoplasmic regulation of 5S RNA genes in nuclear-transplant embryos. EMBO J. 1983;2(9):1613–1619. [PMC free article] [PubMed]
  • Sakonju S, Brown DD, Engelke D, Ng SY, Shastry BS, Roeder RG. The binding of a transcription factor to deletion mutants of a 5S ribosomal RNA gene. Cell. 1981 Mar;23(3):665–669. [PubMed]
  • Ullu E, Weiner AM. Upstream sequences modulate the internal promoter of the human 7SL RNA gene. Nature. 318(6044):371–374. [PubMed]
  • Korn LJ. Transcription of Xenopus 5S ribosomal RNA genes. Nature. 1982 Jan 14;295(5845):101–105. [PubMed]
  • Garcia AD, O'Connell AM, Sharp SJ. Formation of an active transcription complex in the Drosophila melanogaster 5S RNA gene is dependent on an upstream region. Mol Cell Biol. 1987 Jun;7(6):2046–2051. [PMC free article] [PubMed]
  • Morton DG, Sprague KU. In vitro transcription of a silkworm 5S RNA gene requires an upstream signal. Proc Natl Acad Sci U S A. 1984 Sep;81(17):5519–5522. [PMC free article] [PubMed]
  • Selker EU, Morzycka-Wroblewska E, Stevens JN, Metzenberg RL. An upstream signal is required for in vitro transcription of Neurospora 5S RNA genes. Mol Gen Genet. 1986 Oct;205(1):189–192. [PubMed]

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