• We are sorry, but NCBI web applications do not support your browser and may not function properly. More information
Logo of narLink to Publisher's site
Nucleic Acids Res. Mar 25, 1988; 16(6): 2489–2507.
PMCID: PMC336385

Sequence-specific binding of luzopeptin to DNA.

Abstract

We have examined the binding of luzopeptin, an antitumor antibiotic, to five DNA fragments of varying base composition. The drug forms a tight, possibly covalent, complex with the DNA causing a reduction in mobility on nondenaturing polyacrylamide gels and some smearing of the bands consistent with intramolecular cross-linking of DNA duplexes. DNAase I and micrococcal nuclease footprinting experiments suggest that the drug binds best to regions containing alternating A and T residues, although no consensus di- or trinucleotide sequence emerges. Binding to other sites is not excluded and at moderate ligand concentrations the DNA is almost totally protected from enzyme attack. Ligand-induced enhancement of DNAase I cleavage is observed at both AT and GC-rich regions. The sequence selectivity and characteristics of luzopeptin binding are quite different from those of echinomycin, a bifunctional intercalator of related structure.

Full text

Full text is available as a scanned copy of the original print version. Get a printable copy (PDF file) of the complete article (1.9M), or click on a page image below to browse page by page. Links to PubMed are also available for Selected References.

Images in this article

Selected References

These references are in PubMed. This may not be the complete list of references from this article.
  • Huang CH, Mong S, Crooke ST. Interactions of a new antitumor antibiotic BBM-928A with deoxyribonucleic acid. Bifunctional intercalative binding studied by fluorometry and viscometry. Biochemistry. 1980 Nov 25;19(24):5537–5542. [PubMed]
  • Ohkuma H, Sakai F, Nishiyama Y, Ohbayashi M, Imanishi H, Konishi M, Miyaki T, Koshiyama H, Kawaguchi H. BBM-928, a new antitumor antibiotic complex. I. Production, isolation, characterization and antitumor activity. J Antibiot (Tokyo) 1980 Oct;33(10):1087–1097. [PubMed]
  • Huang CH, Prestayko AW, Crooke ST. Bifunctional intercalation of antitumor antibiotics BBM-928A and echinomycin with deoxyribonucleic acid. Effects of intercalation on deoxyribonucleic acid degradative activity of bleomycin and phleomycin. Biochemistry. 1982 Jul 20;21(15):3704–3710. [PubMed]
  • Huang CH, Mirabelli CK, Mong S, Crooke ST. Intermolecular cross-linking of DNA through bifunctional intercalation of an antitumor antibiotic, luzopeptin A (BBM-928A). Cancer Res. 1983 Jun;43(6):2718–2724. [PubMed]
  • Ughetto G, Wang AH, Quigley GJ, van der Marel GA, van Boom JH, Rich A. A comparison of the structure of echinomycin and triostin A complexed to a DNA fragment. Nucleic Acids Res. 1985 Apr 11;13(7):2305–2323. [PMC free article] [PubMed]
  • Wakelin SP, Waring MJ. The binding of echinomycin to deoxyribonucleic acid. Biochem J. 1976 Sep 1;157(3):721–740. [PMC free article] [PubMed]
  • Van Dyke MM, Dervan PB. Echinomycin binding sites on DNA. Science. 1984 Sep 14;225(4667):1122–1127. [PubMed]
  • Low CM, Drew HR, Waring MJ. Sequence-specific binding of echinomycin to DNA: evidence for conformational changes affecting flanking sequences. Nucleic Acids Res. 1984 Jun 25;12(12):4865–4879. [PMC free article] [PubMed]
  • Low CM, Fox KR, Olsen RK, Waring MJ. DNA sequence recognition by under-methylated analogues of triostin A. Nucleic Acids Res. 1986 Mar 11;14(5):2015–2033. [PMC free article] [PubMed]
  • Fox KR, Waring MJ. Nucleotide sequence binding preferences of nogalamycin investigated by DNase I footprinting. Biochemistry. 1986 Jul 29;25(15):4349–4356. [PubMed]
  • Peterson RC, Doering JL, Brown DD. Characterization of two xenopus somatic 5S DNAs and one minor oocyte-specific 5S DNA. Cell. 1980 May;20(1):131–141. [PubMed]
  • Fox KR, Waring MJ. The use of micrococcal nuclease as a probe for drug-binding sites on DNA. Biochim Biophys Acta. 1987 Jul 14;909(2):145–155. [PubMed]
  • Fox KR, Waring MJ. DNA structural variations produced by actinomycin and distamycin as revealed by DNAase I footprinting. Nucleic Acids Res. 1984 Dec 21;12(24):9271–9285. [PMC free article] [PubMed]

Articles from Nucleic Acids Research are provided here courtesy of Oxford University Press

Formats:

Related citations in PubMed

See reviews...See all...

Cited by other articles in PMC

See all...

Links

  • Compound
    Compound
    PubChem Compound links
  • MedGen
    MedGen
    Related information in MedGen
  • PubMed
    PubMed
    PubMed citations for these articles
  • Substance
    Substance
    PubChem Substance links

Recent Activity

Your browsing activity is empty.

Activity recording is turned off.

Turn recording back on

See more...