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Proc Natl Acad Sci U S A. Oct 1978; 75(10): 4906–4910.
PMCID: PMC336230

Effect of anesthetics and pressure on the thermotropic behavior of multilamellar dipalmitoylphosphatidylcholine liposomes.

Abstract

The effects of gaseous anesthetics and pressure on the thermotropic behavior of multilamellar dipalmitoyl-phosphatidylcholine liposomes were studied by using a high-sensitivity differential scanning calorimeter. It was found that halothane and enflurane decreased the transition temperature and increased the width of the transition without affecting the enthalpy change for the main gel-to-liquid crystalline transition. This demonstrated that the anesthetics decreased the degree of cooperative interaction between phospholipid molecules within the bilayer. Increasing the pressure increased the transition temperature but did not affect the transition width or enthalpy change. However, the increase in pressure reversed the effect of anesthetic on both the transition temperature and transition width. It is suggested that an understanding of the effect of anesthetics on the degree of cooperative interaction between phospholipids may be a key to understanding anesthetic action.

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Selected References

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  • Saidman LJ, Eger EI, 2nd, Munson ES, Babad AA, Muallem M. Minimum alveolar concentrations of methoxyflurane, halothane, ether and cyclopropane in man: correlation with theories of anesthesia. Anesthesiology. 1967 Nov-Dec;28(6):994–1002. [PubMed]
  • Trudell JR, Payan DG, Chin JH, Cohen EN. The antagonistic effect of an inhalation anesthetic and high pressure on the phase diagram of mixed dipalmitoyl-dimyristoylphosphatidylcholine bilayers. Proc Natl Acad Sci U S A. 1975 Jan;72(1):210–213. [PMC free article] [PubMed]
  • Lever MJ, Miller KW, Paton WD, Smith EB. Pressure reversal of anaesthesia. Nature. 1971 Jun 11;231(5302):368–371. [PubMed]
  • Trudell JR. A unitary theory of anesthesia based on lateral phase separations in nerve membranes. Anesthesiology. 1977 Jan;46(1):5–10. [PubMed]
  • Suurkuusk J, Lentz BR, Barenholz Y, Biltonen RL, Thompson TE. A calorimetric and fluorescent probe study of the gel-liquid crystalline phase transition in small, single-lamellar dipalmitoylphosphatidylcholine vesicles. Biochemistry. 1976 Apr 6;15(7):1393–1401. [PubMed]
  • Eger EI, 2nd, Saidman LJ, Brandstater B. Temperature dependence of halothane and cyclopropane anesthesia in dogs: correlation with some theories of anesthetic action. Anesthesiology. 1965 Nov-Dec;26(6):764–770. [PubMed]
  • Mabrey S, Sturtevant JM. Investigation of phase transitions of lipids and lipid mixtures by sensitivity differential scanning calorimetry. Proc Natl Acad Sci U S A. 1976 Nov;73(11):3862–3866. [PMC free article] [PubMed]
  • Janiak MJ, Small DM, Shipley GG. Nature of the Thermal pretransition of synthetic phospholipids: dimyristolyl- and dipalmitoyllecithin. Biochemistry. 1976 Oct 19;15(21):4575–4580. [PubMed]
  • Papahadjopoulos D, Jacobson K, Poste G, Shepherd G. Effects of local anesthetics on membrane properties. I. Changes in the fluidity of phospholipid bilayers. Biochim Biophys Acta. 1975 Jul 18;394(4):504–519. [PubMed]
  • Tsong TY, Kanehisa MI. Relaxation phenomena in aqueous dispersions of synthetic lecithins. Biochemistry. 1977 Jun 14;16(12):2674–2680. [PubMed]
  • Hill MW. The effect of anaesthetic-like molecules on the phase transition in smectic mesophases of dipalmitoyllecithin. I. The normal alcohol up to C equals 9 and three inhalation anaesthetics. Biochim Biophys Acta. 1974 Jul 12;356(1):117–124. [PubMed]
  • Forsyth PA, Jr, Marcelja S, Mitchell DJ, Ninham BW. Phase transition in charged lipid membranes. Biochim Biophys Acta. 1977 Sep 19;469(3):335–344. [PubMed]
  • Tsong TY, Greenberg M, Kanehisa MI. Anesthetic action of membrane lipids. Biochemistry. 1977 Jul 12;16(14):3115–3121. [PubMed]
  • Lee AG. Model for action of local anaesthetics. Nature. 1976 Aug 12;262(5569):545–548. [PubMed]

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