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Proc Natl Acad Sci U S A. Oct 1978; 75(10): 4779–4783.
PMCID: PMC336203

Symmetry mismatch and DNA packaging in large bacteriophages.


A model is presented for the mechanism of packaging double-stranded DNA into phage heads. The model is based on, and rationalizes, the mismatch in symmetry between the heads and tails of large bacteriophages. DNA movement is postulated to be mediated by a rotating protein structure at the tail-proximal vertex of the head.

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  • Moody MF. The shape of the T-even bacteriophage head. Virology. 1965 Aug;26(4):567–576. [PubMed]
  • CASPAR DL, KLUG A. Physical principles in the construction of regular viruses. Cold Spring Harb Symp Quant Biol. 1962;27:1–24. [PubMed]
  • Crowther RA, Klug A. Structural analysis of macromolecular assemblies by image reconstruction from electron micrographs. Annu Rev Biochem. 1975;44:161–182. [PubMed]
  • Serwer P. Internal proteins of bacteriophage T7. J Mol Biol. 1976 Nov 5;107(3):271–291. [PubMed]
  • Anderson DL, Hickman DD, Reilly BE. Structure of Bacillus subtilis bacteriophage phi 29 and the length of phi 29 deoxyribonucleic acid. J Bacteriol. 1966 May;91(5):2081–2089. [PMC free article] [PubMed]
  • Casjens S, King J. Virus assembly. Annu Rev Biochem. 1975;44:555–611. [PubMed]
  • Mazza A, Felluga B. Electron microscope observations on lambda bacteriophage ultrastructure. J Ultrastruct Res. 1973 Nov;45(3):259–278. [PubMed]
  • Coombs DH, Eiserling FA. Studies on the structure, protein composition and aseembly of the neck of bacteriophage T4. J Mol Biol. 1977 Nov 5;116(3):375–405. [PubMed]
  • Cummings DJ, Chapman VA, DeLong SS. Disruption of T-even bacteriophages by dimethyl sulfoxide. J Virol. 1968 Jun;2(6):610–620. [PMC free article] [PubMed]
  • Yanagida M, Ahmad-Zadeh C. Determination of gene product positions in bacteriophage T4 by specific antibody association. J Mol Biol. 1970 Jul 28;51(2):411–421. [PubMed]
  • Bassel A, Shaw M, Campbell LL. Dissociation by chelating agents and substructure of the thermophilic bacteriophage TP84. J Virol. 1971 May;7(5):663–672. [PMC free article] [PubMed]
  • Harrison DP, Brown DT, Bode VC. The lambda head-tail joining reaction: purification, properties and structure of biologically active heads and tails. J Mol Biol. 1973 Sep 25;79(3):437–449. [PubMed]
  • Paulson JR, Laemmli UK. Morphogenetic core of the bacteriophage T4 head. Structure of the core in polyheads. J Mol Biol. 1977 Apr 25;111(4):459–485. [PubMed]
  • Hohn T, Flick H, Hohn B. Petit lambda, a family of particles from coliphage lambda infected cells. J Mol Biol. 1975 Oct 15;98(1):107–120. [PubMed]
  • Casjens SR, Hendrix RW. Locations and amounts of major structural proteins in bacteriophage lambda. J Mol Biol. 1974 Sep 15;88(2):535–545. [PubMed]
  • Hagen EW, Reilly BE, Tosi ME, Anderson DL. Analysis of gene function of bacteriophage phi 29 of Bacillus subtilis: identification of cistrons essential for viral assembly. J Virol. 1976 Aug;19(2):501–517. [PMC free article] [PubMed]
  • Casjens S, King J. P22 morphogenesis. I: Catalytic scaffolding protein in capsid assembly. J Supramol Struct. 1974;2(2-4):202–224. [PubMed]
  • Earnshaw W, Casjens S, Harrison SC. Assembly of the head of bacteriophage P22: x-ray diffraction from heads, proheads and related structures. J Mol Biol. 1976 Jun 25;104(2):387–410. [PubMed]
  • Earnshaw WC, Harrison SC. DNA arrangement in isometric phage heads. Nature. 1977 Aug 18;268(5621):598–602. [PubMed]
  • Brody T. A DNA-binding form of the main structural protein of lambda heads. Virology. 1973 Aug;54(2):441–451. [PubMed]
  • Kerr C, Sadowski PD. Packaging and maturation of DNA of bacteriophage T7 in vitro. Proc Natl Acad Sci U S A. 1974 Sep;71(9):3545–3549. [PMC free article] [PubMed]
  • Becker A, Murialdo H, Gold M. Studies on an in vitro system for the packaging and maturation of phage lambda DNA. Virology. 1977 May 1;78(1):277–290. [PubMed]
  • Hohn B, Hohn T. Activity of empty, headlike particles for packaging of DNA of bacteriophage lambda in vitro. Proc Natl Acad Sci U S A. 1974 Jun;71(6):2372–2376. [PMC free article] [PubMed]
  • Pruss G, Goldstein RN, Calendar R. In vitro packaging of satellite phage P4 DNA. Proc Natl Acad Sci U S A. 1974 Jun;71(6):2367–2371. [PMC free article] [PubMed]
  • Kaiser D, Syvanen M, Masuda T. DNA packaging steps in bacteriophage lambda head assembly. J Mol Biol. 1975 Jan 15;91(2):175–186. [PubMed]
  • Becker A, Marko M, Gold M. Early events in the in vitro packaging of bacteriophage lambda DNA. Virology. 1977 May 1;78(1):291–305. [PubMed]
  • Manor H, Goodman D, Stent GS. RNA chain growth rates in Escherichia coli. J Mol Biol. 1969 Jan 14;39(1):1–29. [PubMed]
  • Helmstetter CE. DNA synthesis during the division cycle of rapidly growing Escherichia coli B/r. J Mol Biol. 1968 Feb 14;31(3):507–518. [PubMed]
  • Bachmann BJ, Low KB, Taylor AL. Recalibrated linkage map of Escherichia coli K-12. Bacteriol Rev. 1976 Mar;40(1):116–167. [PMC free article] [PubMed]
  • Padmanabhan R, Wu R, Bode VC. Arrangement of DNA in lambda bacteriophage heads. 3. Location and number of nucleotides cleaved from lambda-DNA by micrococcal nuclease attack on heads. J Mol Biol. 1972 Aug 21;69(2):201–207. [PubMed]
  • Saigo K, Uchida H. Connection of the right-hand terminus of DNA to the proximal end of the tail in bacteriophage lambda. Virology. 1974 Oct;61(2):524–536. [PubMed]
  • Thomas JO. Chemical linkage of the tail to the right-hand end of bacteriophage lambda DNA. J Mol Biol. 1974 Jul 25;87(1):1–9. [PubMed]
  • Chattoraj DK, Inman RB. Location of DNA ends in P2, 186, P4 and lambda bacteriophage heads. J Mol Biol. 1974 Jul 25;87(1):11–22. [PubMed]
  • Laemmli UK, Paulson JR, Hitchins V. Maturation of the head of bacteriophage T4. V. A possible DNA packaging mechanism: in vitro cleavage of the head proteins and the structure of the core of the polyhead. J Supramol Struct. 1974;2(2-4):276–301. [PubMed]
  • Hohn B, Wurtz M, Klein B, Lustig A, Hohn T. Phage lambda DNA packaging, in vitro. J Supramol Struct. 1974;2(2-4):302–317. [PubMed]
  • Serwer P. Buoyant density sedimentation of macromolecules in sodium iothalamate density gradients. J Mol Biol. 1975 Mar 5;92(3):433–448. [PubMed]
  • Hendrix RW, Casjens SR. Assembly of bacteriophage lambda heads: protein processing and its genetic control in petit lambda assembly. J Mol Biol. 1975 Jan 15;91(2):187–199. [PubMed]
  • Kaiser D, Masuda T. In vitro assembly of bacteriophage Lambda heads. Proc Natl Acad Sci U S A. 1973 Jan;70(1):260–264. [PMC free article] [PubMed]
  • Zachary A, Simon LD, Litwin S. Lambda head morphogenesis as seen in the electron microscope. Virology. 1976 Jul 15;72(2):429–442. [PubMed]
  • Lickfeld KG, Menge B. Morphogenesis of bacteriophage lambda: electron microscopy of thin sections. J Mol Biol. 1976 May 15;103(2):299–318. [PubMed]
  • Hsiao CL, Black LW. DNA packaging and the pathway of bacteriophage T4 head assembly. Proc Natl Acad Sci U S A. 1977 Sep;74(9):3652–3656. [PMC free article] [PubMed]
  • Müller-Salamin L, Onorato L, Showe MK. Localization of minor protein components of the head of bacteriophage T4. J Virol. 1977 Oct;24(1):121–134. [PMC free article] [PubMed]
  • Streisinger G, Emrich J, Stahl MM. Chromosome structure in phage t4, iii. Terminal redundancy and length determination. Proc Natl Acad Sci U S A. 1967 Feb;57(2):292–295. [PMC free article] [PubMed]
  • Berg HC, Anderson RA. Bacteria swim by rotating their flagellar filaments. Nature. 1973 Oct 19;245(5425):380–382. [PubMed]
  • Silverman M, Simon M. Flagellar rotation and the mechanism of bacterial motility. Nature. 1974 May 3;249(452):73–74. [PubMed]

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