Logo of pnasPNASInfo for AuthorsSubscriptionsAboutThis Article
Proc Natl Acad Sci U S A. Sep 1978; 75(9): 4384–4388.
PMCID: PMC336119

Rate of macromolecular synthesis through the cell cycle of the yeast Saccharomyces cerevisiae.


Centrifugal elutriation was used to separate cells of Saccharomyces cerevisiae in balanced exponential growth according to position in the cell cycle. Macromolecular synthesis was examined. DNA synthesis was found to be periodic, but RNA and protein synthesis showed an exponential increase in rate. Two-dimensional electrophoresis was used to determine the rate of synthesis of individual proteins, with 111 of the more abundant cellular proteins selected for analysis from among the more than 1000 proteins that migrate in the system. All the examined proteins showed an exponentially increasing rate of synthesis.

Full text

Full text is available as a scanned copy of the original print version. Get a printable copy (PDF file) of the complete article (1.2M), or click on a page image below to browse page by page. Links to PubMed are also available for Selected References.

Images in this article

Click on the image to see a larger version.

Selected References

These references are in PubMed. This may not be the complete list of references from this article.
  • Mitchison JM. Enzyme synthesis in synchronous cultures. Science. 1969 Aug 15;165(3894):657–663. [PubMed]
  • Halvorson HO, Carter BL, Tauro P. Synthesis of enzymes during the cell cycle. Adv Microb Physiol. 1971;6(0):47–106. [PubMed]
  • Mitchison JM, Creanor J. Linear synthesis of sucrase and phosphatases during the cell cycle of Schizosaccharomyces pombe. J Cell Sci. 1969 Sep;5(2):373–391. [PubMed]
  • Wain WH. Synthesis of soluble protein during the cell cycle of the fission yeast Schizosaccharomyces pombe. Exp Cell Res. 1971 Nov;69(1):49–56. [PubMed]
  • Shulman RW, Hartwell LH, Warner JR. Synthesis of ribosomal proteins during the yeast cell cycle. J Mol Biol. 1973 Feb 5;73(4):513–525. [PubMed]
  • Gordon CN, Elliott SC. Fractionation of Saccharomyces cerevisiae cell populations by centrifugal elutriation. J Bacteriol. 1977 Jan;129(1):97–100. [PMC free article] [PubMed]
  • O'Farrell PH. High resolution two-dimensional electrophoresis of proteins. J Biol Chem. 1975 May 25;250(10):4007–4021. [PMC free article] [PubMed]
  • Graham R, Stanley WM., Jr An economical procedure for the preparation of L-( 35 S)methionine of high specific activity. Anal Biochem. 1972 Jun;47(2):505–513. [PubMed]
  • Simchen G, Piñon R, Salts Y. Sporulation in Saccharomyces cerevisiae: premeiotic DNA synthesis, readiness and commitment. Exp Cell Res. 1972 Nov;75(1):207–218. [PubMed]
  • Hartwell LH. Periodic density fluctuation during the yeast cell cycle and the selection of synchronous cultures. J Bacteriol. 1970 Dec;104(3):1280–1285. [PMC free article] [PubMed]
  • Irie R, Yano N, Morichi T, Kembo H. Temporary increase in UV-resistance in the course of spore germination of Bacillus subtilis. Biochem Biophys Res Commun. 1965 Aug 16;20(4):389–392. [PubMed]
  • Culotti J, Hartwell LH. Genetic control of the cell division cycle in yeast. 3. Seven genes controlling nuclear division. Exp Cell Res. 1971 Aug;67(2):389–401. [PubMed]
  • Hartwell LH. Genetic control of the cell division cycle in yeast. IV. Genes controlling bud emergence and cytokinesis. Exp Cell Res. 1971 Dec;69(2):265–276. [PubMed]
  • Hartwell LH, Culotti J, Pringle JR, Reid BJ. Genetic control of the cell division cycle in yeast. Science. 1974 Jan 11;183(4120):46–51. [PubMed]
  • CAMPBELL A. Synchronization of cell division. Bacteriol Rev. 1957 Dec;21(4):263–272. [PMC free article] [PubMed]
  • Sebastian J, Carter BL, Halvorson HO. Use of yeast populations fractionated by zonal centrifugation to study the cell cycle. J Bacteriol. 1971 Dec;108(3):1045–1050. [PMC free article] [PubMed]
  • Sogin SJ, Carter BL, Halvorson HO. Changes in the rate of ribosomal RNA synthesis during the cell cycle in Saccharomyces cerevisiae. Exp Cell Res. 1974 Nov;89(1):127–138. [PubMed]
  • Fraser RS, Carter BL. Synthesis of polyadenylated messenger RNA during the cell cycle of Saccharomyces cerevisiae. J Mol Biol. 1976 Jun 14;104(1):223–242. [PubMed]
  • Fraser RS, Moreno F. Rates of synthesis of polyadenylated messenger RNA and ribosomal RNA during the cell cycle of Schizosaccharomyces pombe. With an appendix: calculation of the pattern of protein accumulation from observed changes in the rate of messenger RNA synthesis. J Cell Sci. 1976 Aug;21(3):497–521. [PubMed]
  • Warner JR, Morgan SA, Shulman RW. Kinetics of labeling of the S-adenosylmethionine pool of Saccharomyces cerevisiae. J Bacteriol. 1976 Mar;125(3):887–891. [PMC free article] [PubMed]
  • Gorman J, Taruo P, LaBerge M, Halvorson H. Timing of enzyme synthesis during synchronous division in yeast. Biochem Biophys Res Commun. 1964 Feb 18;15(1):43–49. [PubMed]

Articles from Proceedings of the National Academy of Sciences of the United States of America are provided here courtesy of National Academy of Sciences


Related citations in PubMed

See reviews...See all...

Cited by other articles in PMC

See all...


Recent Activity

Your browsing activity is empty.

Activity recording is turned off.

Turn recording back on

See more...