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Proc Natl Acad Sci U S A. Feb 1976; 73(2): 327–330.
PMCID: PMC335900

5'-terminal structure of poliovirus polyribosomal RNA is pUp.

Abstract

Poliovirus RNA purified from virus-specific polyribosomes does not contain m7G in a 5'-5'-pyrophosphate linkage at its 5'-end. The only potential 5'-end found in ribonuclease digests of this RNA is pUp, which is present in a yield of 1 mole/mole of poliovirus RNA. We conclude that a 5'-terminal m7G is not required for translation of at least one RNA species in animal cells.

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Selected References

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  • Furuichi Y, Morgan M, Muthukrishnan S, Shatkin AJ. Reovirus messenger RNA contains a methylated, blocked 5'-terminal structure: m-7G(5')ppp(5')G-MpCp-. Proc Natl Acad Sci U S A. 1975 Jan;72(1):362–366. [PMC free article] [PubMed]
  • Abraham G, Rhodes DP, Banerjee AK. The 5' terminal structure of the methylated mRNA synthesized in vitro by vesicular stomatitis virus. Cell. 1975 May;5(1):51–58. [PubMed]
  • Wei CM, Moss B. Methylated nucleotides block 5'-terminus of vaccinia virus messenger RNA. Proc Natl Acad Sci U S A. 1975 Jan;72(1):318–322. [PMC free article] [PubMed]
  • Furuichi Y, Muthukrishnan S, Shatkin AJ. 5'-Terminal m-7G(5')ppp(5')G-m-p in vivo: identification in reovirus genome RNA. Proc Natl Acad Sci U S A. 1975 Feb;72(2):742–745. [PMC free article] [PubMed]
  • Rose JK. Heterogneeous 5'-terminal structures occur on vesicular stomatitis virus mRNAs. J Biol Chem. 1975 Oct 25;250(20):8098–8104. [PubMed]
  • Moyer SA, Abraham G, Adler R, Banerjee AK. Methylated and blocked 5' termini in vesicular stomatitis virus in vivo mRNAs. Cell. 1975 May;5(1):59–67. [PubMed]
  • Both GW, Banerjee AK, Shatkin AJ. Methylation-dependent translation of viral messenger RNAs in vitro. Proc Natl Acad Sci U S A. 1975 Mar;72(3):1189–1193. [PMC free article] [PubMed]
  • Muthukrishnan S, Both GW, Furuichi Y, Shatkin AJ. 5'-Terminal 7-methylguanosine in eukaryotic mRNA is required for translation. Nature. 1975 May 1;255(5503):33–37. [PubMed]
  • Wimmer E. Sequence studies of poliovirus RNA. I. Characterization of the 5'-terminus. J Mol Biol. 1972 Jul 28;68(3):537–540. [PubMed]
  • Villa-Komaroff L, Guttman N, Baltimore D, Lodishi HF. Complete translation of poliovirus RNA in a eukaryotic cell-free system. Proc Natl Acad Sci U S A. 1975 Oct;72(10):4157–4161. [PMC free article] [PubMed]
  • Baltimore D, Girard M, Darnell JE. Aspects of the synthesis of poliovirus RNA and the formation of virus particles. Virology. 1966 Jun;29(2):179–189. [PubMed]
  • Spector DH, Baltimore D. Polyadenylic acid on poliovirus RNA. II. poly(A) on intracellular RNAs. J Virol. 1975 Jun;15(6):1418–1431. [PMC free article] [PubMed]
  • Walker RT, RajBhandary UL. Formylatable methionine transfer RNA from Mycoplasma: purification and comparison of partial nucleotide sequences with those of other prokaryotic initiator tRNAs. Nucleic Acids Res. 1975 Jan;2(1):61–78. [PMC free article] [PubMed]
  • Granboulan N, Girard M. Molecular weight of poliovirus ribonucleic acid. J Virol. 1969 Oct;4(4):475–479. [PMC free article] [PubMed]
  • Nomoto A, Lee YF, Wimmer E. The 5' end of poliovirus mRNA is not capped with m7G(5')ppp(5')Np. Proc Natl Acad Sci U S A. 1976 Feb;73(2):375–380. [PMC free article] [PubMed]
  • Lawrence C, Thach RE. Encephalomyocarditis virus infection of mouse plasmacytoma cells. I. Inhibition of cellular protein synthesis. J Virol. 1974 Sep;14(3):598–610. [PMC free article] [PubMed]

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