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Nucleic Acids Res. Oct 11, 1989; 17(19): 7623–7630.
PMCID: PMC334871

Polymers of random short oligonucleotides detect polymorphic loci in the human genome.

Abstract

Polymers of random 14 mer oligonucleotides are shown to detect discrete loci in the human genome. Eighteen different synthetic tandem repeats of random 14 base-pair units (STRs) have been generated and all of them turn out to detect polymorphic loci on southern blots of human DNA samples, presumably corresponding to a variable number of tandem repeats (VNTR). This finding suggests that minisatellites are a major component of the human genome and are strongly associated with the generation of genetic variability. In addition, it should open new strategies to make new polymorphic probes available.

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Selected References

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  • Jeffreys AJ, Wilson V, Thein SL. Hypervariable 'minisatellite' regions in human DNA. Nature. 1985 Mar 7;314(6006):67–73. [PubMed]
  • Wyman AR, White R. A highly polymorphic locus in human DNA. Proc Natl Acad Sci U S A. 1980 Nov;77(11):6754–6758. [PMC free article] [PubMed]
  • Nakamura Y, Leppert M, O'Connell P, Wolff R, Holm T, Culver M, Martin C, Fujimoto E, Hoff M, Kumlin E, et al. Variable number of tandem repeat (VNTR) markers for human gene mapping. Science. 1987 Mar 27;235(4796):1616–1622. [PubMed]
  • Tautz D, Trick M, Dover GA. Cryptic simplicity in DNA is a major source of genetic variation. Nature. 1986 Aug 14;322(6080):652–656. [PubMed]
  • Smith GP. Unequal crossover and the evolution of multigene families. Cold Spring Harb Symp Quant Biol. 1974;38:507–513. [PubMed]
  • Chung CT, Miller RH. A rapid and convenient method for the preparation and storage of competent bacterial cells. Nucleic Acids Res. 1988 Apr 25;16(8):3580–3580. [PMC free article] [PubMed]
  • Del Sal G, Manfioletti G, Schneider C. A one-tube plasmid DNA mini-preparation suitable for sequencing. Nucleic Acids Res. 1988 Oct 25;16(20):9878–9878. [PMC free article] [PubMed]
  • Church GM, Gilbert W. Genomic sequencing. Proc Natl Acad Sci U S A. 1984 Apr;81(7):1991–1995. [PMC free article] [PubMed]
  • Feinberg AP, Vogelstein B. A technique for radiolabeling DNA restriction endonuclease fragments to high specific activity. Anal Biochem. 1983 Jul 1;132(1):6–13. [PubMed]
  • Meinkoth J, Wahl G. Hybridization of nucleic acids immobilized on solid supports. Anal Biochem. 1984 May 1;138(2):267–284. [PubMed]
  • Schumm JW, Knowlton RG, Braman JC, Barker DF, Botstein D, Akots G, Brown VA, Gravius TC, Helms C, Hsiao K, et al. Identification of more than 500 RFLPs by screening random genomic clones. Am J Hum Genet. 1988 Jan;42(1):143–159. [PMC free article] [PubMed]
  • Pearson PL, Kidd KK, Willard HF. Report of the committee on human gene mapping by recombinant DNA techniques. Cytogenet Cell Genet. 1987;46(1-4):390–566. [PubMed]
  • Kidd KK, Bowcock AM, Pearson PL, Schmidtke J, Willard HF, Track RK, Ricciuti F. Report of the committee on human gene mapping by recombinant DNA techniques. Cytogenet Cell Genet. 1988;49(1-3):132–218. [PubMed]
  • Wong Z, Wilson V, Jeffreys AJ, Thein SL. Cloning a selected fragment from a human DNA 'fingerprint': isolation of an extremely polymorphic minisatellite. Nucleic Acids Res. 1986 Jun 11;14(11):4605–4616. [PMC free article] [PubMed]
  • Nakamura Y, Carlson M, Krapcho K, Kanamori M, White R. New approach for isolation of VNTR markers. Am J Hum Genet. 1988 Dec;43(6):854–859. [PMC free article] [PubMed]
  • Weber JL, May PE. Abundant class of human DNA polymorphisms which can be typed using the polymerase chain reaction. Am J Hum Genet. 1989 Mar;44(3):388–396. [PMC free article] [PubMed]
  • Litt M, Luty JA. A hypervariable microsatellite revealed by in vitro amplification of a dinucleotide repeat within the cardiac muscle actin gene. Am J Hum Genet. 1989 Mar;44(3):397–401. [PMC free article] [PubMed]
  • Yu Q, Colot HV, Kyriacou CP, Hall JC, Rosbash M. Behaviour modification by in vitro mutagenesis of a variable region within the period gene of Drosophila. Nature. 1987 Apr 23;326(6115):765–769. [PubMed]
  • Koenig M, Monaco AP, Kunkel LM. The complete sequence of dystrophin predicts a rod-shaped cytoskeletal protein. Cell. 1988 Apr 22;53(2):219–228. [PubMed]
  • Miller J, McLachlan AD, Klug A. Repetitive zinc-binding domains in the protein transcription factor IIIA from Xenopus oocytes. EMBO J. 1985 Jun;4(6):1609–1614. [PMC free article] [PubMed]
  • Eckert RL, Green H. Structure and evolution of the human involucrin gene. Cell. 1986 Aug 15;46(4):583–589. [PubMed]
  • Teumer J, Green H. Divergent evolution of part of the involucrin gene in the hominoids: unique intragenic duplications in the gorilla and human. Proc Natl Acad Sci U S A. 1989 Feb;86(4):1283–1286. [PMC free article] [PubMed]
  • Ohno S. Birth of a unique enzyme from an alternative reading frame of the preexisted, internally repetitious coding sequence. Proc Natl Acad Sci U S A. 1984 Apr;81(8):2421–2425. [PMC free article] [PubMed]
  • Royle NJ, Clarkson RE, Wong Z, Jeffreys AJ. Clustering of hypervariable minisatellites in the proterminal regions of human autosomes. Genomics. 1988 Nov;3(4):352–360. [PubMed]
  • Armour JA, Wong Z, Wilson V, Royle NJ, Jeffreys AJ. Sequences flanking the repeat arrays of human minisatellites: association with tandem and dispersed repeat elements. Nucleic Acids Res. 1989 Jul 11;17(13):4925–4935. [PMC free article] [PubMed]
  • Vassart G, Georges M, Monsieur R, Brocas H, Lequarre AS, Christophe D. A sequence in M13 phage detects hypervariable minisatellites in human and animal DNA. Science. 1987 Feb 6;235(4789):683–684. [PubMed]
  • Dover G. Ignorant DNA? Nature. 1980 Jun 26;285(5767):618–620. [PubMed]

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