• We are sorry, but NCBI web applications do not support your browser and may not function properly. More information
Logo of narLink to Publisher's site
Nucleic Acids Res. Sep 25, 1989; 17(18): 7283–7294.
PMCID: PMC334808

Similarity of minus origins of replication and flanking open reading frames of plasmids pUB110, pTB913 and pMV158.


Plasmids pMV158 and pTB913, originating from Streptococcus agalactiae and a thermophilic Bacillus respectively, were sequenced to completion. Both contained a BA3-type minus origin of replication and an RSA-site, believed to constitute a site-specific recombination site. These two regions were more than 99% homologous to the corresponding regions of the Staphylococcus aureus plasmid pUB110. Deleting the BA3-type minus origin resulted in the accumulation of a considerable amount of single-stranded DNA, both in L. lactis subsp. lactis and B. subtilis, indicating that this minus origin was functional in both bacterial species. Like pUB110, both plasmids contained an open reading frame encoding a putative plasmid recombination enzyme (Pre protein), which was located downstream of the RSA-site. On the basis of sequence comparisons between pUB110, pMV158, pTB913, pT181, pE194, pNE131 and pT48 two distinct families of RSA-sites and Pre proteins could be distinguished.

Full text

Full text is available as a scanned copy of the original print version. Get a printable copy (PDF file) of the complete article (1.3M), or click on a page image below to browse page by page. Links to PubMed are also available for Selected References.

Images in this article

Click on the image to see a larger version.

Selected References

These references are in PubMed. This may not be the complete list of references from this article.
  • Burdett V. Identification of tetracycline-resistant R-plasmids in Streptococcus agalactiae (group B). Antimicrob Agents Chemother. 1980 Nov;18(5):753–760. [PMC free article] [PubMed]
  • Lacks SA, Lopez P, Greenberg B, Espinosa M. Identification and analysis of genes for tetracycline resistance and replication functions in the broad-host-range plasmid pLS1. J Mol Biol. 1986 Dec 20;192(4):753–765. [PubMed]
  • Puyet A, del Solar GH, Espinosa M. Identification of the origin and direction of replication of the broad-host-range plasmid pLS1. Nucleic Acids Res. 1988 Jan 11;16(1):115–133. [PMC free article] [PubMed]
  • del Solar GH, Puyet A, Espinosa M. Initiation signals for the conversion of single stranded to double stranded DNA forms in the streptococcal plasmid pLS1. Nucleic Acids Res. 1987 Jul 24;15(14):5561–5580. [PMC free article] [PubMed]
  • te Riele H, Michel B, Ehrlich SD. Single-stranded plasmid DNA in Bacillus subtilis and Staphylococcus aureus. Proc Natl Acad Sci U S A. 1986 Apr;83(8):2541–2545. [PMC free article] [PubMed]
  • te Riele H, Michel B, Ehrlich SD. Are single-stranded circles intermediates in plasmid DNA replication? EMBO J. 1986 Mar;5(3):631–637. [PMC free article] [PubMed]
  • van Wezenbeek PM, Hulsebos TJ, Schoenmakers JG. Nucleotide sequence of the filamentous bacteriophage M13 DNA genome: comparison with phage fd. Gene. 1980 Oct;11(1-2):129–148. [PubMed]
  • Gruss A, Ehrlich SD. The family of highly interrelated single-stranded deoxyribonucleic acid plasmids. Microbiol Rev. 1989 Jun;53(2):231–241. [PMC free article] [PubMed]
  • Horinouchi S, Weisblum B. Nucleotide sequence and functional map of pC194, a plasmid that specifies inducible chloramphenicol resistance. J Bacteriol. 1982 May;150(2):815–825. [PMC free article] [PubMed]
  • Horinouchi S, Weisblum B. Nucleotide sequence and functional map of pE194, a plasmid that specifies inducible resistance to macrolide, lincosamide, and streptogramin type B antibodies. J Bacteriol. 1982 May;150(2):804–814. [PMC free article] [PubMed]
  • Khan SA, Novick RP. Complete nucleotide sequence of pT181, a tetracycline-resistance plasmid from Staphylococcus aureus. Plasmid. 1983 Nov;10(3):251–259. [PubMed]
  • Bron S, Luxen E, Swart P. Instability of recombinant pUB110 plasmids in Bacillus subtilis: plasmid-encoded stability function and effects of DNA inserts. Plasmid. 1988 May;19(3):231–241. [PubMed]
  • Viret JF, Alonso JC. Generation of linear multigenome-length plasmid molecules in Bacillus subtilis. Nucleic Acids Res. 1987 Aug 25;15(16):6349–6367. [PMC free article] [PubMed]
  • Bron S, Bosma P, van Belkum M, Luxen E. Stability function in the Bacillus subtilis plasmid pTA 1060. Plasmid. 1987 Jul;18(1):8–15. [PubMed]
  • Chang S, Chang SY, Gray O. Structural and genetic analyses of a par locus that regulates plasmid partition in Bacillus subtilis. J Bacteriol. 1987 Sep;169(9):3952–3962. [PMC free article] [PubMed]
  • Devine KM, Hogan ST, Higgins DG, McConnell DJ. Replication and segregational stability of Bacillus plasmid pBAA1. J Bacteriol. 1989 Feb;171(2):1166–1172. [PMC free article] [PubMed]
  • Gryczan TJ, Dubnau D. Construction and properties of chimeric plasmids in Bacillus subtilis. Proc Natl Acad Sci U S A. 1978 Mar;75(3):1428–1432. [PMC free article] [PubMed]
  • McKenzie T, Hoshino T, Tanaka T, Sueoka N. The nucleotide sequence of pUB110: some salient features in relation to replication and its regulation. Plasmid. 1986 Mar;15(2):93–103. [PubMed]
  • Imanaka T, Fujii M, Aiba S. Isolation and characterization of antibiotic resistance plasmids from thermophilic bacilli and construction of deletion plasmids. J Bacteriol. 1981 Jun;146(3):1091–1097. [PMC free article] [PubMed]
  • Imanaka T, Ano T, Fujii M, Aiba S. Two replication determinants of an antibiotic-resistance plasmid, pTB19, from a thermophilic bacillus. J Gen Microbiol. 1984 Jun;130(6):1399–1408. [PubMed]
  • Matsumura M, Katakura Y, Imanaka T, Aiba S. Enzymatic and nucleotide sequence studies of a kanamycin-inactivating enzyme encoded by a plasmid from thermophilic bacilli in comparison with that encoded by plasmid pUB110. J Bacteriol. 1984 Oct;160(1):413–420. [PMC free article] [PubMed]
  • Muller RE, Ano T, Imanaka T, Aiba S. Complete nucleotide sequences of Bacillus plasmids pUB110dB, pRBH1 and its copy mutants. Mol Gen Genet. 1986 Jan;202(1):169–171. [PubMed]
  • Gennaro ML, Kornblum J, Novick RP. A site-specific recombination function in Staphylococcus aureus plasmids. J Bacteriol. 1987 Jun;169(6):2601–2610. [PMC free article] [PubMed]
  • Bron S, Venema G. Ultraviolet inactivation and excision-repair in Bacillus subtilis. I. Construction and characterization of a transformable eightfold auxotrophic strain and two ultraviolet-sensitive derivatives. Mutat Res. 1972 May;15(1):1–10. [PubMed]
  • Rottländer E, Trautner TA. Genetic and transfection studies with B, subtilis phage SP 50. I. Phage mutants with restricted growth on B. subtilis strain 168. Mol Gen Genet. 1970;108(1):47–60. [PubMed]
  • Terzaghi BE, Sandine WE. Improved medium for lactic streptococci and their bacteriophages. Appl Microbiol. 1975 Jun;29(6):807–813. [PMC free article] [PubMed]
  • Ish-Horowicz D, Burke JF. Rapid and efficient cosmid cloning. Nucleic Acids Res. 1981 Jul 10;9(13):2989–2998. [PMC free article] [PubMed]
  • van der Lelie D, Venema G. Bacillus subtilis generates a major specific deletion in pAM beta 1. Appl Environ Microbiol. 1987 Oct;53(10):2458–2463. [PMC free article] [PubMed]
  • Tautz D, Renz M. An optimized freeze-squeeze method for the recovery of DNA fragments from agarose gels. Anal Biochem. 1983 Jul 1;132(1):14–19. [PubMed]
  • Sanger F, Nicklen S, Coulson AR. DNA sequencing with chain-terminating inhibitors. Proc Natl Acad Sci U S A. 1977 Dec;74(12):5463–5467. [PMC free article] [PubMed]
  • van der Lelie D, van der Vossen JM, Venema G. Effect of Plasmid Incompatibility on DNA Transfer to Streptococcus cremoris. Appl Environ Microbiol. 1988 Apr;54(4):865–871. [PMC free article] [PubMed]
  • Kondo JK, McKay LL. Plasmid transformation of Streptococcus lactis protoplasts: optimization and use in molecular cloning. Appl Environ Microbiol. 1984 Aug;48(2):252–259. [PMC free article] [PubMed]
  • Mandel M, Higa A. Calcium-dependent bacteriophage DNA infection. J Mol Biol. 1970 Oct 14;53(1):159–162. [PubMed]
  • Chomczynski P, Qasba PK. Alkaline transfer of DNA to plastic membrane. Biochem Biophys Res Commun. 1984 Jul 18;122(1):340–344. [PubMed]
  • Novick RP, Iordanescu S, Surdeanu M, Edelman I. Transduction-related cointegrate formation between Staphylococcal plasmids: a new type of site-specific recombination. Plasmid. 1981 Sep;6(2):159–172. [PubMed]
  • Stassi DL, Lopez P, Espinosa M, Lacks SA. Cloning of chromosomal genes in Streptococcus pneumoniae. Proc Natl Acad Sci U S A. 1981 Nov;78(11):7028–7032. [PMC free article] [PubMed]
  • Lampson BC, Parisi JT. Nucleotide sequence of the constitutive macrolide-lincosamide-streptogramin B resistance plasmid pNE131 from Staphylococcus epidermidis and homologies with Staphylococcus aureus plasmids pE194 and pSN2. J Bacteriol. 1986 Sep;167(3):888–892. [PMC free article] [PubMed]
  • Catchpole I, Thomas C, Davies A, Dyke KG. The nucleotide sequence of Staphylococcus aureus plasmid pT48 conferring inducible macrolide-lincosamide-streptogramin B resistance and comparison with similar plasmids expressing constitutive resistance. J Gen Microbiol. 1988 Mar;134(3):697–709. [PubMed]
  • Pabo CO, Sauer RT. Protein-DNA recognition. Annu Rev Biochem. 1984;53:293–321. [PubMed]
  • Gruss AD, Ross HF, Novick RP. Functional analysis of a palindromic sequence required for normal replication of several staphylococcal plasmids. Proc Natl Acad Sci U S A. 1987 Apr;84(8):2165–2169. [PMC free article] [PubMed]
  • Chopin A, Chopin MC, Moillo-Batt A, Langella P. Two plasmid-determined restriction and modification systems in Streptococcus lactis. Plasmid. 1984 May;11(3):260–263. [PubMed]

Articles from Nucleic Acids Research are provided here courtesy of Oxford University Press


Related citations in PubMed

See reviews...See all...

Cited by other articles in PMC

See all...


Recent Activity

Your browsing activity is empty.

Activity recording is turned off.

Turn recording back on

See more...