• We are sorry, but NCBI web applications do not support your browser and may not function properly. More information
Logo of nihpaAbout Author manuscriptsSubmit a manuscriptNIH Public Access; Author Manuscript; Accepted for publication in peer reviewed journal;
Science. Author manuscript; available in PMC Apr 27, 2012.
Published in final edited form as:
PMCID: PMC3338249
NIHMSID: NIHMS355177

Clonogenic neoblasts are pluripotent adult stem cells that underlie planarian regeneration

Abstract

Pluripotent cells in the embryo can generate all cell types, but lineage-restricted cells are generally thought to replenish adult tissues. Planarians are flatworms and regenerate from tiny body fragments, a process requiring a population of proliferating cells (neoblasts). Whether regeneration is accomplished by pluripotent cells or by the collective activity of multiple lineage-restricted cell types is unknown. Using ionizing radiation and single-cell transplantation, we identified neoblasts that can form large descendant-cell colonies in vivo. These clonogenic neoblasts (cNeoblasts) produce cells that differentiate into neuronal, intestinal, and other known post-mitotic cell types and are distributed throughout the body. Single transplanted cNeoblasts restored regeneration in lethally irradiated hosts. We conclude that broadly distributed, adult pluripotent stem cells underlie the remarkable regenerative abilities of planarians.

Pluripotent cells, such as embryonic blastomeres, differentiate into mature cell types spanning three germ layers (13). Although essential for development, pluripotent cells are generally not known to be present in adult animals (4, 5). Adult tissues, by contrast, are typically maintained by specialized, tissue-specific adult stem cells (511). Planarians are flatworms well known for the ability to regenerate whole animals from small pieces of tissue (12). Planarian regeneration requires a population of proliferative cells, historically known as neoblasts, that exist throughout the body and collectively produce all known differentiated cell types (13, 14). Neoblasts have great potential for molecular genetic studies in Schmidtea mediterranea where a sequenced genome and molecular tools (including RNAi technology) enable identification and study of genes controlling regeneration (14, 15). To date, however, neoblast properties have only been studied at the level of a population (14, 1621). The cell population known as neoblasts, therefore, could either contain only lineage-restricted cells that together allow regeneration, or could contain, within the population, stem cells that are pluripotent at the single-cell level. A fundamental issue to address for understanding planarian regeneration, therefore, is the in vivo potential of individual proliferating planarian cells.

Colonies are generated from single smedwi-1+ cells following irradiation

We developed an in vivo method, utilizing ionizing radiation, that permits study of rare, individual proliferating cells and their descendants. Irradiation eliminates dividing cells and is a classic strategy for studying stem cells (22, 23). All dividing cells in adult planarians express the smedwi-1 gene (24) (Fig. 1A); these cells are specifically, rapidly, and completely depleted following exposure to high irradiation doses (e.g., 6,000 rads) (17, 24, 25). Low irradiation doses (i.e., 500 rads) eliminate some proliferating cells, leaving a large number spread ventrally throughout the animal (19). We identified an irradiation dose (1,750 rads) that eliminated all smedwi-1+ cells from most (78%) animals (Fig. 1B). However, seven days after 1,750 rad exposure, rare smedwi-1+ cells were present in the minority of animals (22%) as sparse “clusters” (Fig. 1B–C). Clusters consistently displayed compact, isolated, colony-like morphology and originated ventrally throughout the body (Fig. 1D, S1A–C) but were not associated with specific known tissues (Fig. S1D–E). These clusters, if resulting from clonal growth of single smedwi-1+ cells, provide the opportunity to study the developmental potential of individual planarian cells.

Figure 1
Expanding colonies are generated from isolated smedwi-1+ cells following irradiation

Numerous smedwi-1+ cluster attributes indicate they result from clonal growth. smedwi-1+ clusters were preceded by isolated smedwi-1+ cells present 3–4 days following irradiation, and typically displayed 3–10 cells after one week (Fig. 1E–F). Based on the low proportion of animals with smedwi-1+ cells in close proximity 3 days post-irradiation with 1,750 rads (Fig. S2), it is improbable that clusters arose from multiple adjacent smedwi-1+ cells (P = 0.0138, 2-tailed Fisher’s Exact Test). Cluster size increased dramatically over time, suggesting exponential growth, ultimately yielding hundreds of smedwi-1+ cells 14–18 days post-irradiation (Fig. 1E–F). Consistent with clusters originating from pre-existing smedwi-1+ cells that survived irradiation, a cluster location scatterplot resembled the normal smedwi-1+-expression pattern (Fig. 1D), and cluster frequency decreased with increasing irradiation doses (see below, Fig. 3A). BrdU delivery labels smedwi-1-expressing cells (ref (24) and Fig. S3A), followed by a rapid decline in incorporation within 24–48 hours (ref (17) and Fig. S3B–D) demonstrating unincorporated BrdU does not perdure long term. A BrdU pulse followed by irradiation resulted in clusters consisting entirely of BrdU+; smedwi-1+ cells (Fig. S3E), indicating smedwi-1+-cluster expansion results from division of existing smedwi-1+ cells (i.e., by clonal growth). If some other process, such as dedifferentiation, produced smedwi-1+ cluster cells, these cells should have been BrdU.

Following irradiation, every proliferative cell detected by an 8-hour BrdU pulse (Fig. 1G), or by using probes for the conserved proliferation marker genes histone h2b (Smed-h2b) (26), pcna (Smed-pcna) (27), or ribonucleotide reductase (Smed-RRM2-1) (17, 28) (Fig. S3F–H), existed in clusters and expressed smedwi-1 (n=815). Therefore, no other source (non-smedwi-1+) for proliferating cells exists outside of smedwi-1+ clusters in irradiated animals. Furthermore, if additional sources for smedwi-1+ cells (other than clonal growth) existed, cluster number would be expected to increase with time and small, newly formed clusters might be present at late timepoints following irradiation. Neither of these possibilities was observed (Fig. 1F). New cluster production was also not observed following amputation or feeding (Fig. S4), which elicit proliferative responses (29, 30). These data indicate that clonal expansion (producing colonies) represents the source of new smedwi-1+ dividing cells during cluster formation and growth.

Not all proliferating cells (neoblasts) necessarily have the capacity to form colonies. We term cells displaying this capacity clonogenic neoblasts (cNeoblasts); these cells express smedwi-1, have a body-wide (head-to-tail) distribution (Fig. 1D), and generate large, expanding colonies of smedwi-1+ cells. The ability of small numbers of colonies ultimately to restore both smedwi-1+ cells and mitotic activity to normal levels (Fig. S5) suggests a stem cell-like capacity for self-renewal.

To investigate the potential of individual cNeoblasts, we analyzed smedwi-1+ colonies using three well-established differentiation assays involving a SMEDWI-1 antibody (24, 29, 31), BrdU pulse-chase (17, 18, 31), and post-mitotic cell type markers (17, 29, 31, 32). SMEDWI-1 protein is present in smedwi-1 mRNA+ dividing cells and temporarily remains detectable in post-mitotic descendant cells (24). Differentiating cells therefore transit through a SMEDWI-1(protein)+;smedwi-1(mRNA) state (29, 31). All colonies examined (12/12) contained SMEDWI-1+; smedwi-1 cells (Fig. 1H). Independently, BrdU can label cells that divide and exit the smedwi-1+ state. All colonies analyzed by 4-day BrdU pulse-chase (31/31) contained BrdU+; smedwi-1 cells (Fig. 1I) and no BrdU+ cells existed in worms lacking smedwi-1+ colonies (25/25 animals), indicating that colonies produce, and are the only source for, cells exiting the smedwi-1+ undifferentiated state. SMEDWI-1+ or BrdU+ colony cells can thus be assessed for lineage-specific marker expression to determine the developmental potential of individual cNeoblasts.

cNeoblasts display broad differentiation capacity

Described adult stem cells typically only produce differentiated cells corresponding to their germ layer and tissue of origin (5). To address whether cNeoblasts, by contrast, could produce cell types derived from multiple germ layers, we identified and characterized markers for neuronal (ectoderm-derived) and intestinal (endoderm-derived) lineages. In untreated animals, some SMEDWI-1+ descendant cells expressed a choline acetyl-transferase ortholog, Smed-chat (Fig. S6); chat expression is widely conserved in cholinergic neurons (33). SMEDWI-1+; chat+ cells were enriched in brain regions, had neuronal morphology, and chat+ cells co-expressed additional neuronal markers (Fig. S6), indicating that SMEDWI-1+; chat+ cells are differentiating neurons. Smed-gata4/5/6 and Smed-hnf4, orthologs of endoderm-promoting GATA4/5/6 and HNF4 transcription factors, respectively (34), were expressed in intestinal cells and also in interspersed cells surrounding the intestine (Fig. S7–10). Many of these interspersed cells were irradiation-sensitive and SMEDWI-1+, indicating they represent differentiating endodermal cells (Fig. S8, S10). A third endoderm marker gene used, Smed-mat, was expressed in intestinal branches (Fig. S8, S10). Finally, additional differentiation marker genes used (Smed-AGAT-1, NB.21.11E, Smed-MCP-1, Smed-ODC-1, Smed-CYP1A1-1, and NB.52.12F) are expressed in partially overlapping mesenchymal populations of post-mitotic cells (Fig. S11A–F) (17). These populations have unknown lineage relationships but turnover rapidly, and are consequently depleted following irradiation (17) (Fig. S11G–L).

Using SMEDWI-1 to label colony cell descendants, individual colonies were examined for the presence of both gata4/5/6+ and chat+, both gata4/5/6+ and AGAT-1+, or both AGAT-1+ and chat+ differentiating cells. In nearly all cases (n=20/22), individual colonies contained newly produced cells of both lineages tested (Fig. 2A–C, S12). The 1,750 rad dose yields rare, well-separated colonies (See Fig. 1C); animals fixed 7 days after irradiation contained single colonies (12/28 animals), no colonies (12/28), and only rarely, more than one colony (4/28). Given the high frequency of colonies producing multiple lineages (20/22), it is improbable that all such cases were the result of multiple colonies merging (P<0.0001, Fisher’s Exact Test). In addition, using a 4-day BrdU pulse chase as an independent method, we identified several colonies containing both BrdU+; chat+ (neuronal) and BrdU+; mat+ (intestinal) cells (Fig. 2D). Nearly all colonies examined, using the SMEDWI-1 antibody or BrdU, produced differentiated cells for any single lineage analyzed (n=61/64) (Fig. S13). These colonies were distributed throughout the body and not restricted to specific anatomical regions (Fig. 2E). Finally, nearly every smedwi-1+ colony examined had associated cells expressing every additional differentiation marker tested (NB.21.11E, MCP-1, ODC-1, CYP1A1-1, NB.52.12F) (n=110/115, Fig. S14A–E); descendant cell clusters, furthermore, were never observed in regions lacking smedwi-1+ colonies (Fig. S11G–L). In addition, even early colonies (7 days post-irradiation) had associated differentiated cells (Fig. S14F). Together, these data indicate that broad multipotency and a body-wide distribution are fundamental attributes of individual cNeoblasts.

Figure 2
Clonogenic neoblasts display broad differentiation capacity

Small numbers of cNeoblasts restore regenerative ability

Irradiated planarians cannot regenerate (35) and suffer massive tissue loss because of failed replacement of aged differentiated cells (35, 36). However, transplantation of large numbers of cells (16) or tissue fragments can restore regenerative ability to irradiated hosts and change sexual behavior to that of a donor (16, 20, 21, 37). We sought to determine whether small numbers of cNeoblasts would restore regenerative ability to irradiated animals. Following irradiation, some animals were fixed and colony numbers determined; remaining animals were followed to assess survival and regeneration frequencies. Increasing irradiation doses resulted in decreasing colony numbers (Fig. 3A–B) and survival rates (Fig. S15A). Regeneration, which involves production of diverse cell types (Fig. S16), was initially impeded in animals cut 4 days post-irradiation (Fig. S15B–C); however, many animals both survived and ultimately regenerated at doses that produced sparse, measurable colony numbers (Fig. 3A–D, Table S1). These animals regenerated heads containing neurons (ectoderm), muscle (mesoderm), and intestine (endoderm) (Fig. 3D–E). The minimum number of cNeoblasts initially present in irradiation survivors can be estimated by comparing the number of colonies present (in fixed animals) to observed regeneration frequencies (see Table S1). Our data indicate as few as three (P=0.0478), four (P=0.0017), or five colonies (P<0.0001, Fisher’s Exact Test) can be sufficient to restore regenerative ability to entire animals.

Figure 3
Small numbers of cNeoblasts can restore tissue turnover and regenerative ability

Transplantation of individual cNeoblasts

To determine whether a single cNeoblast can generate all essential adult cells, we developed a method for isolating and transplanting individual cNeoblasts into lethally irradiated hosts. Previous flow cytometry studies identified an irradiation-sensitive cell population with a high percentage of smedwi-1+ cells (25, 38). However, the Hoechst 33342 DNA dye used in this method is cytotoxic. Therefore, size and complexity properties of cells within the X1 fraction were used to define a gate for sorting unlabeled cells, which we refer to as the X1(FS) fraction (Fig. 4A–B).

Figure 4
Single transplanted cNeoblasts display properties of clonal growth and multipotency

X1(FS) cells are heterogeneous; however, cells with a similar morphology to X1 cells can be identified microscopically (Fig. 4C). Single selected cells were loaded into needles and transplanted post-pharyngeally into lethally irradiated hosts (Fig. 4D). To confirm that only single cells were transplanted, needles were loaded and the contents expelled into media. In all cases, only a single cell was observed exiting the needle (n=136/136). Furthermore, some animals were fixed immediately following transplant and labeled with a smedwi-1 RNA probe. All injected animals had either one (n=20/60) or zero (n=40/60) smedwi-1+ cells (Fig. 4E–F).

If a transplanted cell was a cNeoblast capable of engraftment, then clonal growth of progeny cells would be expected. Indeed, animals examined 6 days after single cell transplantation displayed clusters with 1 to 13 smedwi-1+ cells (n=23/100) (Fig. 4G). Furthermore, selecting for X1(FS) cells that were approximately 10µm in diameter and that had blebs and/or cytoplasmic processes increased engraftment rates, ranging from 12% (n=17) to 75% (n=20) (Fig. S17). Cells with properties of cNeoblasts, therefore, are present in the X1(FS) fraction and can be successfully transplanted.

If cNeoblasts are pluripotent stem cells capable of self-renewal, then a single cNeoblast should, in principle, be capable of restoring tissue turnover and regenerative capacity to lethally irradiated hosts. However, for this to occur the irradiated host must survive long enough for the cNeoblast to repopulate the smedwi-1+ population and replenish dying tissue. We therefore used a sexual S. mediterranea strain (S2F1L3F2) that can survive longer than the asexual strain (CIW4) after a 6,000 rad irradiation dose (Fig. S18). Sexual hosts transplanted with single asexual cells had colonies consisting of large numbers of SMEDWI-1+ cells 30 days after transplantation (n=4/17) (Fig. 4H). Every colony examined contained SMEDWI-1+; Smed-gata4/5/6+ double-positive cells (n=4/4) and most of these colonies also contained SMEDWI-1+; Smed-chat+ double-positive cells (n=3/4) (Fig. 4H). Transplant data thus independently confirm attributes of colonies described post 1,750 rads, further indicating that clonal growth and multipotency are important features of individual cNeoblasts.

Entire animals and strains regenerated from a single transplanted cNeoblast

Two weeks after irradiation, lesions appeared at sexual animal head tips, followed by progressive anterior to posterior tissue regression with 100% penetrant animal death after approximately six weeks (n=78) (Fig. 5A). Remarkably, several transplant recipients lived past seven weeks and eventually developed blastemas at the site of tissue regression (n=7/130) (Fig. 5A). Animals that developed blastemas regenerated anterior and midbody structures, such as photoreceptors and pharynges (Fig. 5A), and regained feeding behavior by eight weeks after irradiation. Of the seven rescued animals, three were expanded into strains (R1, R2, and R3) by serial amputation and regeneration (Fig. S19). These animals exhibited normal blastema formation and the capacity to regenerate photoreceptors and intestine following amputation (Fig. 5B). The ability to produce multiple regenerating animals from a single transplanted cell indicates a self-renewing capability of cNeoblasts. Rescued strains did not display sexual features, such as large size and a gonopore; by contrast, animals in all three strains reproduced by binary fission, an asexual behavior seen very rarely in sexual animals (Fig. S19).

Figure 5
Restoration of regeneration in lethally irradiated hosts by single transplanted cNeoblasts

To confirm that all new tissue in rescued strains resulted from clonal division from the donor cNeoblast, we genotyped the animals using SNPs identified between the asexual strain and the sexual strain (see Materials and Methods). Genomic DNA was isolated from strain R1, R2, and R3 animals following two rounds of regeneration; growth and regeneration should replace host tissues with donor-derived cells (Fig. 6A). If, on the other hand, host cells continue to replenish tissues after irradiation, host SNPs in the collected genomic DNA would be expected. PCR-RFLP analysis of two loci (RFLP 00310 and RFLP 00463) revealed that the rescued strains have the asexual strain RFLPs, indicating that the majority of cells in these animals were donor-derived (Fig. 6B). Sequencing of three independent homozygous haplotypes (00163, 00463, and 02716), each containing six SNPs that distinguish asexual CIW4 and sexual S2F1L3F2 strains, confirmed that the rescue strains possessed the donor, rather than host, genotype (Fig. 6C). These data indicate that descendants of a single cNeoblast ultimately transformed the recipient into a genetic clone of the donor by replacing all cells present in the original host. We conclude that cNeoblasts are pluripotent stem cells with a broad, body-wide distribution and that persistence into adulthood of a pluripotent stem cell enables the remarkable regenerative feats of planarians.

Figure 6
Genotype conversion by single transplanted cNeoblasts

Supplementary Material

Supplemental Online Material

Acknowledgments

We thank D. Kim for manuscript comments, S. Lapan for intestinal markers, M. Srivastava for phylogenetics advice, D. Wenemoser for SMEDWI-1 antibody purification, J. Owen for Illumina data, M. Griffin for flow cytometry assistance, as well as P. Hsu, G. Bell, R. Young, and all members of the Reddien Lab for extensive comments and discussion. P.W.R. is an early career scientist of the Howard Hughes Medical Institute. We acknowledge support by NIH R01GM080639 and Keck Foundation support.

References

1. Evans MJ. J Embryol Exp Morphol. 1972;28:163. [PubMed]
2. Evans MJ, Kaufman MH. Nature. 1981;292:154. [PubMed]
3. Martin GR. Proc Natl Acad Sci USA. 1981;78:7634. [PMC free article] [PubMed]
4. Wagers AJ, Sherwood RI, Christensen JL, Weissman IL. Science. 2002;297:2256. [PubMed]
5. Wagers AJ, Weissman IL. Cell. 2004;116:639. [PubMed]
6. Weissman IL. Cell. 2000;100:157. [PubMed]
7. Spangrude GJ, Heimfeld S, Weissman IL. Science. 1988;241:58. [PubMed]
8. Uchida N, et al. Proc Natl Acad Sci USA. 2000;97:14720. [PMC free article] [PubMed]
9. Blanpain C, Lowry WE, Geoghegan A, Polak L, Fuchs E. Cell. 2004;118:635. [PubMed]
10. Ohlstein B, Spradling A. Nature. 2006;439:470. [PubMed]
11. Barker N, et al. Nature. 2007;449:1003. [PubMed]
12. Morgan TH. Arch. Entw. Mech. Org. 1898;7:364.
13. Keller J. Jen Zeit Naturw. 1894;28:370.
14. Reddien PW, Sánchez Alvarado A. Annu Rev Cell Dev Biol. 2004;20:725. [PubMed]
15. Reddien PW, Bermange AL, Murfitt KJ, Jennings JR, Sánchez Alvarado A. Dev Cell. 2005;8:635. [PMC free article] [PubMed]
16. Baguñà J, Saló E, Auladell C. Development. 1989;107:77.
17. Eisenhoffer GT, Kang H, Sánchez Alvarado A. Cell Stem Cell. 2008;3:327. [PMC free article] [PubMed]
18. Newmark PA, Sánchez Alvarado A. Dev Biol. 2000;220:142. [PubMed]
19. Salvetti A, et al. Dev Biol. 2009;328:305. [PubMed]
20. Lange CS, Gilbert CW. International Journal of Radiation Biology. 1968;14:373. [PubMed]
21. Wolff E, Dubois F. Revue Swisse Zool. 1948;55:218.
22. Becker AJ, Till JE, McCulloch EA. Nature. 1963;197:452. [PubMed]
23. Till JE, McCulloch E. Radiat Res. 1961;14:213. [PubMed]
24. Guo T, Peters AH, Newmark PA. Dev Cell. 2006;11:159. [PubMed]
25. Reddien PW, Oviedo NJ, Jennings JR, Jenkin JC, Sánchez Alvarado A. Science. 2005;310:1327. [PubMed]
26. Hewitson TD, Kelynack KJ, Darby IA. Methods Mol Biol. 2006;326:219. [PubMed]
27. Bravo R, Frank R, Blundell PA, Macdonald-Bravo H. Nature. 1987;326:515. [PubMed]
28. Eriksson S, Graslund A, Skog S, Thelander L, Tribukait B. J Biol Chem. 1984;259:11695. [PubMed]
29. Wenemoser D, Reddien PW. Dev Biol. 2010;344:979. [PMC free article] [PubMed]
30. Baguñà J. J. Exp. Zool. 1976;195:53.
31. Scimone ML, Meisel J, Reddien PW. Development. 2010;137:1231. [PMC free article] [PubMed]
32. Pearson BJ, Sánchez Alvarado A. Development. 2010;137:213. [PMC free article] [PubMed]
33. Nishimura K, Kitamura Y, Taniguchi T, Agata K. Neuroscience. 2010;1:18. [PubMed]
34. Morrisey EE, et al. Genes Dev. 1998;12:3579. [PMC free article] [PubMed]
35. Bardeen CR, Baetjer FH. J. Exp. Zool. 1904;1:191.
36. Dubois F. Bull. Biol. Fr. Belg. 1949;83:213.
37. Lender T, Gabriel A. C. R, Acad. Sc. Paris. 1965;260:4095. [PubMed]
38. Hayashi T, Asami M, Higuchi S, Shibata N, Agata K. Dev Growth Differ. 2006;48:371. [PubMed]
PubReader format: click here to try

Formats:

Related citations in PubMed

See reviews...See all...

Cited by other articles in PMC

See all...

Links

Recent Activity

Your browsing activity is empty.

Activity recording is turned off.

Turn recording back on

See more...