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Nucleic Acids Res. Feb 25, 1991; 19(4): 933–937.
PMCID: PMC333734

An enhancer at the 3' end of the mouse immunoglobulin heavy chain locus.

Abstract

A tissue-specific enhancer (E mu) lies between the joining (JH) and mu constant region (C mu) gene segments of the immunoglobulin heavy chain (IgH) locus. Since mouse endogenous IgH genes are efficiently transcribed in its absence, the normal function of this enhancer remains ill-defined. Recently, another lymphoid-specific enhancer of equal strength has been identified 3' of the rat IgH locus. We have isolated an analogous sequence from mouse and have mapped it 12.5 kb 3' of the 3'-most constant region gene (C alpha-membrane) of the BALB/c mouse locus. The mouse and rat sequences are 82% homologous and share with other enhancers several DNA sequence motifs capable of binding protein. However, in transient transfection assays, the mouse sequence behaves as a weaker enhancer. The role of this distant element in the expression of endogenous IgH genes, both in E mu-deficient, Ig-producing cell lines and during normal B cell development, is discussed.

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Selected References

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  • Eckhardt LA, Tilley SA, Lang RB, Marcu KB, Birshtein BK. DNA rearrangements in MPC-11 immunoglobulin heavy chain class-switch variants. Proc Natl Acad Sci U S A. 1982 May;79(9):3006–3010. [PMC free article] [PubMed]
  • Stavnezer J. Presence of a polyadenylated RNA fragment encoding the membrane domain for immunoglobulin alpha chain indicates that mRNAs for both secreted and membrane-bound alpha chains can be produced from the same RNA transcript. Nucleic Acids Res. 1986 Aug 11;14(15):6129–6144. [PMC free article] [PubMed]
  • Grosschedl R, Baltimore D. Cell-type specificity of immunoglobulin gene expression is regulated by at least three DNA sequence elements. Cell. 1985 Jul;41(3):885–897. [PubMed]
  • Han K, Levine MS, Manley JL. Synergistic activation and repression of transcription by Drosophila homeobox proteins. Cell. 1989 Feb 24;56(4):573–583. [PubMed]
  • Hozumi N, Wu GE, Murialdo H, Roberts L, Vetter D, Fife WL, Whiteley M, Sadowski P. RNA splicing mutation in an aberrantly rearranged immunoglobulin lambda I gene. Proc Natl Acad Sci U S A. 1981 Nov;78(11):7019–7023. [PMC free article] [PubMed]
  • Bernard O, Hozumi N, Tonegawa S. Sequences of mouse immunoglobulin light chain genes before and after somatic changes. Cell. 1978 Dec;15(4):1133–1144. [PubMed]
  • Peterson DO, Beifuss KK, Morley KL. Context-dependent gene expression: cis-acting negative effects of specific procaryotic plasmid sequences on eucaryotic genes. Mol Cell Biol. 1987 Apr;7(4):1563–1567. [PMC free article] [PubMed]
  • Gorman CM, Moffat LF, Howard BH. Recombinant genomes which express chloramphenicol acetyltransferase in mammalian cells. Mol Cell Biol. 1982 Sep;2(9):1044–1051. [PMC free article] [PubMed]
  • Kadesch T, Zervos P, Ruezinsky D. Functional analysis of the murine IgH enhancer: evidence for negative control of cell-type specificity. Nucleic Acids Res. 1986 Oct 24;14(20):8209–8221. [PMC free article] [PubMed]
  • Kiledjian M, Su LK, Kadesch T. Identification and characterization of two functional domains within the murine heavy-chain enhancer. Mol Cell Biol. 1988 Jan;8(1):145–152. [PMC free article] [PubMed]
  • Jones NC, Rigby PW, Ziff EB. Trans-acting protein factors and the regulation of eukaryotic transcription: lessons from studies on DNA tumor viruses. Genes Dev. 1988 Mar;2(3):267–281. [PubMed]
  • Calame K, Eaton S. Transcriptional controlling elements in the immunoglobulin and T cell receptor loci. Adv Immunol. 1988;43:235–275. [PubMed]
  • Libermann TA, Lenardo M, Baltimore D. Involvement of a second lymphoid-specific enhancer element in the regulation of immunoglobulin heavy-chain gene expression. Mol Cell Biol. 1990 Jun;10(6):3155–3162. [PMC free article] [PubMed]
  • Nelsen B, Kadesch T, Sen R. Complex regulation of the immunoglobulin mu heavy-chain gene enhancer: microB, a new determinant of enhancer function. Mol Cell Biol. 1990 Jun;10(6):3145–3154. [PMC free article] [PubMed]
  • Picard D, Schaffner W. A lymphocyte-specific enhancer in the mouse immunoglobulin kappa gene. Nature. 1984 Jan 5;307(5946):80–82. [PubMed]
  • Klein S, Gerster T, Picard D, Radbruch A, Schaffner W. Evidence for transient requirement of the IgH enhancer. Nucleic Acids Res. 1985 Dec 20;13(24):8901–8912. [PMC free article] [PubMed]
  • Tilley SA, Birshtein BK. Unequal sister chromatid exchange. A mechanism affecting Ig gene arrangement and expression. J Exp Med. 1985 Aug 1;162(2):675–694. [PMC free article] [PubMed]
  • Cockerill PN. Nuclear matrix attachment occurs in several regions of the IgH locus. Nucleic Acids Res. 1990 May 11;18(9):2643–2648. [PMC free article] [PubMed]
  • Gillies SD, Morrison SL, Oi VT, Tonegawa S. A tissue-specific transcription enhancer element is located in the major intron of a rearranged immunoglobulin heavy chain gene. Cell. 1983 Jul;33(3):717–728. [PubMed]
  • Banerji J, Olson L, Schaffner W. A lymphocyte-specific cellular enhancer is located downstream of the joining region in immunoglobulin heavy chain genes. Cell. 1983 Jul;33(3):729–740. [PubMed]
  • Aguilera RJ, Hope TJ, Sakano H. Characterization of immunoglobulin enhancer deletions in murine plasmacytomas. EMBO J. 1985 Dec 30;4(13B):3689–3693. [PMC free article] [PubMed]
  • Klein S, Sablitzky F, Radbruch A. Deletion of the IgH enhancer does not reduce immunoglobulin heavy chain production of a hybridoma IgD class switch variant. EMBO J. 1984 Nov;3(11):2473–2476. [PMC free article] [PubMed]
  • Wabl MR, Burrows PD. Expression of immunoglobulin heavy chain at a high level in the absence of a proposed immunoglobulin enhancer element in cis. Proc Natl Acad Sci U S A. 1984 Apr;81(8):2452–2455. [PMC free article] [PubMed]
  • Eckhardt LA, Birshtein BK. Independent immunoglobulin class-switch events occurring in a single myeloma cell line. Mol Cell Biol. 1985 Apr;5(4):856–868. [PMC free article] [PubMed]
  • Zaller DM, Eckhardt LA. Deletion of a B-cell-specific enhancer affects transfected, but not endogenous, immunoglobulin heavy-chain gene expression. Proc Natl Acad Sci U S A. 1985 Aug;82(15):5088–5092. [PMC free article] [PubMed]
  • Koskimies S, Birshtein BK. Primary and secondary variants in immunoglobulin heavy chain production. Nature. 1976 Dec 2;264(5585):480–482. [PubMed]
  • Porton B, Zaller DM, Lieberson R, Eckhardt LA. Immunoglobulin heavy-chain enhancer is required to maintain transfected gamma 2A gene expression in a pre-B-cell line. Mol Cell Biol. 1990 Mar;10(3):1076–1083. [PMC free article] [PubMed]
  • Grosschedl R, Marx M. Stable propagation of the active transcriptional state of an immunoglobulin mu gene requires continuous enhancer function. Cell. 1988 Nov 18;55(4):645–654. [PubMed]
  • Gregor PD, Morrison SL. Myeloma mutant with a novel 3' flanking region: loss of normal sequence and insertion of repetitive elements leads to decreased transcription but normal processing of the alpha heavy-chain gene products. Mol Cell Biol. 1986 Jun;6(6):1903–1916. [PMC free article] [PubMed]
  • Kakkis E, Mercola M, Calame K. Strong transcriptional activation of translocated c-myc genes occurs without a strong nearby enhancer or promoter. Nucleic Acids Res. 1988 Jan 11;16(1):77–96. [PMC free article] [PubMed]
  • Pettersson S, Cook GP, Brüggemann M, Williams GT, Neuberger MS. A second B cell-specific enhancer 3' of the immunoglobulin heavy-chain locus. Nature. 1990 Mar 8;344(6262):165–168. [PubMed]
  • Saiki RK, Gelfand DH, Stoffel S, Scharf SJ, Higuchi R, Horn GT, Mullis KB, Erlich HA. Primer-directed enzymatic amplification of DNA with a thermostable DNA polymerase. Science. 1988 Jan 29;239(4839):487–491. [PubMed]
  • Messing J, Vieira J. A new pair of M13 vectors for selecting either DNA strand of double-digest restriction fragments. Gene. 1982 Oct;19(3):269–276. [PubMed]
  • Sanger F, Nicklen S, Coulson AR. DNA sequencing with chain-terminating inhibitors. Proc Natl Acad Sci U S A. 1977 Dec;74(12):5463–5467. [PMC free article] [PubMed]

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