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Logo of nihpaAbout Author manuscriptsSubmit a manuscriptNIH Public Access; Author Manuscript; Accepted for publication in peer reviewed journal;
Curr Biol. Author manuscript; available in PMC Apr 24, 2012.
Published in final edited form as:
PMCID: PMC3335243
NIHMSID: NIHMS139905

Reprogramming after chromosome transfer into mouse blastomeres

Summary

It is well known that oocytes can reprogram differentiated cells, allowing animal cloning by nuclear transfer. We have recently shown that fertilized zygotes retain reprogramming activities [], suggesting such activities might also persist in cleavage stage embryos. We have used chromosome transplantation techniques to investigate whether the blastomeres of two-cell stage mouse embryos can reprogram more differentiated cells. When chromosomes from one of the two blastomeres were replaced with the chromosomes of an embryonic or CD4+ T-lymphocyte donor cell, we observed nuclear reprogramming and efficient contribution of the manipulated cell to the developing blastocyst. Embryos produced by this method could be used to derive stem cell lines and also developed to term, generating mosaic “cloned” animals. These results demonstrate that blastomeres retain reprogramming activities and support the notion that discarded human preimplantation embryos may be useful recipients for the production of genetically tailored human embryonic stem cell lines.

Keywords: blastomere, mitosis, chromosome condensation, stem cell, reprogramming, development
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