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J Clin Invest. 1977 Jan; 59(1): 176–178.
PMCID: PMC333345

Antinuclear antibody with distinct specificity for polymyositis.


In the course of studying antinuclear antibodies in the rheumatic diseases, a new precipitin reaction (provisionally referred to as PM-1) was observed between calf thymus nuclear extract and polymyositis sera. Objectives of this study were to further define the immunologic nature of this reaction and to determine its specificity for polymyositis. Immunodiffusion studies using calf thymus nuclear extract revealed the PM-1 precipitin line in 17 of 28 patients with polymyositis. This reaction was not produced by sera of 460 patients with other diseases. Enzyme and heat treatments of the nuclear extract showed that PM-1 was distinct from native DNA, ribonucleoprotein, and Sm antigens. Fractionation of PM-1-positive serum by 30% ammonium sulphate and Sephadex G-200 chromatography revealed that the factor producing the PM-1 precipitin reaction was in a serum fraction which showed only IgG by immunoelectrphoresis against anti-whole human serum. Because of the apparent strong specificity, the PM-1 system may represent a marker antibody for polymyositis.

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Selected References

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  • Sharp GC, Irvin WS, LaRoque RL, Velez C, Daly V, Kaiser AD, Holman HR. Association of autoantibodies to different nuclear antigens with clinical patterns of rheumatic disease and responsiveness to therapy. J Clin Invest. 1971 Feb;50(2):350–359. [PMC free article] [PubMed]
  • Sharp GC, Irvin WS, Tan EM, Gould RG, Holman HR. Mixed connective tissue disease--an apparently distinct rheumatic disease syndrome associated with a specific antibody to an extractable nuclear antigen (ENA). Am J Med. 1972 Feb;52(2):148–159. [PubMed]
  • Stern GM, Rose AL, Jacobs K. Circulating antibodies in polymyositis. J Neurol Sci. 1967 Jul-Aug;5(1):181–183. [PubMed]
  • WEIR DM, HOLBOROW EJ, JOHNSON GD. A clinical study of serum antinuclear factor. Br Med J. 1961 Apr 1;1(5230):933–937. [PMC free article] [PubMed]
  • Tan EM, Pearson CM. Rheumatic disease sera reactive with capillaries in the mouse kidney. Arthritis Rheum. 1972 Jan-Feb;15(1):23–28. [PubMed]
  • Reichlin M, Mattioli M. Description of a serological reaction characteristic of polymyositis. Clin Immunol Immunopathol. 1976 Jan;5(1):12–20. [PubMed]

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