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Nucleic Acids Res. Oct 25, 1990; 18(20): 6025–6029.
PMCID: PMC332400

Thiophosphate interference experiments locate phosphates important for the hammerhead RNA self-cleavage reaction.

Abstract

A hammerhead domain of less than 50 nucleotides is responsible for a self-cleavage reaction in the replication of plant RNA pathogens. The hammerhead is composed of three helices joining at a central conserved core of 11 single stranded nucleotides. The core is believed to fold into a tertiary structure that provides functional groups for catalysis and to coordinate one or more divalent metal ions. In this study we use a phosphorothioate substitution interference assay to identify four phosphates in the conserved core which also play a role in the self-cleavage reaction.

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Selected References

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