• We are sorry, but NCBI web applications do not support your browser and may not function properly. More information
Logo of narLink to Publisher's site
Nucleic Acids Res. Apr 25, 1990; 18(8): 2033–2036.
PMCID: PMC330679

Cloning and analysis of three new homeobox genes from the nematode Caenorhabditis elegans.

Abstract

Three homeobox-containing genes from the nematode Caenorhabditis elegans are described. Two of them (ceh-11 and ceh-12) were isolated from a genomic library by hybridization at low stringency with the Ascaris lumbricoides homeobox AHB-1. The first clone contains a homeobox defining a new class of homeoboxes (ceh-11). This gene maps on the third chromosome of C. elegans, at the same locus as egl-5, a gene already known to be essential for the determination of specific neurons. In the second clone, sequence analysis revealed the existence of the third helix of a putative homeobox (ceh-12) which is interrupted by an intron located upstream of the codon for the amino acid 45 of the homeodomain. Using the ceh-11 homeobox as a probe, a third homeobox (ceh-13) was isolated from a cDNA library. As ceh-13 belongs to the labial class of homeoboxes, we conclude that, at the time when the nematode lineage diverged from the myriapod-insect and the vertebrate lineages, the duplication which led to the Antp and the labial families of homeoboxes had already taken place.

Full text

Full text is available as a scanned copy of the original print version. Get a printable copy (PDF file) of the complete article (696K), or click on a page image below to browse page by page. Links to PubMed are also available for Selected References.

Selected References

These references are in PubMed. This may not be the complete list of references from this article.
  • Gehring WJ. Homeo boxes in the study of development. Science. 1987 Jun 5;236(4806):1245–1252. [PubMed]
  • Scott MP, Tamkun JW, Hartzell GW., 3rd The structure and function of the homeodomain. Biochim Biophys Acta. 1989 Jul 28;989(1):25–48. [PubMed]
  • Way JC, Chalfie M. mec-3, a homeobox-containing gene that specifies differentiation of the touch receptor neurons in C. elegans. Cell. 1988 Jul 1;54(1):5–16. [PubMed]
  • Costa M, Weir M, Coulson A, Sulston J, Kenyon C. Posterior pattern formation in C. elegans involves position-specific expression of a gene containing a homeobox. Cell. 1988 Dec 2;55(5):747–756. [PubMed]
  • Finney M, Ruvkun G, Horvitz HR. The C. elegans cell lineage and differentiation gene unc-86 encodes a protein with a homeodomain and extended similarity to transcription factors. Cell. 1988 Dec 2;55(5):757–769. [PubMed]
  • Bürglin TR, Finney M, Coulson A, Ruvkun G. Caenorhabditis elegans has scores of homoeobox-containing genes. Nature. 1989 Sep 21;341(6239):239–243. [PubMed]
  • Otting G, Qian YQ, Müller M, Affolter M, Gehring W, Wüthrich K. Secondary structure determination for the Antennapedia homeodomain by nuclear magnetic resonance and evidence for a helix-turn-helix motif. EMBO J. 1988 Dec 20;7(13):4305–4309. [PMC free article] [PubMed]
  • Chen HZ, Hoey T, Zubay G. Purification and properties of the Drosophila zen protein. Mol Cell Biochem. 1988 Feb;79(2):181–189. [PubMed]
  • Desplan C, Theis J, O'Farrell PH. The Drosophila developmental gene, engrailed, encodes a sequence-specific DNA binding activity. Nature. 1985 Dec 19;318(6047):630–635. [PMC free article] [PubMed]
  • Hoey T, Levine M. Divergent homeo box proteins recognize similar DNA sequences in Drosophila. Nature. 1988 Apr 28;332(6167):858–861. [PubMed]
  • Fainsod A, Bogarad LD, Ruusala T, Lubin M, Crothers DM, Ruddle FH. The homeo domain of a murine protein binds 5' to its own homeo box. Proc Natl Acad Sci U S A. 1986 Dec;83(24):9532–9536. [PMC free article] [PubMed]
  • Coulson A, Sulston J, Brenner S, Karn J. Toward a physical map of the genome of the nematode Caenorhabditis elegans. Proc Natl Acad Sci U S A. 1986 Oct;83(20):7821–7825. [PMC free article] [PubMed]
  • Coulson A, Waterston R, Kiff J, Sulston J, Kohara Y. Genome linking with yeast artificial chromosomes. Nature. 1988 Sep 8;335(6186):184–186. [PubMed]
  • McGinnis W, Garber RL, Wirz J, Kuroiwa A, Gehring WJ. A homologous protein-coding sequence in Drosophila homeotic genes and its conservation in other metazoans. Cell. 1984 Jun;37(2):403–408. [PubMed]
  • Sanger F, Nicklen S, Coulson AR. DNA sequencing with chain-terminating inhibitors. Proc Natl Acad Sci U S A. 1977 Dec;74(12):5463–5467. [PMC free article] [PubMed]
  • McGinnis W, Levine MS, Hafen E, Kuroiwa A, Gehring WJ. A conserved DNA sequence in homoeotic genes of the Drosophila Antennapedia and bithorax complexes. Nature. 308(5958):428–433. [PubMed]
  • Scott MP, Weiner AJ. Structural relationships among genes that control development: sequence homology between the Antennapedia, Ultrabithorax, and fushi tarazu loci of Drosophila. Proc Natl Acad Sci U S A. 1984 Jul;81(13):4115–4119. [PMC free article] [PubMed]
  • Müller MM, Carrasco AE, DeRobertis EM. A homeo-box-containing gene expressed during oogenesis in Xenopus. Cell. 1984 Nov;39(1):157–162. [PubMed]
  • Desai C, Garriga G, McIntire SL, Horvitz HR. A genetic pathway for the development of the Caenorhabditis elegans HSN motor neurons. Nature. 1988 Dec 15;336(6200):638–646. [PubMed]
  • Mlodzik M, Fjose A, Gehring WJ. Molecular structure and spatial expression of a homeobox gene from the labial region of the Antennapedia-complex. EMBO J. 1988 Aug;7(8):2569–2578. [PMC free article] [PubMed]
  • Cohen SM, Brönner G, Küttner F, Jürgens G, Jäckle H. Distal-less encodes a homoeodomain protein required for limb development in Drosophila. Nature. 1989 Mar 30;338(6214):432–434. [PubMed]
  • Diederich RJ, Merrill VK, Pultz MA, Kaufman TC. Isolation, structure, and expression of labial, a homeotic gene of the Antennapedia Complex involved in Drosophila head development. Genes Dev. 1989 Mar;3(3):399–414. [PubMed]
  • Celniker SE, Keelan DJ, Lewis EB. The molecular genetics of the bithorax complex of Drosophila: characterization of the products of the Abdominal-B domain. Genes Dev. 1989 Sep;3(9):1424–1436. [PubMed]
  • Kim Y, Nirenberg M. Drosophila NK-homeobox genes. Proc Natl Acad Sci U S A. 1989 Oct;86(20):7716–7720. [PMC free article] [PubMed]
  • Baron A, Featherstone MS, Hill RE, Hall A, Galliot B, Duboule D. Hox-1.6: a mouse homeo-box-containing gene member of the Hox-1 complex. EMBO J. 1987 Oct;6(10):2977–2986. [PMC free article] [PubMed]
  • Hoey T, Doyle HJ, Harding K, Wedeen C, Levine M. Homeo box gene expression in anterior and posterior regions of the Drosophila embryo. Proc Natl Acad Sci U S A. 1986 Jul;83(13):4809–4813. [PMC free article] [PubMed]
  • Kamb A, Weir M, Rudy B, Varmus H, Kenyon C. Identification of genes from pattern formation, tyrosine kinase, and potassium channel families by DNA amplification. Proc Natl Acad Sci U S A. 1989 Jun;86(12):4372–4376. [PMC free article] [PubMed]
  • Hanes SD, Brent R. DNA specificity of the bicoid activator protein is determined by homeodomain recognition helix residue 9. Cell. 1989 Jun 30;57(7):1275–1283. [PubMed]

Articles from Nucleic Acids Research are provided here courtesy of Oxford University Press

Formats:

Related citations in PubMed

See reviews...See all...

Cited by other articles in PMC

See all...

Links

Recent Activity

Your browsing activity is empty.

Activity recording is turned off.

Turn recording back on

See more...