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Nucleic Acids Res. Mar 11, 1990; 18(5): 1121–1127.
PMCID: PMC330424

The human minisatellite consensus at breakpoints of oncogene translocations.

Abstract

A reexamination of human minisatellite (hypervariable) regions following the cloning and sequencing of the new minisatellite, VTR1.1, revealed that many of these structures possessed a strongly conserved copy of the chi-like octamer, GC[A/T]GG[A/T]GG. In oncogene translocations apparently created by aberrant VDJ recombinase activity, this VTR octamer was often found within a few bases of the breakpoint (p less than 10(-10)). Three bcl2 rearrangements which occurred within 2 bp of one another were located precisely adjacent to this consensus; it defined the 5' border of that oncogene's major breakpoint cluster. Several c-myc translocations also occurred within 2 bp of this sequence. While the appearance of a chi-like element in polymorphic minisatellite sequences is consistent with a role promoting either recombination or replication slippage, the existence of such elements at sites of somatic translocations suggests chi function in site-specific recombination, perhaps as a subsidiary recognition signal in immunoglobulin gene rearrangement. We discuss the implications of these observations for mechanisms by which oncogene translocations and minisatellite sequences are generated.

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Selected References

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  • Jeffreys AJ, Wilson V, Thein SL. Hypervariable 'minisatellite' regions in human DNA. Nature. 1985 Mar 7;314(6006):67–73. [PubMed]
  • Wetton JH, Carter RE, Parkin DT, Walters D. Demographic study of a wild house sparrow population by DNA fingerprinting. Nature. 1987 May 14;327(6118):147–149. [PubMed]
  • Jeffreys AJ, Wilson V, Kelly R, Taylor BA, Bulfield G. Mouse DNA 'fingerprints': analysis of chromosome localization and germ-line stability of hypervariable loci in recombinant inbred strains. Nucleic Acids Res. 1987 Apr 10;15(7):2823–2836. [PMC free article] [PubMed]
  • Kominami R, Mitani K, Muramatsu M. Nucleotide sequence of a mouse minisatellite DNA. Nucleic Acids Res. 1988 Feb 11;16(3):1197–1197. [PMC free article] [PubMed]
  • Georges M, Lequarré AS, Castelli M, Hanset R, Vassart G. DNA fingerprinting in domestic animals using four different minisatellite probes. Cytogenet Cell Genet. 1988;47(3):127–131. [PubMed]
  • Smith GR. Chi hotspots of generalized recombination. Cell. 1983 Oct;34(3):709–710. [PubMed]
  • Colb M, Yang-Feng T, Francke U, Mermer B, Parkinson DR, Krontiris TG. A variable tandem repeat locus mapped to chromosome band 10q26 is amplified and rearranged in leukocyte DNAs of two cancer patients. Nucleic Acids Res. 1986 Oct 24;14(20):7929–7937. [PMC free article] [PubMed]
  • Takahashi N, Ueda S, Obata M, Nikaido T, Nakai S, Honjo T. Structure of human immunoglobulin gamma genes: implications for evolution of a gene family. Cell. 1982 Jun;29(2):671–679. [PubMed]
  • Eckert RL, Green H. Structure and evolution of the human involucrin gene. Cell. 1986 Aug 15;46(4):583–589. [PubMed]
  • Azen E, Lyons KM, McGonigal T, Barrett NL, Clements LS, Maeda N, Vanin EF, Carlson DM, Smithies O. Clones from the human gene complex coding for salivary proline-rich proteins. Proc Natl Acad Sci U S A. 1984 Sep;81(17):5561–5565. [PMC free article] [PubMed]
  • Weller P, Jeffreys AJ, Wilson V, Blanchetot A. Organization of the human myoglobin gene. EMBO J. 1984 Feb;3(2):439–446. [PMC free article] [PubMed]
  • Silva AJ, Johnson JP, White RL. Characterization of a highly polymorphic region 5' to JH in the human immunoglobulin heavy chain. Nucleic Acids Res. 1987 May 11;15(9):3845–3857. [PMC free article] [PubMed]
  • Tsujimoto Y, Jaffe E, Cossman J, Gorham J, Nowell PC, Croce CM. Clustering of breakpoints on chromosome 11 in human B-cell neoplasms with the t(11;14) chromosome translocation. Nature. 1985 May 23;315(6017):340–343. [PubMed]
  • Boehm T, Buluwela L, Williams D, White L, Rabbitts TH. A cluster of chromosome 11p13 translocations found via distinct D-D and D-D-J rearrangements of the human T cell receptor delta chain gene. EMBO J. 1988 Jul;7(7):2011–2017. [PMC free article] [PubMed]
  • Haluska FG, Tsujimoto Y, Croce CM. The t(8;14) breakpoint of the EW 36 undifferentiated lymphoma cell line lies 5' of MYC in a region prone to involvement in endemic Burkitt's lymphomas. Nucleic Acids Res. 1988 Mar 25;16(5):2077–2085. [PMC free article] [PubMed]
  • Moulding C, Rapoport A, Goldman P, Battey J, Lenoir GM, Leder P. Structural analysis of both products of a reciprocal translocation between c-myc and immunoglobulin loci in Burkitt lymphoma. Nucleic Acids Res. 1985 Mar 25;13(6):2141–2152. [PMC free article] [PubMed]
  • Showe LC, Ballantine M, Nishikura K, Erikson J, Kaji H, Croce CM. Cloning and sequencing of a c-myc oncogene in a Burkitt's lymphoma cell line that is translocated to a germ line alpha switch region. Mol Cell Biol. 1985 Mar;5(3):501–509. [PMC free article] [PubMed]
  • Saito H, Hayday AC, Wiman K, Hayward WS, Tonegawa S. Activation of the c-myc gene by translocation: a model for translational control. Proc Natl Acad Sci U S A. 1983 Dec;80(24):7476–7480. [PMC free article] [PubMed]
  • Gelmann EP, Psallidopoulos MC, Papas TS, Dalla-Favera R. Identification of reciprocal translocation sites within the c-myc oncogene and immunoglobulin mu locus in a Burkitt lymphoma. Nature. 1983 Dec 22;306(5945):799–803. [PubMed]
  • Dyson PJ, Rabbitts TH. Chromatin structure around the c-myc gene in Burkitt lymphomas with upstream and downstream translocation points. Proc Natl Acad Sci U S A. 1985 Apr;82(7):1984–1988. [PMC free article] [PubMed]
  • Dalla-Favera R, Martinotti S, Gallo RC, Erikson J, Croce CM. Translocation and rearrangements of the c-myc oncogene locus in human undifferentiated B-cell lymphomas. Science. 1983 Feb 25;219(4587):963–967. [PubMed]
  • Wiman KG, Clarkson B, Hayday AC, Saito H, Tonegawa S, Hayward WS. Activation of a translocated c-myc gene: role of structural alterations in the upstream region. Proc Natl Acad Sci U S A. 1984 Nov;81(21):6798–6802. [PMC free article] [PubMed]
  • Hollis GF, Mitchell KF, Battey J, Potter H, Taub R, Lenoir GM, Leder P. A variant translocation places the lambda immunoglobulin genes 3' to the c-myc oncogene in Burkitt's lymphoma. Nature. 1984 Feb 23;307(5953):752–755. [PubMed]
  • Tsujimoto Y, Gorham J, Cossman J, Jaffe E, Croce CM. The t(14;18) chromosome translocations involved in B-cell neoplasms result from mistakes in VDJ joining. Science. 1985 Sep 27;229(4720):1390–1393. [PubMed]
  • Gauwerky CE, Haluska FG, Tsujimoto Y, Nowell PC, Croce CM. Evolution of B-cell malignancy: pre-B-cell leukemia resulting from MYC activation in a B-cell neoplasm with a rearranged BCL2 gene. Proc Natl Acad Sci U S A. 1988 Nov;85(22):8548–8552. [PMC free article] [PubMed]
  • Cleary ML, Sklar J. Nucleotide sequence of a t(14;18) chromosomal breakpoint in follicular lymphoma and demonstration of a breakpoint-cluster region near a transcriptionally active locus on chromosome 18. Proc Natl Acad Sci U S A. 1985 Nov;82(21):7439–7443. [PMC free article] [PubMed]
  • Bakhshi A, Wright JJ, Graninger W, Seto M, Owens J, Cossman J, Jensen JP, Goldman P, Korsmeyer SJ. Mechanism of the t(14;18) chromosomal translocation: structural analysis of both derivative 14 and 18 reciprocal partners. Proc Natl Acad Sci U S A. 1987 Apr;84(8):2396–2400. [PMC free article] [PubMed]
  • Murre C, McCaw PS, Baltimore D. A new DNA binding and dimerization motif in immunoglobulin enhancer binding, daughterless, MyoD, and myc proteins. Cell. 1989 Mar 10;56(5):777–783. [PubMed]
  • Cleary ML, Smith SD, Sklar J. Cloning and structural analysis of cDNAs for bcl-2 and a hybrid bcl-2/immunoglobulin transcript resulting from the t(14;18) translocation. Cell. 1986 Oct 10;47(1):19–28. [PubMed]
  • Showe LC, Croce CM. The role of chromosomal translocations in B- and T-cell neoplasia. Annu Rev Immunol. 1987;5:253–277. [PubMed]
  • Kenter AL, Birshtein BK. Chi, a promoter of generalized recombination in lambda phage, is present in immunoglobulin genes. Nature. 1981 Oct 1;293(5831):402–404. [PubMed]
  • Yancopoulos GD, Alt FW. Regulation of the assembly and expression of variable-region genes. Annu Rev Immunol. 1986;4:339–368. [PubMed]
  • Haluska FG, Tsujimoto Y, Croce CM. The t(8;14) chromosome translocation of the Burkitt lymphoma cell line Daudi occurred during immunoglobulin gene rearrangement and involved the heavy chain diversity region. Proc Natl Acad Sci U S A. 1987 Oct;84(19):6835–6839. [PMC free article] [PubMed]
  • Wiggs J, Nordenskjöld M, Yandell D, Rapaport J, Grondin V, Janson M, Werelius B, Petersen R, Craft A, Riedel K, et al. Prediction of the risk of hereditary retinoblastoma, using DNA polymorphisms within the retinoblastoma gene. N Engl J Med. 1988 Jan 21;318(3):151–157. [PubMed]
  • Tutter A, Riblet R. Conservation of an immunoglobulin variable-region gene family indicates a specific, noncoding function. Proc Natl Acad Sci U S A. 1989 Oct;86(19):7460–7464. [PMC free article] [PubMed]
  • Kasperczyk A, Mermer BA, Parkinson DR, Lonergan JA, Krontiris TG. Allele-specific deletion in exon I of the HRAS1 gene. Am J Hum Genet. 1989 Nov;45(5):689–696. [PMC free article] [PubMed]
  • Ludwig EH, Friedl W, McCarthy BJ. High-resolution analysis of a hypervariable region in the human apolipoprotein B gene. Am J Hum Genet. 1989 Sep;45(3):458–464. [PMC free article] [PubMed]

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