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J Clin Invest. Oct 1989; 84(4): 1160–1166.
PMCID: PMC329773

Erythropoietin in polycystic kidneys.

Abstract

Erythropoietin (EPO) formation in kidneys of 18 patients with autosomal dominant polycystic kidney disease (ADPKD) was investigated. In 12 patients on hemodialysis and in 6 patients with preterminal renal failure serum, EPO was 29 +/- 7 and 16 +/- 1.5 mU/ml and hemoglobin concentrations were 11.0 +/- 0.6 and 12.7 +/- 1.2 g/dl, respectively. Cyst fluid from a total of 357 renal cysts was obtained by either in vivo aspiration or immediately after nephrectomy. The cysts contained variable concentrations of bioactive EPO from undectable values up to 3.2 U/ml. A pronounced enrichment of EPO was observed in cysts with sodium concentrations greater than 100 mmol/liter, suggesting an association with proximal tubular malformations. The EPO concentrations in the cysts were neither correlated with the protein concentration nor with the oxygen pressure of the cyst fluid. Using a cDNA probe for human EPO, mRNA for EPO was localized in stroma cells of the cyst walls by an in situ hybridization technique. Our findings suggest that single interstitial cells juxtaposed to proximal tubular cysts may produce EPO independent of the oxygen pressure inside the cysts, which ameliorates the anemia during end-stage polycystic kidney disease.

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  • Eschbach JW, Adamson JW. Modern aspects of the pathophysiology of renal anemia. Contrib Nephrol. 1988;66:63–70. [PubMed]
  • Chandra M, Miller ME, Garcia JF, Mossey RT, McVicar M. Serum immunoreactive erythropoietin levels in patients with polycystic kidney disease as compared with other hemodialysis patients. Nephron. 1985;39(1):26–29. [PubMed]
  • Besarab A, Caro J, Jarrell BE, Francos G, Erslev AJ. Dynamics of erythropoiesis following renal transplantation. Kidney Int. 1987 Oct;32(4):526–536. [PubMed]
  • Pavlović-Kentera V, Clemons GK, Djukanović L, Biljanović-Paunovic L. Erythropoietin and anemia in chronic renal failure. Exp Hematol. 1987 Aug;15(7):785–789. [PubMed]
  • ROSSE WF, WALDMANN TA, COHEN P. Renal cysts, erythropoietin and polycythemia. Am J Med. 1963 Jan;34:76–81. [PubMed]
  • Koplan JP, Sprayregan S, Ossias AL, Zanjani ED. Erythropoietin-producing renal cyst and polycythemia vera. Clarification of their relationship. Am J Med. 1973 Jun;54(6):819–824. [PubMed]
  • Eckardt KU, Kurtz A, Hirth P, Scigalla P, Wieczorek L, Bauer C. Evaluation of the stability of human erythropoietin in samples for radioimmunoassay. Klin Wochenschr. 1988 Mar 15;66(6):241–245. [PubMed]
  • Laemmli UK. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970 Aug 15;227(5259):680–685. [PubMed]
  • LOWRY OH, ROSEBROUGH NJ, FARR AL, RANDALL RJ. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed]
  • Rigby PW, Dieckmann M, Rhodes C, Berg P. Labeling deoxyribonucleic acid to high specific activity in vitro by nick translation with DNA polymerase I. J Mol Biol. 1977 Jun 15;113(1):237–251. [PubMed]
  • Tourtellotte WW, Verity AN, Schmid P, Martinez S, Shapshak P. Covalent binding of formalin fixed paraffin embedded brain tissue sections to glass slides suitable for in situ hybridization. J Virol Methods. 1987 Feb;15(2):87–99. [PubMed]
  • Gardner KD., Jr Composition of fluid in twelve cysts of a polycystic kidney. N Engl J Med. 1969 Oct 30;281(18):985–988. [PubMed]
  • Huseman R, Grady A, Welling D, Grantham J. Macropuncture study of polycystic disease in adult human kidneys. Kidney Int. 1980 Sep;18(3):375–385. [PubMed]
  • Bennett WM, Elzinga L, Pulliam JP, Rashad AL, Barry JM. Cyst fluid antibiotic concentrations in autosomal-dominant polycystic kidney disease. Am J Kidney Dis. 1985 Dec;6(6):400–404. [PubMed]
  • Muther RS, Bennett WM. Cyst fluid antibiotic concentrations in polycystic kidney disease: differences between proximal and distal cysts. Kidney Int. 1981 Oct;20(4):519–522. [PubMed]
  • Baert L. Hereditary polycystic kidney disease (adult form): a microdissection study of two cases at an early stage of the disease. Kidney Int. 1978 Jun;13(6):519–525. [PubMed]
  • BRICKER NS, PATTON JF. Cystic disease of the kidneys; a study of dynamics and chemical composition of cyst fluid. Am J Med. 1955 Feb;18(2):207–219. [PubMed]
  • Grantham JJ, Geiser JL, Evan AP. Cyst formation and growth in autosomal dominant polycystic kidney disease. Kidney Int. 1987 May;31(5):1145–1152. [PubMed]
  • Hoffman WS, Kozoll DD. THE FATE OF INTRAVENOUSLY INJECTED GELATIN IN HUMAN SUBJECTS. J Clin Invest. 1946 Jul;25(4):575–585. [PMC free article] [PubMed]
  • Lacombe C, Da Silva JL, Bruneval P, Fournier JG, Wendling F, Casadevall N, Camilleri JP, Bariety J, Varet B, Tambourin P. Peritubular cells are the site of erythropoietin synthesis in the murine hypoxic kidney. J Clin Invest. 1988 Feb;81(2):620–623. [PMC free article] [PubMed]
  • Koury ST, Bondurant MC, Koury MJ. Localization of erythropoietin synthesizing cells in murine kidneys by in situ hybridization. Blood. 1988 Feb;71(2):524–527. [PubMed]
  • Birenboim N, Donoso VS, Huseman RA, Grantham JJ. Renal excretion and cyst accumulation of beta 2microglobulin in polycystic kidney disease. Kidney Int. 1987 Jan;31(1):85–92. [PubMed]
  • Eckardt KU, Kurtz A, Bauer C. Regulation of erythropoietin production is related to proximal tubular function. Am J Physiol. 1989 May;256(5 Pt 2):F942–F947. [PubMed]
  • Kurtz A, Eckardt KE, Tannahill L, Bauer C. Regulation of erythropoietin production. Contrib Nephrol. 1988;66:1–16. [PubMed]
  • Miller ME, Rorth M, Parving HH, Howard D, Reddington I, Valeri CR, Stohlman F., Jr pH effect on erythropoietin response to hypoxia. N Engl J Med. 1973 Apr 5;288(14):706–710. [PubMed]
  • Erslev AJ, Wilson J, Caro J. Erythropoietin titers in anemic, nonuremic patients. J Lab Clin Med. 1987 Apr;109(4):429–433. [PubMed]

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