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Nucleic Acids Res. May 11, 1991; 19(9): 2321–2323.
PMCID: PMC329437

BsiY I, a novel thermophilic restriction endonuclease that recognizes 5' CCNNNNNNNGG 3' and the discovery of a wrongly sequenced site in pACYC177.

Abstract

A new type II restriction endonuclease designated BsiY I has been purified from a thermophilic soil Bacillus stearothermophilus strain. This enzyme recognizes and cleaves the highly degenerate sequence 5' CCNNNNN!NNGG 3'. During the identification of the recognition sequence of BsiY I, we discovered that there should be five G nucleotides instead of four at position 1227-1230 of the plasmid pACYC177.

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Selected References

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  • Mok YK, Clark DR, Kam KM, Shaw PC. Restriction endonuclease from thermophilic bacterial species I. Isolation and characterization of BsiEI. Nucleic Acids Res. 1990 Aug 25;18(16):4954–4954. [PMC free article] [PubMed]
  • Mok YK, Clark DR, Kam KM, Shaw PC. Restriction endonuclease from thermophilic bacterial species. II. Isolation and characterization of BsiBI. Nucleic Acids Res. 1990 Nov 25;18(22):6740–6740. [PMC free article] [PubMed]
  • Selzer G, Som T, Itoh T, Tomizawa J. The origin of replication of plasmid p15A and comparative studies on the nucleotide sequences around the origin of related plasmids. Cell. 1983 Jan;32(1):119–129. [PubMed]
  • Rose RE. The nucleotide sequence of pACYC177. Nucleic Acids Res. 1988 Jan 11;16(1):356–356. [PMC free article] [PubMed]
  • Rose RE. The nucleotide sequence of pACYC184. Nucleic Acids Res. 1988 Jan 11;16(1):355–355. [PMC free article] [PubMed]
  • Brown NL, Smith M. A general method for defining restriction enzyme cleavage and recognition sites. Methods Enzymol. 1980;65(1):391–404. [PubMed]
  • Shaw PC, Yung MH, Zhu RH, Ho WK, Ng TB, Yeung HW. Cloning of trichosanthin cDNA and its expression in Escherichia coli. Gene. 1991 Jan 15;97(2):267–272. [PubMed]
  • Roberts RJ. Restriction enzymes and their isoschizomers. Nucleic Acids Res. 1990 Apr 25;18 (Suppl):2331–2365. [PMC free article] [PubMed]
  • Kessler C, Manta V. Specificity of restriction endonucleases and DNA modification methyltransferases a review (Edition 3). Gene. 1990 Aug 16;92(1-2):1–248. [PubMed]

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