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Nucleic Acids Res. Dec 25, 1991; 19(24): 6763–6769.
PMCID: PMC329307

Tomato yellow leaf curl virus from Sardinia is a whitefly-transmitted monopartite geminivirus.

Abstract

The genome of an isolate of tomato yellow leaf curl virus from Sardinia, Italy (TYLCV-S), a geminivirus transmitted by the whitefly Bemisia tabaci, has been cloned and sequenced. The single circular DNA molecule comprises 2770 nucleotides. Genome organisation closely resembles that of the DNA A component of the whitefly-transmitted geminiviruses with a bipartite genome. A 1.8 mer of the TYLCV-S genome in a binary vector of Agrobacterium tumefaciens is infectious upon agroinoculation of tomato plants. Typical tomato yellow leaf curl disease symptoms developed about three weeks after inoculation. The disease was transmitted by the natural vector B.tabaci from agroinfected plants to test plants, reproducing in this way the full biological cycle and proving that the genome of TYLCV-S consists of only one circular single-stranded DNA molecule. Contrary to the other whitefly-transmitted geminiviruses described so far, there is no evidence for the existence nor the necessity of a second component (B DNA) in the TYLCV-S genome.

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Selected References

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  • Stanley J. The molecular biology of geminiviruses. Adv Virus Res. 1985;30:139–177. [PubMed]
  • Davies JW, Stanley J. Geminivirus genes and vectors. Trends Genet. 1989 Mar;5(3):77–81. [PubMed]
  • Rogers SG, Bisaro DM, Horsch RB, Fraley RT, Hoffmann NL, Brand L, Elmer JS, Lloyd AM. Tomato golden mosaic virus A component DNA replicates autonomously in transgenic plants. Cell. 1986 May 23;45(4):593–600. [PubMed]
  • Townsend R, Watts J, Stanley J. Synthesis of viral DNA forms in Nicotiana plumbaginifolia protoplasts inoculated with cassava latent virus (CLV); evidence for the independent replication of one component of the CLV genome. Nucleic Acids Res. 1986 Feb 11;14(3):1253–1265. [PMC free article] [PubMed]
  • Elmer JS, Brand L, Sunter G, Gardiner WE, Bisaro DM, Rogers SG. Genetic analysis of the tomato golden mosaic virus. II. The product of the AL1 coding sequence is required for replication. Nucleic Acids Res. 1988 Jul 25;16(14B):7043–7060. [PMC free article] [PubMed]
  • Rochester DE, Kositratana W, Beachy RN. Systemic movement and symptom production following agroinoculation with a single DNA of tomato yellow leaf curl geminivirus (Thailand). Virology. 1990 Oct;178(2):520–526. [PubMed]
  • Rigby PW, Dieckmann M, Rhodes C, Berg P. Labeling deoxyribonucleic acid to high specific activity in vitro by nick translation with DNA polymerase I. J Mol Biol. 1977 Jun 15;113(1):237–251. [PubMed]
  • Vieira J, Messing J. Production of single-stranded plasmid DNA. Methods Enzymol. 1987;153:3–11. [PubMed]
  • Sanger F, Nicklen S, Coulson AR. DNA sequencing with chain-terminating inhibitors. Proc Natl Acad Sci U S A. 1977 Dec;74(12):5463–5467. [PMC free article] [PubMed]
  • Bevan M. Binary Agrobacterium vectors for plant transformation. Nucleic Acids Res. 1984 Nov 26;12(22):8711–8721. [PMC free article] [PubMed]
  • Höfgen R, Willmitzer L. Storage of competent cells for Agrobacterium transformation. Nucleic Acids Res. 1988 Oct 25;16(20):9877–9877. [PMC free article] [PubMed]
  • Ooms G, Hooykaas PJ, Van Veen RJ, Van Beelen P, Regensburg-Tuïnk TJ, Schilperoort RA. Octopine Ti-plasmid deletion mutants of agrobacterium tumefaciens with emphasis on the right side of the T-region. Plasmid. 1982 Jan;7(1):15–29. [PubMed]
  • Matzeit V, Schaefer S, Kammann M, Schalk HJ, Schell J, Gronenborn B. Wheat dwarf virus vectors replicate and express foreign genes in cells of monocotyledonous plants. Plant Cell. 1991 Mar;3(3):247–258. [PMC free article] [PubMed]
  • Feinberg AP, Vogelstein B. A technique for radiolabeling DNA restriction endonuclease fragments to high specific activity. Anal Biochem. 1983 Jul 1;132(1):6–13. [PubMed]
  • Stanley J, Markham PG, Callis RJ, Pinner MS. The nucleotide sequence of an infectious clone of the geminivirus beet curly top virus. EMBO J. 1986 Aug;5(8):1761–1767. [PMC free article] [PubMed]
  • Gay NJ, Walker JE. Homology between human bladder carcinoma oncogene product and mitochondrial ATP-synthase. Nature. 1983 Jan 20;301(5897):262–264. [PubMed]
  • A simple and general method for transferring genes into plants. Science. 1985 Mar 8;227(4691):1229–1231. [PubMed]
  • Rybicki EP, Hughes FL. Detection and typing of maize streak virus and other distantly related geminiviruses of grasses by polymerase chain reaction amplification of a conserved viral sequence. J Gen Virol. 1990 Nov;71(Pt 11):2519–2526. [PubMed]
  • Hamilton WD, Stein VE, Coutts RH, Buck KW. Complete nucleotide sequence of the infectious cloned DNA components of tomato golden mosaic virus: potential coding regions and regulatory sequences. EMBO J. 1984 Sep;3(9):2197–2205. [PMC free article] [PubMed]
  • Howarth AJ, Caton J, Bossert M, Goodman RM. Nucleotide sequence of bean golden mosaic virus and a model for gene regulation in geminiviruses. Proc Natl Acad Sci U S A. 1985 Jun;82(11):3572–3576. [PMC free article] [PubMed]
  • Navot N, Pichersky E, Zeidan M, Zamir D, Czosnek H. Tomato yellow leaf curl virus: a whitefly-transmitted geminivirus with a single genomic component. Virology. 1991 Nov;185(1):151–161. [PubMed]
  • Morris B, Coates L, Lowe S, Richardson K, Eddy P. Nucleotide sequence of the infectious cloned DNA components of African cassava mosaic virus (Nigerian strain). Nucleic Acids Res. 1990 Jan 11;18(1):197–198. [PMC free article] [PubMed]
  • Lazarowitz SG. Infectivity and complete nucleotide sequence of the genome of a South African isolate of maize streak virus. Nucleic Acids Res. 1988 Jan 11;16(1):229–249. [PMC free article] [PubMed]
  • Briddon RW, Pinner MS, Stanley J, Markham PG. Geminivirus coat protein gene replacement alters insect specificity. Virology. 1990 Jul;177(1):85–94. [PubMed]
  • Lazarowitz SG, Lazdins IB. Infectivity and complete nucleotide sequence of the cloned genomic components of a bipartite squash leaf curl geminivirus with a broad host range phenotype. Virology. 1991 Jan;180(1):58–69. [PubMed]
  • Boulton MI, Steinkellner H, Donson J, Markham PG, King DI, Davies JW. Mutational analysis of the virion-sense genes of maize streak virus. J Gen Virol. 1989 Sep;70(Pt 9):2309–2323. [PubMed]
  • Lazarowitz SG, Pinder AJ, Damsteegt VD, Rogers SG. Maize streak virus genes essential for systemic spread and symptom development. EMBO J. 1989 Apr;8(4):1023–1032. [PMC free article] [PubMed]
  • Etessami P, Watts J, Stanley J. Size reversion of African cassava mosaic virus coat protein gene deletion mutants during infection of Nicotiana benthamiana. J Gen Virol. 1989 Feb;70(Pt 2):277–289. [PubMed]
  • Howarth AJ, Vandemark GJ. Phylogeny of geminiviruses. J Gen Virol. 1989 Oct;70(Pt 10):2717–2727. [PubMed]
  • Devereux J, Haeberli P, Smithies O. A comprehensive set of sequence analysis programs for the VAX. Nucleic Acids Res. 1984 Jan 11;12(1 Pt 1):387–395. [PMC free article] [PubMed]
  • Hanley-Bowdoin L, Elmer JS, Rogers SG. Expression of functional replication protein from tomato golden mosaic virus in transgenic tobacco plants. Proc Natl Acad Sci U S A. 1990 Feb;87(4):1446–1450. [PMC free article] [PubMed]
  • Hayes RJ, Buck KW. Replication of tomato golden mosaic virus DNA B in transgenic plants expressing open reading frames (ORFs) of DNA A: requirement of ORF AL2 for production of single-stranded DNA. Nucleic Acids Res. 1989 Dec 25;17(24):10213–10222. [PMC free article] [PubMed]
  • Sunter G, Hartitz MD, Hormuzdi SG, Brough CL, Bisaro DM. Genetic analysis of tomato golden mosaic virus: ORF AL2 is required for coat protein accumulation while ORF AL3 is necessary for efficient DNA replication. Virology. 1990 Nov;179(1):69–77. [PubMed]
  • Sunter G, Bisaro DM. Transactivation in a geminivirus: AL2 gene product is needed for coat protein expression. Virology. 1991 Jan;180(1):416–419. [PubMed]
  • Etessami P, Saunders K, Watts J, Stanley J. Mutational analysis of complementary-sense genes of African cassava mosaic virus DNA A. J Gen Virol. 1991 May;72(Pt 5):1005–1012. [PubMed]
  • Revington GN, Sunter G, Bisaro DM. DNA sequences essential for replication of the B genome component of tomato golden mosaic virus. Plant Cell. 1989 Oct;1(10):985–992. [PMC free article] [PubMed]
  • Stanley J, Frischmuth T, Ellwood S. Defective viral DNA ameliorates symptoms of geminivirus infection in transgenic plants. Proc Natl Acad Sci U S A. 1990 Aug;87(16):6291–6295. [PMC free article] [PubMed]
  • Kikuno R, Toh H, Hayashida H, Miyata T. Sequence similarity between putative gene products of geminiviral DNAs. Nature. 1984 Apr 5;308(5959):562–562. [PubMed]
  • Frischmuth T, Zimmat G, Jeske H. The nucleotide sequence of abutilon mosaic virus reveals prokaryotic as well as eukaryotic features. Virology. 1990 Oct;178(2):461–468. [PubMed]

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