• We are sorry, but NCBI web applications do not support your browser and may not function properly. More information
Logo of plosonePLoS OneView this ArticleSubmit to PLoSGet E-mail AlertsContact UsPublic Library of Science (PLoS)
PLoS One. 2012; 7(2): e32183.
Published online Feb 16, 2012. doi:  10.1371/journal.pone.0032183
PMCID: PMC3281126

C. elegans VANG-1 Modulates Life Span via Insulin/IGF-1-Like Signaling

Anne C. Hart, Editor

Abstract

The planar cell polarity (PCP) pathway is highly conserved from Drosophila to humans and a PCP-like pathway has recently been described in the nematode Caenorhabditis elegans. The developmental function of this pathway is to coordinate the orientation of cells or structures within the plane of an epithelium or to organize cell-cell intercalation required for correct morphogenesis. Here, we describe a novel role of VANG-1, the only C. elegans ortholog of the conserved PCP component Strabismus/Van Gogh. We show that two alleles of vang-1 and depletion of the protein by RNAi cause an increase of mean life span up to 40%. Consistent with the longevity phenotype vang-1 animals also show enhanced resistance to thermal- and oxidative stress and decreased lipofuscin accumulation. In addition, vang-1 mutants show defects like reduced brood size, decreased ovulation rate and prolonged reproductive span, which are also related to gerontogenes. The germline, but not the intestine or neurons, seems to be the primary site of vang-1 function. Life span extension in vang-1 mutants depends on the insulin/IGF-1-like receptor DAF-2 and DAF-16/FoxO transcription factor. RNAi against the phase II detoxification transcription factor SKN-1/Nrf2 also reduced vang-1 life span that might be explained by gradual inhibition of insulin/IGF-1-like signaling in vang-1. This is the first time that a key player of the PCP pathway is shown to be involved in the insulin/IGF-1-like signaling dependent modulation of life span in C. elegans.

Introduction

Wnt/planar cell polarity (PCP) is one of three identified Wnt signaling pathways, along with Wnt/ß–Catenin and Wnt/Calcium [1]. These signaling pathways are abundant in various developmental processes across the animal kingdom [2][6]. PCP is extensively studied in the Drosophila wing, or in the organization of ommatidia in the fly eye or hair follicles in mammalian skin. Six proteins were placed in the core PCP pathway, Frizzled (Fz), Dishevelled (Dsh), Diego (Dgo), Strabismus/Van Gogh (Stbm/Vang), Prickle (Pk) and Flamingo (Fmi). The signaling mediated by PCP core proteins during development contributes to the polarization alongside the epithelial anterior-posterior or proximo-distal axis and requires contrary clustering of PCP components at the respective cell cortex. As a consequence of PCP signaling, downstream effectors (e.g., the actin cytoskeleton) are polarized within individual cells that finally lead to well organized structures within the two-dimensional epithelial surface [7], [8]. PCP processes also shape three-dimensional tissues that do not exhibit obvious signs of planar polarity. Here, individual cells have to move in a specific direction or divide with a specific orientation, hence showing transient planar polarization (e.g., during mediolateral cell intercalation) required for morphogenesis of the neural tube in vertebrates [9], [10]. Novel components of PCP signaling have been identified in the recent years, and the number of crosslinks to other conserved pathways required for development is rising [11][13].

The C. elegans genome (http://www.wormbase.org) encodes a sole four-pass transmembrane protein, VANG-1 showing sequence similarities and conservation of overall domain architecture compared to the Strabismus/Van Gogh/Ltap proteins identified in Drosophila, Xenopus and mammals. Like in Drosophila and mammals, VANG-1 contains four hydrophobic transmembrane domains at its N-terminus and a consensus PDZ binding motif at its C-terminus [13]. VANG-1 was implicated in playing a minor role in B cell polarity in the C. elegans male tail [14]. However, it plays a major role in organ formation either by mediating correct intercalation of intestinal primordial cells during embryogenesis [13], [15] or by establishing ground polarity in vulval development [12]. Whereas PCP signaling required for morphogenesis is generally well understood, a more physiological role of this pathway with effects on metabolism has not been described so far.

C. elegans is a well-established model to study genes that contribute to the process of aging. The corresponding genes of “Age” mutants are referred to as gerontogenes [16]. These mutants share a specific catalog of defects, e.g., a minimum of 20% life span increase and resistance against certain stress factors like reactive oxygen species or heat. The C. elegans homolog of insulin receptor in mammals, daf-2, is one of the best described gerontogenes, and the signaling mediated by DAF-2 is well understood [17][19]. DAF-2 is capable to phosphorylate target substrates, e.g., AGE-1/AAP-1, a PI3 kinase that generates PI(3,4,5)P3 [20][22]. Via a phosphorylation cascade, downstream kinases PDK-1, AKT-1, AKT-2 and SGK-1 [23][25] are activated and in turn negatively regulate the forkhead transcription factor (FoxO), DAF-16 [26], [27]. Inhibition of DAF-2 signaling (e.g., by daf-2 mutations or active insulin peptide signaling) leads to dephosphorylation, activation and accumulation of DAF-16 in the nucleus [28]. Consequently, transcription of DAF-16 targets that include genes involved in defence against stresses, DNA repair and metabolism lead to a higher resistance against stresses and significantly extension of life span [29]. Besides DAF-16, inhibiting insulin/IGF-1-like signaling also activates heat-shock transcription factor HSF-1 and phase II detoxification transcription factor SKN-1, a Nrf1/2/3 protein ortholog [30], [31].

In the present study, we identify VANG-1, the only C. elegans ortholog of the conserved PCP protein Strabismus/Van Gogh, as a gerontogene with a typical phenotype, including extended life- and reproductive span, multiple stress resistances, slow growth, reduced brood size and reduced lipofuscin accumulation. The vang-1–dependent life span extension and stress defences seem to be coordinated in the germline and mostly require daf-16 and skn-1 gene functions.

Results and Discussion

vang-1 increases life span, stress resistance and reproductive span in C. elegans

The C. elegans genome (http://www.wormbase.org) contains a sole four-pass transmembrane protein with homology to the Strabismus/Van Gogh/Ltap proteins identified in Drosophila, Xenopus and mammals [32][34]. During analysis of vang-1(tm1422), in which 188 amino acids of the N-terminus are missing (including three of the four transmembrane domains; see supporting information S1) [13], we noticed several defects (Figs. 1, ,2)2) with regard to the postembryonic phenotype, e.g., slow growth (data not shown) and reduced fecundity (Fig. 2A) that are also associated with loss of function phenotypes of certain aging genes in C. elegans [35]. Life span assays in vang-1(tm1422) at 25°C (Fig. 1A), 20°C and 18°C (Table 1) detected a significant increase in mean life span of up to 40% compared to wild type (WT) animals. Furthermore, we tested life span of another vang-1 deletion mutation, ok1142, lacking 162 amino acids of the C-terminus (including a predicted phosphorylation site; see supporting information S1) [13] and of animals depleted of VANG-1 by RNAi (Fig. 1A). Again, we noticed a significant extension of C. elegans mean life span up to 27% and 20% in comparison to WT controls either kept on standard OP50 or RNAi HT115 bacteria with the empty “feeding”-vector. In addition, the tm1422 phenotype was not enhanced by RNAi against vang-1 C-terminus (Table 1). With regard to longevity we assume tm1422 to be a null mutation whereas ok1142 seems to be a hypomorphic allele and RNAi does not generate the complete loss-of-function phenotype, as reported for other genes [36]. Hence, we used tm1422 in all further experiments.

Figure 1
vang-1 function interferes with life span extension and resistance against high temperature and reactive oxygen species in C. elegans.
Figure 2
vang-1 shows reproduction- and aging-related defects.
Table 1
Summary of life spans.

Next, we tested increased resistance of tm1422 against various stressors, a typical feature of gerontogenes. First, we measured thermoresistance in semi-automated and manual assays using SYTOX® Green nucleic acid stain under lethal temperature conditions of 37°C. Both assays revealed increased thermoresistance of tm1422 of about 40% (Fig. 1B and not shown), which is in the similar range as extension of mean life span. Second, we tested the resistance of tm1422 against reactive oxygen species (ROS) and determined intracellular ROS accumulation in living worms. In a stress assay with juglone (from Juglans niger) as a redox cycler, we found the fraction of tm1422 animals that survived the induced ROS stress conditions was about four fold higher than WT (Fig. 1C). Furthermore, a 60% decrease in ROS accumulation, which is in the similar range of daf-2 mutant population (Fig. 1D), was found in tm1422 worms in comparison to WT using a fluorescence well-plate reader to measure DCF fluorescence (see “methods” for details). According to the “free radical theory of aging” [37], ROS are a crucial factor for aging, and the intracellular amount of ROS can be correlated to stress. Organisms developed inducible detoxification systems like catalases, peroxidases and superoxide dismutases to reduce ROS levels [38]. The competence to keep intracellular ROS levels low is considered to be one possibility for the extension of life span [39], [40]. Thus, the diminished amounts of ROS in tm1422 may explain the increased survival rate at lethal thermal stress conditions. Recent findings suggest that the relationship between ROS and the aging process is more complex than what was originally thought. The generation of ROS cannot be longer seen as the initial trigger of the aging process [41]. Nevertheless, in case of tm1422 population reduced ROS generation indicates a lower stress level that finally may account for the extension of life span.

In order to estimate the biological age of vang-1 mutants, we measured the amount of lipofuscin, a product of oxidative damage and autophagy. In C. elegans, lipofuscin is detectable as autofluorescent granules in the intestine and its accumulation is a well-established marker to judge the biological age of C. elegans [42], [43]. In comparison to WT, ok1142 and tm1422 animals showed a significantly decreased accumulation of lipofuscin after five (Fig. 2B) and even after ten days (Fig. 2C).

In addition to the longevity phenotype, we observed a significantly reduced brood size in tm1422 animals (Fig. 2A), a decreased ovulation rate (Fig. 2D) and a dramatically prolonged reproductive span (Fig. 2E). Normally, the reproductive system of C. elegans ages significantly during the first week of adulthood [44], reflected by germline degeneration and a decline in oocyte quality [42], [45]. Individual tm1422 mothers continue to produce viable progeny as they age (Fig. 2D). This phenotype also points to vang-1 being a typical gerontogene. Some of the known mutations (e.g., daf-4 or daf-7) that extend C. elegans' reproductive period also regulate longevity, suggesting that there is a link between reproductive span and life span [46].

Taken together these results suggest that loss of the planar cell polarity ortholog VANG-1 causes robust temperature independent extension of life span, increases stress resistance and extends reproductive period in C. elegans.

Life span modulation by VANG-1 depends on the insulin/IGF-1-like signaling pathway

The main regulator of longevity and stress resistance in C. elegans is insulin/IGF-1-like signaling with its effector DAF-16. This FoxO transcription factor is translocated into the nucleus where it activates gene expression for distinct processes, e.g., resistance against different stressors and longevity when insulin/IGF-1-like signaling is inhibited [47], [48]. To gain further insight into the pathway operating in tm1422, we disrupted FoxO/DAF-16 transcription factor by RNAi in tm1422 and WT worms and compared the mean life span (Fig. 3A and Table 1). As expected [49], mean life span in WT animals depleted of DAF-16 slightly decreased in comparison to the control. Surprisingly, daf-16(RNAi) in tm1422 eliminated vang-1 induced life span extension at 20°C and 25°C (Table 1), suggesting that daf-16 is epistatic to vang-1.

Figure 3
vang-1(tm1422) life span modulation depends on Insulin/IGF-1-like signaling and leads to higher DAF-16 activity.

The activation of the DAF-16 transcription factor can be easily observed by a functional DAF-16::GFP fusion [50]. After vang-1(RNAi) at room temperature and 27°C we observed 16% and 57% DAF-16 translocation into the nucleus, respectively (Fig. S1), suggesting that complete nuclear localization of DAF-16 is not a prerequisite for increased life span and stress resistance. This phenomenon has also been observed in case of age-1 at 20°C, which is well known for modulating life span in a DAF-16 dependent manner [48], [50].

To further validate our daf-16(RNAi) life span result, we investigated other parameters of high DAF-16 activity (e.g., developmental arrest). In C. elegans, the activity of DAF-16 is sufficient and necessary for L1 diapause and dauer formation [25], [51]. Hatching L1 larvae stay in diapause, a developmental arrested state with reduced metabolism, until they start feeding. Dauer formation is an alternative third larval stage (beside the normal L3 larval stage) that is introduced under harsh environmental conditions, high temperature, low food or overcrowding [52].

We performed our dauer assay in comparison to WT, daf-2(e1370) and daf-16(mu86) at 27°C [53]. Consistent with the literature, we found that daf-2(e1370), encoding the sole insulin receptor homologue in C. elegans [20], is dauer constitutive (~99% arrest), while daf-16(mu86) is dauer defective (0% arrest; Fig. 3B) [51], [54]. tm1422 animals showed four times more developmental arrest compared to WT (Fig. 3B), which is inhibited by RNAi against daf-16 (tm1422: 7.8% dauer, 92.2% “other”, n = 64; WT: 1.2% dauer, 98.8% “other”, n = 160;). While 21% of WT animals developed into dauers, 58% and 18% of tm1422 animals arrested as dauers and in L1 diapause, respectively (Fig. 3B). A noteworthy difference concerning the dauer constitutive phenotypes of daf-2 and tm1422 is the percentage of L1 diapause arrests, which is also induced by DAF-16 [51] and suggests higher activity of DAF-16 in tm1422 during early development.

We further investigated the role of the receptor tyrosine kinase DAF-2 [20], which acts upstream of FoxO/DAF-16 transcription factor to modulate life span and stress resistance in the conserved insulin/IGF-1-like signaling pathway [55]. Inhibition or loss of DAF-2 function leads to induction of alternate dauer formation (see above) early in life and life span extension of up to 100% late in life both depending on DAF-16 function [29]. RNAi against daf-2 in WT and tm1422 worms resulted in nearly identical survival curves with no significant difference in mean life span (Fig. 3A and Table 1), indicating that vang-1 may function in the insulin/IGF-1-like signaling pathway, rather than in parallel pathways, e.g., through regulation of DAF-16 by kri-1 and lipophilic-hormone signaling [56], [57].

We also tested the longevity promoting factor SKN-1/Nrf2, which orchestrates the phase II detoxification response including defense against oxidative stress [58]. RNAi against skn-1 did reduce tm1422 life span significantly about 17% (Table 1). Inhibition of insulin/IGF-1-like signaling in tm1422 may explain this result. Like DAF-16, SKN-1 is also repressed by DAF-2 downstream kinases, AKT-1/2 and SGK-1 and possibly acts as a key player in a positive feedback loop to extend life span [58], [59].

To further specify how vang-1 functions in the extension of life span, we performed specific knock downs of vang-1 first in the intestine [60], [61], second in the germline [62] and third, because of its expression in ventral cord neurons [12], [63], in strains showing enhanced neuronal RNAi [64].

The intestine is highly exposed to environmental toxins and pathogens and it has been speculated to be the major site of stress response [65]. To further support this hypothesis, we depleted DAF-2 (as a control) by RNAi only in the intestine and found a 60% extension of life span (Table 1). In contrast, vang-1(RNAi) in the intestine did not result in a significant extension of mean life span (Fig. 4A; Table 1), suggesting that the intestine is not where VANG-1 is acting to modulate life span.

Figure 4
Tissue specific RNAi against vang-1.

In C. elegans and mice, VANG-1 and Vangl2Lp have been connected with correct uterine epithelium development in the reproductive tract [12], [66], but its function in meiotic maturation and ovulation is still ellusive. Both processes are regulated by intense signaling between the germline and the proximal gonadal sheath cells, specialized myo-epithelial cells that surround and form gap junctions with oocytes [67][70]. During ovulation, sheath cells contract rapidly, the distal constriction of the spermatheca dilates, and sheath cells pull the distal spermatheca over the mature oocyte [71]. The decreased fertility/brood size, ovulation rate, and the increased reproduction span of tm1422 animals (Fig. 2 A,D–E) suggests VANG-1 being involved in the communication between germline and somatic gonad. To test if vang-1 also acts in the germline to control life span by insulin/IGF-1-like signaling, we performed germline-specific RNAi [62]. vang-1(RNAi) in rrf-1 led to a significant increase in life span (13.5%, Fig. 4B; Table 1), which is about two third of whole life span extension observed in vang-1(RNAi) animals (Fig. 1A; Table 1). In contrast, depletion of VANG-1 in the enhanced-neuronal RNAi strains TU3311 and TU3401 [64], has no effect on C. elegans life span extension (Fig. 4C; Table 1). As suggested by Calixto et al. [64] the neuronal expression of sid-1 in TU3311 might serve as a sink for double-stranded RNA used by non-neuronal RNAi and thus could explain why vang-1(RNAi) in TU3311 leads not to the same life span extension as in WT. Additionally, vang-1(tm1422) individuals have an intact chemosensory apparatus and are “open” to the environment (personal comunication with N.J. Storm - it has been testet two times with up to 30 individuals per experiment for uptake of DiI [72]). Dye-fill defective (dyf-phenotype) mutants have previous been found long-lived [73]. Taken together, our findings of tissue-specific RNAi against vang-1 in combination with in-situ hybridization data of vang-1, daf-2 and daf-16 (Fig. S2) implicate the germline to be the primary site of vang-1 action concerning longevity in C. elegans. Components of the insulin/IGF-1-like signaling pathway have already been implicated to act in the germline, e.g., Michaelson et al. found that the effect of reducing daf-2 signaling on larval germline proliferation is dependent on daf-16 [74].

In summary, we have identified a link between the C. elegans planar cell polarity key player vang-1 and insulin/IGF-1-dependent extension of life span. Mutations in vang-1 show the typical phenotype of age-mutants, including longevity, slow growth, multiple stress resistances, reduced lipofuscin accumulation, and reduced brood size. The germline, but not the intestine or neurons seems to be the primary site of vang-1 function, which may operate in the same pathway as daf-2 and daf-16 to extend life span of about 40% in C. elegans.

Methods

C. elegans strains and alleles

Maintenance and handling of C. elegans were carried out as described previously [75]. Bristol N2 was used as the WT strain. WT or mutant worms were synchronized as described previously [76].

Single mutants were as follows

TM1422: vang-1(tm1422)X (outcrossed ×3); RB1125: vang-1(ok1142) X; CB1370: daf-2(e1370) III; CF1038: daf-16(mu86) I; NL2098: rrf-1(pk1417) I.

Transgenic strains were as follows

OLB11: rde-1(ne219);[pOLB11(elt-2p::rde-1)+pRF4(rol-6(su1006))]; TU3311: [unc-119p::YFP+unc-119p::sid-1]; TU3401: sid-1(pk3321) V; [pCFJ90(myo-2p::mCherry)+unc-119p::sid-1]; TJ356: integrated DAF-16::GFP roller strain [50] (for further details see: https://cgcdb.msi.umn.edu/strain.php?id=13306).

RNA-mediated interference (RNAi)

RNAi by “feeding” was performed essentially as described by others [77]. In brief, after amplification of a single colony overnight (37°C, LBamp tet medium), HT115(DE3) bacteria (RNase III-deficient E. coli strain, carrying IPTG-inducible T7-polymerase) [77], [78] were diluted to an OD600 of 0.9, and after addition of IPTG (1 mM) seeded on NGMamp tet plates (containing 1 mM IPTG). Bacteria were further incubated overnight at room temperature (~22°C) to allow the expression of double-stranded RNA. HT115(DE3) bacteria harboring the empty KS+ based vector L4440 (containing two T7 promoters flanking a polylinker) were used as a control for RNAi “feeding” experiments. RNAi clones against vang-1 and daf-16 were obtained from the Ahringer RNAi “feeding”-library (Geneservice Limited, Cambridge, UK) while daf-2 “feeding”-clone was kindly provided by Dr. Andrew Dillin [79] (see supporting information S2 for sequencing results of RNAi “feeding”-clones).

Life span assay

Life span was determined at 25°C, if not stated otherwise. Because vang-1(tm1422) shows a delayed egg laying phenotype, synchronization was performed as follows: embryos were randomly collected from cut-off worms, transferred and grown on plates (three plates per trial) either seeded with OP50 [75] or HT115(DE3) [77], [78] bacteria harboring the empty L4440 “feeding”-vector or L4440 with a fragment of the gene of interest [77]. Worms were transferred to fresh plates every day during time of reproduction but at least every third day. Individuals were considered as dead when stopped moving and not responded to gentle touches. When dying upon “rupture”/“bag of worms” phenotypes or disappearance occurred, the animal was censored on that day. The resulting data sets were analyzed using Kaplan-Meier survival test and weighted log-rank tests [80].

Determination of progeny

C. elegans populations were synchronized and hatched on NGM Agar plates at 25°C. On day three, single worms were transferred as L4 larvae to 35 mm NGM-plates with NGM agar. Adult worms were transferred to fresh plates and then their progenies were counted each day. The experiment was stopped when production of progeny ceased.

Dauer assay

The assay was performed as described elsewhere [53]. In brief, some gravid adults were put on individual tagged 60 mm NGM-plates where they laid eggs for 4–6 h at 20°C before they were removed again. Plates were shifted to the assay temperature of 27°C. After 60 h the stages were scored for L1 diapause and dauers. Farther grown worms (individuals larger than L2 larvae but not predauer/dauer stages) were pooled as “other”.

Reproductive span of self-fertile animals (modified after [46])

Ten hermaphrodites per trial were individually transferred to fresh 35 mm NGM-plates seeded with OP50 daily. No production of progeny for 48 h marked reproductive cessation. Individuals were censored if they died or matricide occurred. All trials were conducted at 20°C with age synchronized populations. Unpaired t-test was used to test null hypothesis.

Determination of ovulation rate (modified after [71])

Documentation of ovulation rates were performed using a Zeiss Axioplan 2 microscope. Age-synchronized worms with more than six oocytes in-utero were transferred to small agarose pads on a microscope slide and coated with a cover slip. The number of ovulated oocytes per animal was counted for 3 h and slides were kept in a moisture chamber at room temperature.

DAF-16::GFP translocation

Synchronized populations of TJ356 (DAF-16::GFP) [50] worms were kept for 72 h at 25°C on NGM plates seeded with RNAi HT115 bacteria either carrying the empty “feeding”-vector or a fragment of vang-1 cDNA. 15 Individuals per trial were transferred to small agarose pads (3%) on a microscope slide, anesthetized with levamisole (1%), coated with a cover slip, illuminated with UV light under an Axiolab fluorescence microscope (Zeiss, Göttingen, Germany) and dedicated to three categories concerning DAF-16::GFP translocation: “cytoplasmatic” (uniform distribution of DAF-16::GFP), “intermediate” (clearly distinguishable DAF-16::GFP in some nuclei), and “nuclear” (DAF-16::GFP in nearly all nuclei with low background fluorescence).

Lipofuscin accumulation

WT C. elegans were synchronized, hatched on NGM Agar plates at 20°C and transferred to fresh plates every second day. At day five and day ten, individuals were placed on microscope slides, anaesthetized with 20 mM sodium azide in M9 buffer [76] and coated with a cover slip. Epifluorescence (excitation, 365 nm; emission, 420 nm) images were taken with Image ProPlus software (Version 4.5, MediaCybernetics, Silver Spring, MD, USA) using a CoolSnap CF Digital Monochrome Camera (Intas, Göttingen, Germany) mounted on an Axiolab fluorescence microscope (Zeiss, Göttingen, Germany) and using a 100× oil immersion objective. The fluorescence intensity of individual worms was determined densitometrically as relative fluorescence units (RFU: ODindividual−ODbackground/mm2).

Assessment of resistance to thermal/oxidative stress and determination of intracellular ROS accumulation in C. elegans

The resistance of WT and mutant animals to thermal stress was assessed by a semi-automated assay according to [81] with some modifications described in [82]. After synchronization [76] both strains were cultured on NGM plates with OP50 bacteria [75] for five days at 20°C. Worms were then washed in PBST (PBS/0.1% Tween 20) and individually transferred with 1 µl PBST to the wells of a 384-well microtiter plate (Greiner Bio-One, Frickenhausen, Germany, #788096) containing 9 µl PBST with 1×107 OP50 bacteria/ml [82]. Immediately after transfer 10 µl of 2 µM SYTOX® Green nucleic acid stain (Molecular Probes Inc., Leiden, Netherlands) in PBS was added to the wells and the plate was sealed using BackSeal-96/384 Black (Perkin Elmer, Wellesley, USA, #6005189) to avoid evaporation. SYTOX® Green can only enter cells with compromised plasma membranes and exerts a bright fluorescence in the DNA-bound state. Therefore, the fluorescence intensity is an indicator for cellular damage and hence for the viability of worms [81]. For the application of thermal stress the fluorescence reader (Wallace Victor2 1420 multilabel counter, Perkin Elmer, Wellesley, USA) was preheated to 37°C. The measurement of each well through the transparent bottom of the microtiter plate (excitation, 485 nm; emission, 535 nm) was carried out for a minimum of 13 h with intervals of 15 min and a 0.2 s integration time. Fluorescence curves for every single well were obtained and individual cut off values were determined by multiplying the background fluorescence (average of the first four measurement readings) by a factor of three [81][83]. The time point when fluorescence exceeded the cut off value was defined as the point of death of the corresponding worm and the survival curves as well as the mean life spans were assessed from these individual times of death.

To compare resistance to oxidative stress WT and mutant animals synchronized [76] and L4 larvae were incubated for approximate 5 h at 20°C in liquid NGM containing 200 µM juglone, a redox cycler that generates intracellular oxidative stress [84]. Worms were then allowed to regenerate on NGM plates with OP50 bacteria [75] for about 20 h at 20°C before viability was determined by touch provoked movement [85].

For the determination of the intracellular amount of ROS synchronized WT and mutant larvae were cultured as described above and individually transferred with 1 µl PBST to the wells of a 384-well microtiter plate containing 7 µl PBS [86]. After the complete transfer of the individual worms 2 µl 250 µM 2,7-dichlorodihydrofluorescein diacetate (H2DCF-DA; Molecular Probes Inc., Leiden, Netherlands) in PBS (final concentration, 50 µM) was added to the wells and the plate was sealed (see above). After entering cells H2DCF-DA is intracellular converted to membrane-impermeable, non-fluorescent H2DCF, which then can be oxidized by ROS to yield fluorescent DCF and thus is a marker for the individual amount of intracellular ROS in a single worm [82], [83], [86]. The fluorescence of each well is then measured through the transparent bottom in a fluorescence reader (see above) every 15 min for a minimum of 13 h at 37°C (1.0 s integration time; excitation, 485 nm; emission, 535 nm).

Supporting Information

Figure S1

DAF-16::GFP translocation into the nucleus. In TJ356 (DAF-16::GFP) worms [50], RNAi against vang-1 at room temperature (RT) led to 12% and 4% intermediate and nuclear localization of DAF-16::GFP, respectively (n = 49*). In contrast, TJ356 control animals fed with RNAi HT115 bacteria, carrying the empty “feeding”-vector, showed 100% cytoplasmic localization of DAF-16::GFP (n = 70*). Under heat stress condition (27°C), vang-1(RNAi) causes 45% and 12% intermediate and nuclear localization of DAF-16::GFP, respectively (n = 42*). In comparison, TJ356 control animals showed 42% intermediate- and 3% nuclear localization of DAF-16::GFP (n = 95*). *(p<0.05 by two-way ANOVA with Bonferroni's post hoc test; three or more independent trials).

(TIF)

Figure S2

Expression patterns in C. elegans adults of daf-2 (A), daf-16 (B) and vang-1 (C) genes. All images represent in situ hybridization to endogenous transcripts (enriched in the gonad, arrows) and are taken from the Nematode Expression Data Base (http://nematode.lab.nig.ac.jp/db2/index.php). Scale bars: 60 µm.

(TIF)

Supporting Information S1

Sequences of VANG-1, VANG-1tm1422 and VANG-1ok1142 proteins. Missing amino acids in tm1422 and ok1142 are shown in red and blue, respectively. Additional amino acids in ok1142 are shown in yellow. For further details concerning VANG-1 see [13].

(DOCX)

Supporting Information S2

Sequences of RNAi “feeding”-clones.

(DOCX)

Acknowledgments

The authors would like to thank: C. Cowan, A. Wodarz, A. Müller and J. Nelson for critical reading of the manuscript, N.J. Storm for sharing data, A. Dillin for providing daf-2 RNAi “feeding”-clone, the Mitani lab and the C. elegans Knockout Consortium for providing tm1422 and ok1142, respectively. Some nematode strains used in this work were provided by the Caenorhabditis Genetics Center, which is funded by the NIH National Center for Research Resources (NCRR).

Footnotes

Competing Interests: Andreas Kampkötter is employed by Bayer Animal Health GmbH. However, this does not alter the authors' adherence to all the PLoS ONE policies on sharing data and materials.

Funding: Deutsche Forschungsgemeinschaft (SFB590 TP B3) to OB. SH is a fellow of the Jürgen Manchot Stiftung. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.

References

1. Nelson WJ, Nusse R. Convergence of Wnt, beta-catenin, and cadherin pathways. Science. 2004;303:1483–1487. [PMC free article] [PubMed]
2. Cadigan K, Nusse R. Wnt signaling: a common theme in animal development. Genes And Development. 1997;11:3286–3305. [PubMed]
3. Wodarz A, Nusse R. Mechanisms of Wnt signaling in development. Annu Rev Cell Dev Biol. 1998;14:59–88. [PubMed]
4. Wang Y, Nathans J. Tissue/planar cell polarity in vertebrates: new insights and new questions. Development. 2007;134:647–658. [PubMed]
5. Wu J, Mlodzik M. A quest for the mechanism regulating global planar cell polarity of tissues. Trends Cell Biol. 2009;19:295–305. [PMC free article] [PubMed]
6. Axelrod JD. Progress and challenges in understanding planar cell polarity signaling. Semin Cell Dev Biol. 2009;20:964–971. [PubMed]
7. Goodrich LV, Strutt D. Principles of planar polarity in animal development. Development. 2011;138:1877–1892. [PMC free article] [PubMed]
8. Jenny A. Planar cell polarity signaling in the Drosophila eye. Current topics in developmental biology. 2010;93:189–227. [PubMed]
9. Keller R. Shaping the vertebrate body plan by polarized embryonic cell movements. Science. 2002;298:1950–1954. [PubMed]
10. Keller R, Shook D. Dynamic determinations: patterning the cell behaviours that close the amphibian blastopore. Philos Trans R Soc Lond B Biol Sci. 2008;363:1317–1332. [PMC free article] [PubMed]
11. Mirkovic I, Gault WJ, Rahnama M, Jenny A, Gaengel K, et al. Nemo kinase phosphorylates beta-catenin to promote ommatidial rotation and connects core PCP factors to E-cadherin-beta-catenin. Nature structural & molecular biology 2011 [PMC free article] [PubMed]
12. Green J, Inoue T, Sternberg P. Opposing Wnt pathways orient cell polarity during organogenesis. Cell. 2008;134:646–656. [PMC free article] [PubMed]
13. Hoffmann M, Segbert C, Helbig G, Bossinger O. Intestinal tube formation in Caenorhabditis elegans requires vang-1 and egl-15 signaling. Dev Biol. 2010;339:268–279. [PubMed]
14. Wu M, Herman MA. A novel noncanonical Wnt pathway is involved in the regulation of the asymmetric B cell division in C. elegans. Dev Biol. 2006;293:316–329. [PubMed]
15. Leung B, Hermann GJ, Priess JR. Organogenesis of the Caenorhabditis elegans intestine. Dev Biol. 1999;216:114–134. [PubMed]
16. Johnson TE, Henderson S, Murakami S, de Castro E, de Castro SH, et al. Longevity genes in the nematode Caenorhabditis elegans also mediate increased resistance to stress and prevent disease. J Inherit Metab Dis. 2002;25:197–206. [PubMed]
17. Kenyon CJ. The genetics of ageing. Nature. 2010;464:504–512. [PubMed]
18. Antebi A. Genetics of aging in Caenorhabditis elegans. PLoS Genet. 2007;3:1565–1571. [PMC free article] [PubMed]
19. Wolff S, Dillin A. The trifecta of aging in Caenorhabditis elegans. Exp Gerontol. 2006;41:894–903. [PubMed]
20. Friedman D, Johnson T. A mutation in the age-1 gene in Caenorhabditis elegans lengthens life and reduces hermaphrodite fertility. Genetics. 1988;118:75–86. [PMC free article] [PubMed]
21. Morris J, Tissenbaum H, Ruvkun G. A phosphatidylinositol-3-OH kinase family member regulating longevity and diapause in Caenorhabditis elegans. Nature. 1996;382:536–539. [PubMed]
22. Wolkow CA, Munoz MJ, Riddle DL, Ruvkun G. Insulin receptor substrate and p55 orthologous adaptor proteins function in the Caenorhabditis elegans daf-2/insulin-like signaling pathway. J Biol Chem. 2002;277:49591–49597. [PubMed]
23. Hertweck M, Göbel C, Baumeister R. C. elegans SGK-1 is the critical component in the Akt/PKB kinase complex to control stress response and life span. Dev Cell. 2004;6:577–588. [PubMed]
24. Paradis S, Ailion M, Toker AS, Thomas JH, Ruvkun G. A PDK1 homolog is necessary and sufficient to transduce AGE-1 PI3 kinase signals that regulate diapause in Caenorhabditis elegans. Genes Dev. 1999;13:1438–1452. [PMC free article] [PubMed]
25. Paradis S, Ruvkun G. Caenorhabditis elegans Akt/PKB transduces insulin receptor-like signals from AGE-1 PI3 kinase to the DAF-16 transcription factor. Genes Dev. 1998;12:2488–2498. [PMC free article] [PubMed]
26. Ogg S, Paradis S, Gottlieb S, Patterson GI, Lee L, et al. The Fork head transcription factor DAF-16 transduces insulin-like metabolic and longevity signals in C. elegans. Nature. 1997;389:994–999. [PubMed]
27. Lin KT, Dorman J, Rodan A, Kenyon C. daf-16: An HNF-3/forkhead family member that can function to double the life-span of Caenorhabditis elegans. Science. 1997;278:1319–1322. [PubMed]
28. Huang H, Tindall DJ. Dynamic FoxO transcription factors. J Cell Sci. 2007;120:2479–2487. [PubMed]
29. Kenyon C, Chang JW, Gensch E, Rudner A, Tabtiang R. A C. elegans mutant that lives twice as long as wild type. Nature. 1993;366:461–464. [PubMed]
30. An JH, Blackwell TK. SKN-1 links C. elegans mesendodermal specification to a conserved oxidative stress response. Genes & development. 2003;17:1882–1893. [PMC free article] [PubMed]
31. Hsu AL, Murphy CT, Kenyon C. Regulation of aging and age-related disease by DAF-16 and heat-shock factor. Science. 2003;300:1142–1145. [PubMed]
32. Darken RS, Scola AM, Rakeman AS, Das G, Mlodzik M, et al. The planar polarity gene strabismus regulates convergent extension movements in Xenopus. Embo J. 2002;21:976–985. [PMC free article] [PubMed]
33. Goto T, Keller R. The planar cell polarity gene strabismus regulates convergence and extension and neural fold closure in Xenopus. Dev Biol. 2002;247:165–181. [PubMed]
34. Kibar Z, Vogan KJ, Groulx N, Justice MJ, Underhill DA, et al. Ltap, a mammalian homolog of Drosophila Strabismus/Van Gogh, is altered in the mouse neural tube mutant Loop-tail. Nat Genet. 2001;28:251–255. [PubMed]
35. Luscombe NM, Qian J, Zhang Z, Johnson T, Gerstein M. The dominance of the population by a selected few: power-law behaviour applies to a wide variety of genomic properties. Genome Biol. 2002;3:RESEARCH0040. [PMC free article] [PubMed]
36. Guo S, Kemphues KJ. A non-muscle myosin required for embryonic polarity in Caenorhabditis elegans. Nature. 1996;382:455–458. [PubMed]
37. Harman D. Aging: a theory based on free radical and radiation chemistry. J Gerontol. 1956;11:298–300. [PubMed]
38. Braeckman B, Houthoofd K, Vanfleteren J. Assessing metabolic activity in aging Caenorhabditis elegans: concepts and controversies. Aging Cell. 2002;1:82–88; discussion 102–103. [PubMed]
39. Finkel T, Holbrook NJ. Oxidants, oxidative stress and the biology of ageing. Nature. 2000;408:239–247. [PubMed]
40. Van Raamsdonk JM, Hekimi S. Reactive Oxygen Species and Aging in Caenorhabditis elegans: Causal or Casual Relationship? Antioxid Redox Signal. 2010;13:1911–1953. [PubMed]
41. Hekimi S, Lapointe J, Wen Y. Taking a “good” look at free radicals in the aging process. Trends in Cell Biology 2011 [PubMed]
42. Garigan D, Hsu AL, Fraser AG, Kamath RS, Ahringer J, et al. Genetic analysis of tissue aging in Caenorhabditis elegans: a role for heat-shock factor and bacterial proliferation. Genetics. 2002;161:1101–1112. [PMC free article] [PubMed]
43. Gerstbrein B, Stamatas G, Kollias N, Driscoll M. In vivo spectrofluorimetry reveals endogenous biomarkers that report healthspan and dietary restriction in Caenorhabditis elegans. Aging Cell. 2005;4:127–137. [PubMed]
44. Hughes SC, Evason K, Xiong C, Kornfeld K. Genetic and pharmacological factors that influence reproductive aging in nematodes. PLoS Genet. 2007;3:e25. [PMC free article] [PubMed]
45. Andux S, Ellis RE. Apoptosis maintains oocyte quality in aging Caenorhabditis elegans females. PLoS Genet. 2008;4:e1000295. [PMC free article] [PubMed]
46. Luo S, Shaw WM, Ashraf J, Murphy CT. TGF-beta Sma/Mab signaling mutations uncouple reproductive aging from somatic aging. PLoS Genet. 2009;5:e1000789. [PMC free article] [PubMed]
47. Kenyon C. The plasticity of aging: insights from long-lived mutants. Cell. 2005;120:449–460. [PubMed]
48. Lin KT, Hsin H, Libina N, Kenyon C. Regulation of the Caenorhabditis elegans longevity protein DAF-16 by insulin/IGF-1 and germline signaling. Nat Genet. 2001;28:139–145. [PubMed]
49. Samuelson AV, Carr CE, Ruvkun G. Gene activities that mediate increased life span of C. elegans insulin-like signaling mutants. Genes Dev. 2007;21:2976–2994. [PMC free article] [PubMed]
50. Henderson S, Johnson TE. daf-16 integrates developmental and environmental inputs to mediate aging in the nematode Caenorhabditis elegans. Curr Biol. 2001;11:1975–1980. [PubMed]
51. Baugh LR, Sternberg PW. DAF-16/FOXO regulates transcription of cki-1/Cip/Kip and repression of lin-4 during C. elegans L1 arrest. Curr Biol. 2006;16:780–785. [PubMed]
52. Gerisch B, Weitzel C, Kober-Eisermann C, Rottiers V, Antebi A. A hormonal signaling pathway influencing C. elegans metabolism, reproductive development, and life span. Dev Cell. 2001;1:841–851. [PubMed]
53. Hu PJ, Xu J, Ruvkun G. Two membrane-associated tyrosine phosphatase homologs potentiate C. elegans AKT-1/PKB signaling. PLoS Genet. 2006;2:e99. [PMC free article] [PubMed]
54. Gottlieb S, Ruvkun G. daf-2, daf-16 and daf-23: genetically interacting genes controlling Dauer formation in Caenorhabditis elegans. Genetics. 1994;137:107–120. [PMC free article] [PubMed]
55. Landis JN, Murphy CT. Integration of diverse inputs in the regulation of Caenorhabditis elegans DAF-16/FOXO. Dev Dyn 2010 [PMC free article] [PubMed]
56. Berman JR, Kenyon C. Germ-cell loss extends C. elegans life span through regulation of DAF-16 by kri-1 and lipophilic-hormone signaling. Cell. 2006;124:1055–1068. [PubMed]
57. Hsin H, Kenyon C. Signals from the reproductive system regulate the lifespan of C. elegans. Nature. 1999;399:362–366. [PubMed]
58. Tullet JM, Hertweck M, An JH, Baker J, Hwang JY, et al. Direct inhibition of the longevity-promoting factor SKN-1 by insulin-like signaling in C. elegans. Cell. 2008;132:1025–1038. [PMC free article] [PubMed]
59. Okuyama T, Inoue H, Ookuma S, Satoh T, Kano K, et al. The ERK MAPK pathway regulates longevity through SKN-1 and insulin-like signaling in C. elegans. J Biol Chem 2010 [PMC free article] [PubMed]
60. Pilipiuk J, Lefebvre C, Wiesenfahrt T, Legouis R, Bossinger O. Increased IP3/Ca2+ signaling compensates depletion of LET-413/DLG-1 in C. elegans epithelial junction assembly. Dev Biol. 2009;327:34–47. [PubMed]
61. McGhee JD, Fukushige T, Krause MW, Minnema SE, Goszczynski B, et al. ELT-2 is the predominant transcription factor controlling differentiation and function of the C. elegans intestine, from embryo to adult. Dev Biol. 2009;327:551–565. [PMC free article] [PubMed]
62. Sijen T, Fleenor J, Simmer F, Thijssen KL, Parrish S, et al. On the role of RNA amplification in dsRNA-triggered gene silencing. Cell. 2001;107:465–476. [PubMed]
63. Sanchez-Alvarez L, Visanuvimol J, McEwan A, Su A, Imai JH, et al. VANG-1 and PRKL-1 Cooperate to Negatively Regulate Neurite Formation in Caenorhabditis elegans. PLoS genetics. 2011;7:e1002257. [PMC free article] [PubMed]
64. Calixto A, Chelur D, Topalidou I, Chen X, Chalfie M. Enhanced neuronal RNAi in C. elegans using SID-1. Nature methods. 2010;7:554–559. [PMC free article] [PubMed]
65. Libina N, Berman JR, Kenyon C. Tissue-specific activities of C. elegans DAF-16 in the regulation of lifespan. Cell. 2003;115:489–502. [PubMed]
66. vandenBerg AL, Sassoon DA. Non-canonical Wnt signaling regulates cell polarity in female reproductive tract development via van gogh-like 2. Development. 2009;136:1559–1570. [PMC free article] [PubMed]
67. Greenstein D, Hird S, Plasterk RH, Andachi Y, Kohara Y, et al. Targeted mutations in the Caenorhabditis elegans POU homeo box gene ceh-18 cause defects in oocyte cell cycle arrest, gonad migration, and epidermal differentiation. Genes Dev. 1994;8:1935–1948. [PubMed]
68. McCarter J, Bartlett B, Dang T, Schedl T. Soma-germ cell interactions in Caenorhabditis elegans: multiple events of hermaphrodite germline development require the somatic sheath and spermathecal lineages. Dev Biol. 1997;181:121–143. [PubMed]
69. Miller MA, Ruest PJ, Kosinski M, Hanks SK, Greenstein D. An Eph receptor sperm-sensing control mechanism for oocyte meiotic maturation in Caenorhabditis elegans. Genes Dev. 2003;17:187–200. [PMC free article] [PubMed]
70. Rose K, Winfrey V, Hoffman L, Hall D, Furuta T, et al. The POU gene ceh-18 promotes gonadal sheath cell differentiation and function required for meiotic maturation and ovulation in Caenorhabditis elegans. Dev Biol. 1997;192:59–77. [PubMed]
71. McCarter J, Bartlett B, Dang T, Schedl T. On the control of oocyte meiotic maturation and ovulation in Caenorhabditis elegans. Dev Biol. 1999;205:111–128. [PubMed]
72. Mukhopadhyay A, Deplancke B, Walhout AJ, Tissenbaum HA. C. elegans tubby regulates life span and fat storage by two independent mechanisms. Cell Metab. 2005;2:35–42. [PubMed]
73. Apfeld J, Kenyon C. Regulation of lifespan by sensory perception in Caenorhabditis elegans. Nature. 1999;402:804–809. [PubMed]
74. Michaelson D, Korta DZ, Capua Y, Hubbard EJ. Insulin signaling promotes germline proliferation in C. elegans. Development. 2010;137:671–680. [PMC free article] [PubMed]
75. Brenner S. The genetics of Caenorhabditis elegans. Genetics. 1974;77:71–94. [PMC free article] [PubMed]
76. Lewis JA, Fleming JT. Basic culture methods. Methods Cell Biol. 1995;48:3–29. [PubMed]
77. Kamath RS, Martinez-Campos M, Zipperlen P, Fraser AG, Ahringer J. Effectiveness of specific RNA-mediated interference through ingested double-stranded RNA in Caenorhabditis elegans. Genome Biol. 2001;2:research 0002.0001–0002.0010. [PMC free article] [PubMed]
78. Timmons L, Court DL, Fire A. Ingestion of bacterially expressed dsRNAs can produce specific and potent genetic interference in Caenorhabditis elegans. Gene. 2001;263:103–112. [PubMed]
79. Cohen E, Bieschke J, Perciavalle RM, Kelly JW, Dillin A. Opposing activities protect against age-onset proteotoxicity. Science. 2006;313:1604–1610. [PubMed]
80. Woolson RF, Clarke WR. Statistical Methods for the Analysis of Biomedical Data. New York: Wiley; 2002.
81. Gill MS, Olsen A, Sampayo JN, Lithgow GJ. An automated high-throughput assay for survival of the nematode Caenorhabditis elegans. Free Radic Biol Med. 2003;35:558–565. [PubMed]
82. Kampkötter A, Nkwonkam CG, Zurawski RF, Timpel C, Chovolou Y, et al. Investigations of protective effects of the flavonoids quercetin and rutin on stress resistance in the model organism Caenorhabditis elegans. Toxicology. 2007;234:113–123. [PubMed]
83. Kampkötter A, Pielarski T, Rohrig R, Timpel C, Chovolou Y, et al. The Ginkgo biloba extract EGb761 reduces stress sensitivity, ROS accumulation and expression of catalase and glutathione S-transferase 4 in Caenorhabditis elegans. Pharmacol Res. 2007;55:139–147. [PubMed]
84. de Castro E, Hegi de Castro S, Johnson TE. Isolation of long-lived mutants in Caenorhabditis elegans using selection for resistance to juglone. Free Radic Biol Med. 2004;37:139–145. [PubMed]
85. Lithgow GJ, White TM, Melov S, Johnson TE. Thermotolerance and extended life-span conferred by single-gene mutations and induced by thermal stress. Proc Natl Acad Sci USA. 1995;92:7540–7544. [PMC free article] [PubMed]
86. Kampkötter A, Gombitang Nkwonkam C, Zurawski RF, Timpel C, Chovolou Y, et al. Effects of the flavonoids kaempferol and fisetin on thermotolerance, oxidative stress and FoxO transcription factor DAF-16 in the model organism Caenorhabditis elegans. Arch Toxicol. 2007;81:849–858. [PubMed]

Articles from PLoS ONE are provided here courtesy of Public Library of Science

Formats:

Related citations in PubMed

See reviews...See all...

Cited by other articles in PMC

See all...

Links

Recent Activity

Your browsing activity is empty.

Activity recording is turned off.

Turn recording back on

See more...