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Nucleic Acids Res. 1979; 6(5): 1805–1816.
PMCID: PMC327812

Nucleosome cores reconstituted from poly (dA-dT) and the octamer of histones.

Abstract

In this paper we describe a detailed investigation of the reconstitution of nucleosome cores from poly (dA-dT) and the octamer of histones. We also attempted the reconstitution from the copolymers poly dA.poly dT, poly dG.poly dC and poly (dG-dC). The repeat of the reconstituted chromatin fibre is discussed. The micrococcal nuclease released poly (dA-dT) core particle is found to contain a considerably narrower DNA size distribution that of the native random DNA nucleosome core (12). In addition we have succeeded in obtaining small crystals of the poly (dA-dT) nucleosome core. The DNAase I digestion pattern of the poly (dA-dT) containing nucleosome core is presented. The periodicity of DNAase I cutting sites is found to be about 10.5 bases and is similar to that of the native nucleosome core (12, 13).

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Selected References

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  • Kornberg RD. Structure of chromatin. Annu Rev Biochem. 1977;46:931–954. [PubMed]
  • Lutter LC. Kinetic analysis of deoxyribonuclease I cleavages in the nucleosome core: evidence for a DNA superhelix. J Mol Biol. 1978 Sep 15;124(2):391–420. [PubMed]
  • Spadafora C, Oudet P, Chambon P. The same amount of DNA is organized in in vitro-assembled nucleosomes irrespective of the origin of the histones. Nucleic Acids Res. 1978 Oct;5(10):3479–3489. [PMC free article] [PubMed]
  • Thomas JO, Butler PJ. The nucleosome core protein. Cold Spring Harb Symp Quant Biol. 1978;42(Pt 1):119–125. [PubMed]
  • Steinmetz M, Streeck RE, Zachau HG. Closely spaced nucleosome cores in reconstituted histone.DNA complexes and histone-H1-depleted chromatin. Eur J Biochem. 1978 Feb;83(2):615–628. [PubMed]
  • Oudet P, Gross-Bellard M, Chambon P. Electron microscopic and biochemical evidence that chromatin structure is a repeating unit. Cell. 1975 Apr;4(4):281–300. [PubMed]
  • Axel R, Melchior W, Jr, Sollner-Webb B, Felsenfeld G. Specific sites of interaction between histones and DNA in chromatin. Proc Natl Acad Sci U S A. 1974 Oct;71(10):4101–4105. [PMC free article] [PubMed]
  • Laskey RA, Mills AD, Morris NR. Assembly of SV40 chromatin in a cell-free system from Xenopus eggs. Cell. 1977 Feb;10(2):237–243. [PubMed]
  • Finch JT, Lutter LC, Rhodes D, Brown RS, Rushton B, Levitt M, Klug A. Structure of nucleosome core particles of chromatin. Nature. 1977 Sep 1;269(5623):29–36. [PubMed]
  • Lutter LC. Precise location of DNase I cutting sites in the nucleosome core determined by high resolution gel electrophoresis. Nucleic Acids Res. 1979 Jan;6(1):41–56. [PMC free article] [PubMed]
  • Thomas JO, Butler PJ. Characterization of the octamer of histones free in solution. J Mol Biol. 1977 Nov;116(4):769–781. [PubMed]
  • Thomas JO, Kornberg RD. An octamer of histones in chromatin and free in solution. Proc Natl Acad Sci U S A. 1975 Jul;72(7):2626–2630. [PMC free article] [PubMed]
  • Finch JT, Noll M, Kornberg RD. Electron microscopy of defined lengths of chromatin. Proc Natl Acad Sci U S A. 1975 Sep;72(9):3320–3322. [PMC free article] [PubMed]
  • Maniatis T, Jeffrey A, van deSande H. Chain length determination of small double- and single-stranded DNA molecules by polyacrylamide gel electrophoresis. Biochemistry. 1975 Aug 26;14(17):3787–3794. [PubMed]
  • Peacock AC, Dingman CW. Resolution of multiple ribonucleic acid species by polyacrylamide gel electrophoresis. Biochemistry. 1967 Jun;6(6):1818–1827. [PubMed]
  • Arnott S, Selsing E. Letter: The structure of polydeoxyguanylic acid with polydeoxycytidylic acid. J Mol Biol. 1974 Sep 15;88(2):551–552. [PubMed]
  • Arnott S, Selsing E. Structures for the polynucleotide complexes poly(dA) with poly (dT) and poly(dT) with poly(dA) with poly (dT). J Mol Biol. 1974 Sep 15;88(2):509–521. [PubMed]
  • Wells RD, Blakesley RW, Hardies SC, Horn GT, Larson JE, Selsing E, Burd JF, Chan HW, Dodgson JB, Jensen KF, et al. The role of DNA structure in genetic regulation. CRC Crit Rev Biochem. 1977;4(3):305–340. [PubMed]
  • Sollner-Webb B, Melchior W, Jr, Felsenfeld G. DNAase I, DNAase II and staphylococcal nuclease cut at different, yet symmetrically located, sites in the nucleosome core. Cell. 1978 Jul;14(3):611–627. [PubMed]
  • Scheffler IE, Elson EL, Baldwin RL. Helix formation by dAT oligomers. I. Hairpin and straight-chain helices. J Mol Biol. 1968 Sep 28;36(3):291–304. [PubMed]
  • Noll M. Internal structure of the chromatin subunit. Nucleic Acids Res. 1974 Nov;1(11):1573–1578. [PMC free article] [PubMed]
  • Ehrlich SD, Bertazzoni U, Bernardi G. The specificity of pancreatic deoxyribonuclease. Eur J Biochem. 1973 Dec 3;40(1):143–147. [PubMed]
  • Noll M, Kornberg RD. Action of micrococcal nuclease on chromatin and the location of histone H1. J Mol Biol. 1977 Jan 25;109(3):393–404. [PubMed]

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