• We are sorry, but NCBI web applications do not support your browser and may not function properly. More information
Logo of narLink to Publisher's site
Nucleic Acids Res. Oct 10, 1981; 9(19): 5109–5124.
PMCID: PMC327502

Secondary structure formation during RNA synthesis.

Abstract

We observed the secondary structures that formed in an RNA molecule during its synthesis. Some of the secondary structures seen in nascent chains were observed to form, then to dissociate in favor of an alternative structure, and then to reform, as chain growth continued. The results show that secondary structures in an RNA molecule are in a state of dynamic equilibrium, and that the extension of a sequence by chain growth, or the reduction of a sequence by processing, may result in significant changes in the secondary structures that are present.

Full text

Full text is available as a scanned copy of the original print version. Get a printable copy (PDF file) of the complete article (1.9M), or click on a page image below to browse page by page. Links to PubMed are also available for Selected References.

Images in this article

Click on the image to see a larger version.

Selected References

These references are in PubMed. This may not be the complete list of references from this article.
  • Mills DR, Dobkin C, Kramer FR. Template-determined, variable rate of RNA chain elongation. Cell. 1978 Oct;15(2):541–550. [PubMed]
  • Haruna I, Spiegelman S. Specific template requirments of RNA replicases. Proc Natl Acad Sci U S A. 1965 Aug;54(2):579–587. [PMC free article] [PubMed]
  • Kacian DL, Mills DR, Kramer FR, Spiegelman S. A replicating RNA molecule suitable for a detailed analysis of extracellular evolution and replication. Proc Natl Acad Sci U S A. 1972 Oct;69(10):3038–3042. [PMC free article] [PubMed]
  • Mills DR, Kramer FR, Spiegelman S. Complete nucleotide sequence of a replicating RNA molecule. Science. 1973 Jun 1;180(4089):916–927. [PubMed]
  • Kramer FR, Mills DR. RNA sequencing with radioactive chain-terminating ribonucleotides. Proc Natl Acad Sci U S A. 1978 Nov;75(11):5334–5338. [PMC free article] [PubMed]
  • Mills DR, Kramer FR, Dobkin C, Nishihara T, Cole PE. Modification of cytidines in a Q beta replicase template: analysis of conformation and localization of lethal nucleotide substitutions. Biochemistry. 1980 Jan 8;19(1):228–236. [PubMed]
  • Kramer FR, Mills DR, Cole PE, Nishihara T, Spiegelman S. Evolution in vitro: sequence and phenotype of a mutant RNA resistant to ethidium bromide. J Mol Biol. 1974 Nov 15;89(4):719–736. [PubMed]
  • Maniatis T, Jeffrey A, van deSande H. Chain length determination of small double- and single-stranded DNA molecules by polyacrylamide gel electrophoresis. Biochemistry. 1975 Aug 26;14(17):3787–3794. [PubMed]
  • Donis-Keller H, Maxam AM, Gilbert W. Mapping adenines, guanines, and pyrimidines in RNA. Nucleic Acids Res. 1977 Aug;4(8):2527–2538. [PMC free article] [PubMed]
  • Sanger F, Nicklen S, Coulson AR. DNA sequencing with chain-terminating inhibitors. Proc Natl Acad Sci U S A. 1977 Dec;74(12):5463–5467. [PMC free article] [PubMed]
  • Mills DR, Kramer FR. Structure-independent nucleotide sequence analysis. Proc Natl Acad Sci U S A. 1979 May;76(5):2232–2235. [PMC free article] [PubMed]
  • Khan MS, Maden BE. Conformation of mammalian 5.8 S ribosomal RNA: S1 nuclease as a probe. FEBS Lett. 1976 Dec 15;72(1):105–110. [PubMed]
  • Wurst RM, Vournakis JN, Maxam AM. Structure mapping of 5'-32P-labeled RNA with S1 nuclease. Biochemistry. 1978 Oct 17;17(21):4493–4499. [PubMed]

Articles from Nucleic Acids Research are provided here courtesy of Oxford University Press

Formats:

Related citations in PubMed

See reviews...See all...

Cited by other articles in PMC

See all...

Links

  • MedGen
    MedGen
    Related information in MedGen
  • PubMed
    PubMed
    PubMed citations for these articles
  • Substance
    Substance
    PubChem Substance links

Recent Activity

Your browsing activity is empty.

Activity recording is turned off.

Turn recording back on

See more...