Logo of narLink to Publisher's site
Nucleic Acids Res. 1982 Dec 20; 10(24): 7895–7903.
PMCID: PMC327057

Cloning of cDNA sequences of hormone-regulated genes from the MCF-7 human breast cancer cell line.


cDNA clones corresponding to a mRNA whose level is rapidly increased by addition of oestradiol to the culture medium have been isolated by differential screening of a cDNA library from the breast cancer cell line MCF-7, which contains oestrogen receptors. Such clones will be useful in studies of the DNA sequences required for hormonal induction and to determine whether expression of the corresponding gene is in any way related to the cancerous state. We have also obtained a cDNA clone for a messenger whose level is apparently decreased by steroid hormones.

Full text

Full text is available as a scanned copy of the original print version. Get a printable copy (PDF file) of the complete article (994K), or click on a page image below to browse page by page. Links to PubMed are also available for Selected References.

Images in this article

Click on the image to see a larger version.

Selected References

These references are in PubMed. This may not be the complete list of references from this article.
  • Brooks SC, Locke ER, Soule HD. Estrogen receptor in a human cell line (MCF-7) from breast carcinoma. J Biol Chem. 1973 Sep 10;248(17):6251–6253. [PubMed]
  • Horwitz KB, McGuire WL. Estrogen control of progesterone receptor in human breast cancer. Correlation with nuclear processing of estrogen receptor. J Biol Chem. 1978 Apr 10;253(7):2223–2228. [PubMed]
  • Westley B, Rochefort H. A secreted glycoprotein induced by estrogen in human breast cancer cell lines. Cell. 1980 Jun;20(2):353–362. [PubMed]
  • Edwards DP, Adams DJ, Savage N, McGuire WL. Estrogen induced synthesis of specific proteins in human breast cancer cells. Biochem Biophys Res Commun. 1980 Apr 14;93(3):804–812. [PubMed]
  • Westley B, Rochefort H. Estradiol induced proteins in the MCF7 human breast cancer cell line. Biochem Biophys Res Commun. 1979 Sep 27;90(2):410–416. [PubMed]
  • Adams DJ, Edwards DP, McGuire WL. Estrogen regulation of specific messinger RNA's in human breast cancer cells. Biochem Biophys Res Commun. 1980 Dec 31;97(4):1354–1361. [PubMed]
  • Mairesse N, Devleeschouwer N, Leclercq G, Galand P. Estrogen-induced protein in the human breast cancer cell line MCF7. Biochem Biophys Res Commun. 1980 Dec 31;97(4):1251–1257. [PubMed]
  • Auffray C, Rougeon F. Purification of mouse immunoglobulin heavy-chain messenger RNAs from total myeloma tumor RNA. Eur J Biochem. 1980 Jun;107(2):303–314. [PubMed]
  • Cochet M, Perrin F, Gannon F, Krust A, Chambon P, McKnight GS, Lee DC, Mayo KE, Palmiter R. Cloning of an almost full-length chicken conalbumin double-stranded cDNA. Nucleic Acids Res. 1979 Jun 11;6(7):2435–2452. [PMC free article] [PubMed]
  • Gergen JP, Stern RH, Wensink PC. Filter replicas and permanent collections of recombinant DNA plasmids. Nucleic Acids Res. 1979 Dec 20;7(8):2115–2136. [PMC free article] [PubMed]
  • Rave N, Crkvenjakov R, Boedtker H. Identification of procollagen mRNAs transferred to diazobenzyloxymethyl paper from formaldehyde agarose gels. Nucleic Acids Res. 1979 Aug 10;6(11):3559–3567. [PMC free article] [PubMed]
  • Alwine JC, Kemp DJ, Parker BA, Reiser J, Renart J, Stark GR, Wahl GM. Detection of specific RNAs or specific fragments of DNA by fractionation in gels and transfer to diazobenzyloxymethyl paper. Methods Enzymol. 1979;68:220–242. [PubMed]
  • Thomas PS. Hybridization of denatured RNA and small DNA fragments transferred to nitrocellulose. Proc Natl Acad Sci U S A. 1980 Sep;77(9):5201–5205. [PMC free article] [PubMed]
  • Breathnach R, Mandel JL, Chambon P. Ovalbumin gene is split in chicken DNA. Nature. 1977 Nov 24;270(5635):314–319. [PubMed]

Articles from Nucleic Acids Research are provided here courtesy of Oxford University Press


Save items

Related citations in PubMed

See reviews...See all...

Cited by other articles in PMC

See all...


  • Compound
    PubChem chemical compound records that cite the current articles. These references are taken from those provided on submitted PubChem chemical substance records. Multiple substance records may contribute to the PubChem compound record.
  • MedGen
    Related information in MedGen
  • PubMed
    PubMed citations for these articles
  • Substance
    PubChem chemical substance records that cite the current articles. These references are taken from those provided on submitted PubChem chemical substance records.

Recent Activity

Your browsing activity is empty.

Activity recording is turned off.

Turn recording back on

See more...