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Nucleic Acids Res. Oct 25, 1982; 10(20): 6441–6450.
PMCID: PMC326934

A tandem repeat gene in a picornavirus.


Three closely related genes for the small genome-linked protein (VPg) of picornaviruses have been identified by sequence analysis as a tandem repeat in the genome of Foot and Mouth Disease Virus (FMDV), strain O1K. This unusual structure was also found in the genome of strain C1O, belonging to a different FMDV serotype. Predicted biochemical properties of the three VPg gene products are in excellent agreement with the data from protein analysis of a heterogeneous VPg population from a third FMDV serotype, strain A10 (1). Taken together, these data indicate that the VPgs from all three genes function equally well in vivo. This is the first report of a tandem repeat gene in a viral genome.

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Selected References

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  • King AM, Sangar DV, Harris TJ, Brown F. Heterogeneity of the genome-linked protein of foot-and-mouth disease virus. J Virol. 1980 Jun;34(3):627–634. [PMC free article] [PubMed]
  • Wimmer E. Genome-linked proteins of viruses. Cell. 1982 Feb;28(2):199–201. [PubMed]
  • Rothberg PG, Harris TJ, Nomoto A, Wimmer E. O4-(5'-uridylyl)tyrosine is the bond between the genome-linked protein and the RNA of poliovirus. Proc Natl Acad Sci U S A. 1978 Oct;75(10):4868–4872. [PMC free article] [PubMed]
  • Ambros V, Baltimore D. Protein is linked to the 5' end of poliovirus RNA by a phosphodiester linkage to tyrosine. J Biol Chem. 1978 Aug 10;253(15):5263–5266. [PubMed]
  • Kitamura N, Adler CJ, Rothberg PG, Martinko J, Nathenson SG, Wimmer E. The genome-linked protein of picornaviruses. VII. Genetic mapping of poliovirus VPg by protein and RNA sequence studies. Cell. 1980 Aug;21(1):295–302. [PubMed]
  • Semler BL, Anderson CW, Kitamura N, Rothberg PG, Wishart WL, Wimmer E. Poliovirus replication proteins: RNA sequence encoding P3-1b and the sites of proteolytic processing. Proc Natl Acad Sci U S A. 1981 Jun;78(6):3464–3468. [PMC free article] [PubMed]
  • Kitamura N, Semler BL, Rothberg PG, Larsen GR, Adler CJ, Dorner AJ, Emini EA, Hanecak R, Lee JJ, van der Werf S, et al. Primary structure, gene organization and polypeptide expression of poliovirus RNA. Nature. 1981 Jun 18;291(5816):547–553. [PubMed]
  • Racaniello VR, Baltimore D. Molecular cloning of poliovirus cDNA and determination of the complete nucleotide sequence of the viral genome. Proc Natl Acad Sci U S A. 1981 Aug;78(8):4887–4891. [PMC free article] [PubMed]
  • Küpper H, Keller W, Kurz C, Forss S, Schaller H, Franze R, Strohmaier K, Marquardt O, Zaslavsky VG, Hofschneider PH. Cloning of cDNA of major antigen of foot and mouth disease virus and expression in E. coli. Nature. 1981 Feb 12;289(5798):555–559. [PubMed]
  • Roberts RJ, Breitmeyer JB, Tabachnik NF, Myers PA. A second specific endonuclease from Haemophilus aegyptius. J Mol Biol. 1975 Jan 5;91(1):121–123. [PubMed]
  • Grunstein M, Hogness DS. Colony hybridization: a method for the isolation of cloned DNAs that contain a specific gene. Proc Natl Acad Sci U S A. 1975 Oct;72(10):3961–3965. [PMC free article] [PubMed]
  • Clewell DB, Helinski DR. Supercoiled circular DNA-protein complex in Escherichia coli: purification and induced conversion to an opern circular DNA form. Proc Natl Acad Sci U S A. 1969 Apr;62(4):1159–1166. [PMC free article] [PubMed]
  • Maxam AM, Gilbert W. Sequencing end-labeled DNA with base-specific chemical cleavages. Methods Enzymol. 1980;65(1):499–560. [PubMed]
  • Sanger F, Coulson AR. The use of thin acrylamide gels for DNA sequencing. FEBS Lett. 1978 Mar 1;87(1):107–110. [PubMed]
  • Garoff H, Ansorge W. Improvements of DNA sequencing gels. Anal Biochem. 1981 Aug;115(2):450–457. [PubMed]
  • Osterburg G, Glatting KH, Sommer R. Computer programs for the analysis and the management of DNA sequences. Nucleic Acids Res. 1982 Jan 11;10(1):207–216. [PMC free article] [PubMed]
  • Kurz C, Forss S, Küpper H, Strohmaier K, Schaller H. Nucleotide sequence and corresponding amino acid sequence of the gene for the major antigen of foot and mouth disease virus. Nucleic Acids Res. 1981 Apr 24;9(8):1919–1931. [PMC free article] [PubMed]
  • Sangar DV. The replication of picornaviruses. J Gen Virol. 1979 Oct;45(1):1–13. [PubMed]
  • Sangar DV, Rowlands DJ, Harris TJ, Brown F. Protein covalently linked to foot-and-mouth disease virus RNA. Nature. 1977 Aug 18;268(5621):648–650. [PubMed]
  • Harris TJ, Brown F, Sangar DV. Differential precipitation of foot and mouth disease virus proteins made in vivo and in vitro by hyperimmune and virus particle guinea pig antisera. Virology. 1981 Jul 15;112(1):91–98. [PubMed]
  • Boothroyd JC, Harris TJ, Rowlands DJ, Lowe PA. The nucleotide sequence of cDNA coding for the structural proteins of foot-and-mouth disease virus. Gene. 1982 Feb;17(2):153–161. [PubMed]
  • King AM, McCahon D, Slade WR, Newman JW. Recombination in RNA. Cell. 1982 Jul;29(3):921–928. [PubMed]
  • Semler BL, Anderson CW, Hanecak R, Dorner LF, Wimmer E. A membrane-associated precursor to poliovirus VPg identified by immunoprecipitation with antibodies directed against a synthetic heptapeptide. Cell. 1982 Feb;28(2):405–412. [PubMed]

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