• We are sorry, but NCBI web applications do not support your browser and may not function properly. More information
Logo of narLink to Publisher's site
Nucleic Acids Res. Apr 25, 1983; 11(8): 2313–2323.
PMCID: PMC325886

Chromosomal mapping of Xenopus 5S genes: somatic-type versus oocyte-type.


Xenopus 5S RNA genes exhibit a pattern of differential expression during development in which some members (oocyte-type) are transcribed only in oocytes, while others (somatic-type) are expressed in both oocytes and somatic cells. Using cloned DNA probes specific for each gene type, we determined the positions of these genes on Xenopus metaphase chromosomes by in situ hybridization. Somatic-type 5S genes in both X. laevis and X. borealis are located at the distal end of the long arm of only one chromosome (number 9). The oocyte-type 5S RNA genes are found at the distal ends of the long arms of most Xenopus chromosomes, including chromosome 9. Thus, large scale differences in chromosomal location cannot explain the selective expression of these genes, as suggested previously.

Full text

Full text is available as a scanned copy of the original print version. Get a printable copy (PDF file) of the complete article (1.2M), or click on a page image below to browse page by page. Links to PubMed are also available for Selected References.

Images in this article

Selected References

These references are in PubMed. This may not be the complete list of references from this article.
  • Brown DD, Sugimoto K. The structure and evolution of ribosomal and 5S DNAs in Xenopus laevis and Xenopus mulleri. Cold Spring Harb Symp Quant Biol. 1974;38:501–505. [PubMed]
  • Ford PJ, Southern EM. Different sequences for 5S RNA in kidney cells and ovaries of Xenopus laevis. Nat New Biol. 1973 Jan 3;241(105):7–12. [PubMed]
  • Wegnez M, Monier R, Denis H. Sequence heterogeneity of 5 S RNA in Xenopus laevis. FEBS Lett. 1972 Sep 1;25(1):13–20. [PubMed]
  • Peterson RC, Doering JL, Brown DD. Characterization of two xenopus somatic 5S DNAs and one minor oocyte-specific 5S DNA. Cell. 1980 May;20(1):131–141. [PubMed]
  • Brown DD, Sugimoto K. 5 S DNAs of Xenopus laevis and Xenopus mulleri: evolution of a gene family. J Mol Biol. 1973 Aug 15;78(3):397–415. [PubMed]
  • Pardue ML, Brown DD, Birnstiel ML. Location of the genes for 5S ribosomal RNA in Xenopus laevis. Chromosoma. 1973;42(2):191–203. [PubMed]
  • Pardue ML. Localization of repeated DNA sequences in Xenopus chromosomes. Cold Spring Harb Symp Quant Biol. 1974;38:475–482. [PubMed]
  • Ford PJ, Brown RD. Sequences of 5S ribosomal RNA from Xenopus mulleri and the evolution of 5S gene-coding sequences. Cell. 1976 Aug;8(4):485–493. [PubMed]
  • Ford PJ, Mathieson T. Control of 5S RNA synthesis in Xenopus laevis. Nature. 1976 Jun 3;261(5559):433–435. [PubMed]
  • Southern EM. Detection of specific sequences among DNA fragments separated by gel electrophoresis. J Mol Biol. 1975 Nov 5;98(3):503–517. [PubMed]
  • Harper ME, Saunders GF. Localization of single copy DNA sequences of G-banded human chromosomes by in situ hybridization. Chromosoma. 1981;83(3):431–439. [PubMed]
  • Harper ME, Ullrich A, Saunders GF. Localization of the human insulin gene to the distal end of the short arm of chromosome 11. Proc Natl Acad Sci U S A. 1981 Jul;78(7):4458–4460. [PMC free article] [PubMed]
  • Hayashi S, Gillam IC, Delaney AD, Tener GM. Acetylation of chromosome squashes of Drosophila melanogaster decreases the background in autoradiographs from hybridization with [125I]-labeled RNA. J Histochem Cytochem. 1978 Aug;26(8):677–679. [PubMed]
  • Lai EC, Woo SL, Dugaiczyk A, O'Malley BW. The ovalbumin gene: alleles created by mutations in the intervening sequences of the natural gene. Cell. 1979 Jan;16(1):201–211. [PubMed]
  • Chandler ME, Yunis JJ. A high resolution in situ hybridization technique for the direct visualization of labeled G-banded early metaphase and prophase chromosomes. Cytogenet Cell Genet. 1978;22(1-6):352–356. [PubMed]
  • Fedoroff NV, Brown DD. The nucleotide sequence of oocyte 5S DNA in Xenopus laevis. I. The AT-rich spacer. Cell. 1978 Apr;13(4):701–716. [PubMed]
  • Korn LJ, Brown DD. Nucleotide sequence of Xenopus borealis oocyte 5S DNA: comparison of sequences that flank several related eucaryotic genes. Cell. 1978 Dec;15(4):1145–1156. [PubMed]
  • Tymowska J, Kobel HR. Karyotype analysis of Xenopus muelleri (Peters) and Xenopus laevis (Daudin), Pipidae. Cytogenetics. 1972;11(4):270–278. [PubMed]
  • Brown DD, Carrol D, Brown RD. The isolation and characterization of a second oocyte 5s DNA from Xenopus laevis. Cell. 1977 Dec;12(4):1045–1056. [PubMed]
  • Korn LJ. Transcription of Xenopus 5S ribosomal RNA genes. Nature. 1982 Jan 14;295(5845):101–105. [PubMed]
  • Korn LJ, Gurdon JB, Price J. Oocyte extracts reactivate developmentally inert Xenopus 5S genes in somatic nuclei. Nature. 1982 Nov 25;300(5890):354–355. [PubMed]
  • Engelke DR, Ng SY, Shastry BS, Roeder RG. Specific interaction of a purified transcription factor with an internal control region of 5S RNA genes. Cell. 1980 Mar;19(3):717–728. [PubMed]
  • Pelham HR, Brown DD. A specific transcription factor that can bind either the 5S RNA gene or 5S RNA. Proc Natl Acad Sci U S A. 1980 Jul;77(7):4170–4174. [PMC free article] [PubMed]
  • Sakonju S, Brown DD. Contact points between a positive transcription factor and the Xenopus 5S RNA gene. Cell. 1982 Dec;31(2 Pt 1):395–405. [PubMed]
  • Pelham HR, Wormington WM, Brown DD. Related 5S RNA transcription factors in Xenopus oocytes and somatic cells. Proc Natl Acad Sci U S A. 1981 Mar;78(3):1760–1764. [PMC free article] [PubMed]

Articles from Nucleic Acids Research are provided here courtesy of Oxford University Press


Related citations in PubMed

See reviews...See all...

Cited by other articles in PMC

See all...


Recent Activity

Your browsing activity is empty.

Activity recording is turned off.

Turn recording back on

See more...