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Fig. 3.

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Cell microscopy of mating between C. tropicalis opaque a and α cells. (A) Mating zygotes formed by fusion between opaque a and α cells. MTLa and MTLα cells were incubated with FITC-ConA or Rhodamine-ConA to label cell walls green or red, respectively. Labeled cells were coincubated on Spider medium for 8 h, and zygote cells were identified by microscopy. (B) Nuclear fusion during mating between opaque a and α cells. Nuclei in parental MTLa and MTLα cells were labeled by expression of a histone-mCherry or histone-GFP fusion protein, respectively. Nuclear-labeled strains were coincubated on Spider medium for 5–24 h, and zygotes were identified by microscopy. (C) Scanning electron micrographs of C. tropicalis zygotes. (Scale bars: A and B, 10 μm; C, 4 μm.)

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